PBMCs were obtained from patients and healthy donors by Ficoll density gradient separation. PBMCs were stored in liquid nitrogen until analysis. Thawed PBMCs were stained with the following fluorochrome-labeled antibodies against the indicated cell surface antigen: CD3 (clone UCHT1) and CD4 (13B8.2; Beckman-Coulter); CD8 (SK1), CD19 (SJ25C1), CD20 (L27), CD27 (L128), CD28 (L293), and IgM (G20-127; BD Biosciences); CCR7 (150503; R&D Systems); IgD (rabbit F(ab′)2; Dako); CD45RA (MEM-56; Invitrogen Life Technologies). DAPI was added to discriminate between live and dead cells. Samples were analyzed on a BD Biosciences LSR II flow cytometer with DIVA software.
Cd4 13b8.2
Manufactured by Beckman Coulter
Sourced in United States
The CD4 (13B8.2) is a laboratory equipment product manufactured by Beckman Coulter. It is used for the detection and quantification of CD4+ T cells in biological samples. The product's core function is to provide a reliable and accurate means of measuring CD4+ T cell levels, which is a crucial marker for monitoring the immune system status.
Automatically generated - may contain errors
Lab products found in correlation
Cd3 clone ucht1, by Beckman Coulter (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Cd45ra 2h4, by Beckman Coulter (1 mentions)
Cd3 ucht 1, by Beckman Coulter (1 mentions)
Cd45 clone j33, by Beckman Coulter (1 mentions)
Cd7 8h8.1, by Beckman Coulter (1 mentions)
Cd25 b1, by Beckman Coulter (1 mentions)
Kaluza analysis software, by Beckman Coulter (1 mentions)
Immu 357, by Beckman Coulter (1 mentions)
2 protocols using cd4 13b8.2
1
Comprehensive PBMC Immunophenotyping Protocol
2
Multiparametric Flow Cytometry of Mucosal Lymphocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PBMCs or lymphocytes isolated from the colon or rectal mucosa were utilized for flow cytometry. Monoclonal antibodies against CD45 (clone J33; Beckman Coulter, Pasadena, CA, USA), CD3 (UCHT1, Beckman Coulter), CD4 (13B8.2; Beckman Coulter), CD8 (B9.11; Beckman Coulter), CD7 (8H8.1; Beckman Coulter), CD25 (B1.49.9; Beckman Coulter), CD30 (HRS4; Beckman Coulter), CD45RA (2H4; Beckman Coulter), CD56 (N901; Beckman Coulter), CD62L (DREG56; Beckman Coulter), CD127 (R34.34; Beckman Coulter), CCR4 (i.e., CD194; L291H4; BioLegend), HLADR (Immu-357; Beckman Coulter), and PD1 (CD279; PD1.3; Beckman Coulter) were employed. The immunostained cells were analyzed using FACScan (Navios flow cytometer, Beckman Coulter) and Kaluza analysis software (version 1.3; Beckman Coulter). Lymphocytes were separated by flow cytometry based on high CD45 antigen expression and forward and side scatter properties. Subsequently, the flow cytometry data were analyzed according to the percentage of cell populations detected in each quadrant on two-dimensional scatterplots. We calculated the percentages of CD4+, CD8+, CD56+, CD7+, PD1+, CCR4+, CD30+, and HLADR+ cells among CD3+ cells. We also assessed the percentages of Treg, CD45RA+, and CD62L+ cells among CD3+CD4+ cells and percentages of CD45RA+ and CD62L+ cells among CD3+CD4− cells. In this study, we defined CD3+CD4+CD25+CD127low/- cells as Treg cells.
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