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2 protocols using anti 14 3 3θ antibody

1

Cell Culture Reagents and Antibodies

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DMEM and fetal bovine serum (FBS) used in cell culture were purchased from Invitrogen. 3,3diaminobenzidine tetrachloride (DAB), 1,4-dithiothreitol (DTT), Dimethyl Sulfoxide (DMSO), Triton X-100, Paraformaldehyde, Bovine Serum Albumin, Ethanol, and KCl hematoxylin protease inhibitor cocktail, Penicillin and streptomycin were from Sigma. Blue RangeTM Prestained protein Molecular Marker was from Pierce. BCA Protein Assay Kit was from Thermo Fisher Scienti c. Anti-AFF4 antibody (cat. no. sc-390310; 1:1000) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA), anti-14-3-3θ antibody (cat. no. ab14749; 1:500) and anti-β-catenin rabbit polyclonal antibody (cat. no. ab16051,1:1,000) was purchased from Abcam. HRP-conjugated A nipure Goat Anti-Mouse IgG(H+L) (cat.no.SA00001-1;1:3000), HRP-conjugated A nipure Goat Anti-Rabbit IgG(H+L) (cat.no.SA00001-2;1:3000) were purchased from Proteintech (Chicago, Ill., USA). PrimeSTAR DNA Polymerase, EasyTaq DNA Polymerase, SacI, XabI and T4 DNA ligase were purchased from Takara Bio, Inc. (Otsu, Japan).
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2

Cell Culture Reagents and Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
DMEM and fetal bovine serum (FBS) used in cell culture were purchased from Invitrogen. 3,3diaminobenzidine tetrachloride (DAB), 1,4-dithiothreitol (DTT), Dimethyl Sulfoxide (DMSO), Triton X-100, Paraformaldehyde, Bovine Serum Albumin, Ethanol, and KCl hematoxylin protease inhibitor cocktail, Penicillin and streptomycin were from Sigma. Blue RangeTM Prestained protein Molecular Marker was from Pierce. BCA Protein Assay Kit was from Thermo Fisher Scienti c. Anti-AFF4 antibody (cat. no. sc-390310; 1:1000) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA), anti-14-3-3θ antibody (cat. no. ab14749; 1:500) and anti-β-catenin rabbit polyclonal antibody (cat. no. ab16051,1:1,000) was purchased from Abcam. HRP-conjugated A nipure Goat Anti-Mouse IgG(H+L) (cat.no.SA00001-1;1:3000), HRP-conjugated A nipure Goat Anti-Rabbit IgG(H+L) (cat.no.SA00001-2;1:3000) were purchased from Proteintech (Chicago, Ill., USA). PrimeSTAR DNA Polymerase, EasyTaq DNA Polymerase, SacI, XabI and T4 DNA ligase were purchased from Takara Bio, Inc. (Otsu, Japan).
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