Transfection was conducted using the Lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s instructions. The control vector, HK2 overexpression vector, control shRNA, or HK2 shRNA was obtained from OriGene and Santa Cruz Biotechnology, respectively. Control siRNA, HK2 siRNA, control mimic, miR-145 mimic, miR-148a mimic, miR-497 mimic, control inhibitor, miR-145 inhibitor, miR-148a inhibitor, or miR-497 inhibitor, was purchased from Invitrogen. When cells achieved 70% confluence, the above plasmid, shRNA, siRNA, or miRNA mimic (or inhibitor) was transfected into CC cells for 48 h. Finally, stable HK2-overexpressing or HK2 knockdown CC cell lines were established by selecting cells using G418 (Sigma-Aldrich) or Puromycin (Sigma-Aldrich) for 4 weeks.
Mir 148a inhibitor
Manufactured by Thermo Fisher Scientific
The MiR-148a inhibitor is a laboratory reagent designed to selectively inhibit the expression of the microRNA miR-148a. It is a synthetic oligonucleotide that can be used as a research tool to study the functional role of miR-148a in various biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Control mimic, by Thermo Fisher Scientific (1 mentions)
Control inhibitor, by Thermo Fisher Scientific (1 mentions)
Hk2 overexpression vector, by OriGene (1 mentions)
Control shrna, by OriGene (1 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Mir 145 inhibitor, by Thermo Fisher Scientific (1 mentions)
Mir 497 inhibitor, by Thermo Fisher Scientific (1 mentions)
Mir 148a mimic, by Thermo Fisher Scientific (1 mentions)
Mir 145 mimic, by Thermo Fisher Scientific (1 mentions)
Mirna mimic, by Merck Group (1 mentions)
Control sirna, by Thermo Fisher Scientific (1 mentions)
Puromycin, by Merck Group (1 mentions)
Control vector, by OriGene (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
2 protocols using mir 148a inhibitor
1
Establishing cell lines for HK2 overexpression and knockdown
2
Lipofectamine-mediated Transfection for miR-148a and S1PR1 Analysis
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Transfection was performed using Lipofectamine® 2000 (Invitrogen Life Technologies), in accordance with the manufacturer’s instructions. For miR-148a functional analysis, the HepG2 cells were transfected with the scrambled miRNA as a negative control, miR-148a mimics or miR-148a inhibitor (Invitrogen Life Technologies). For S1PR1 functional analysis, the HepG2 cells were transfected with S1PR1-specific small interfering (si)RNA or pcDNA3.1-S1PR1 plasmid (Nlunbio, Changsha, China).
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