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Glucosyl β c12 ceramide

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Glucosyl (β) C12 ceramide is a synthetic lipid compound used in various laboratory applications. It is a glycosylated ceramide with a 12-carbon fatty acid chain. This product can be utilized as a research tool in the study of lipid biochemistry, cell biology, and membrane structure and function.

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Lab products found in correlation

C12 ceramide, by Avanti Polar Lipids (3 mentions) Phosphatidylserine, by Avanti Polar Lipids (2 mentions) Phosphatidylethanolamine, by Avanti Polar Lipids (2 mentions) Phosphatidylinositol, by Avanti Polar Lipids (2 mentions) Lysophosphatidylcholine, by Avanti Polar Lipids (2 mentions) Lactosyl β c12 ceramide, by Avanti Polar Lipids (2 mentions) Blasticidin s, by Thermo Fisher Scientific (1 mentions) Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions) Potassium hydroxide, by Merck Group (1 mentions) Lc ms grade water, by Merck Group (1 mentions) 3 5 di tert 4 butylhydroxytoluene, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Ammonium formate, by Merck Group (1 mentions) Sphingomyelin, by Avanti Polar Lipids (1 mentions) Multiquant software, by AB Sciex (1 mentions) Heneicosanoic acid, by Merck Group (1 mentions) Cholesterol 2 2 3 4 4 6 d6, by Merck Group (1 mentions) Phosphatidic acid, by Avanti Polar Lipids (1 mentions) Phosphatidylglycerol, by Avanti Polar Lipids (1 mentions) Phosphatidylcholine, by Avanti Polar Lipids (1 mentions)

Close spelling variants of this product

Ceramides (7 citations) C12 ceramide (6 citations) Glucosyl (β)ceramide (2 citations)

4 protocols using glucosyl β c12 ceramide

1

Profiling Lipid Standards for Mass Spectrometry

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Cell culture reagents, Lipofectamine LTX and blasticidin-S were from Life Technologies. All HPLC solvents were MS grade (Carlo Erba Reagents, Italia). Phospholipid standards: C13:0 lysophosphatidylcholines (lysoPC); C25:0 phosphatidylcholines (PC); C12:0 sphingomyelin (SM); 12:0–13:0 phosphatidylserine (PS); 12:0–13:0 phosphatidylinositol (PI); 12:0–13:0 phosphatidylglycerol (PG); 12:0–13:0 phosphatidic acid (PA); 12:0–13:0 phosphatidylethanolamine (PE); C12 ceramide (Cer); glucosyl (β) C12 ceramide (GCer); lactosyl (β) C12 ceramide (LacCer); C17 mono-sulfo galactosyl(β) ceramide (D18:1/17:0) were purchased from Avanti Polar Lipids. All other reagents were from Sigma-Aldrich.
Buratta S., Urbanelli L., Sagini K., Giovagnoli S., Caponi S., Fioretto D., Mitro N., Caruso D, & Emiliani C. (2017). Extracellular vesicles released by fibroblasts undergoing H-Ras induced senescence show changes in lipid profile. PLoS ONE, 12(11), e0188840.
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2

Lipidomic Analysis of Cellular Sphingolipids

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Methanol, LC-MS grade water, 3,5-Di-tert-4-butylhydroxytoluene (BHT), and ammonium formate were from Sigma-Aldrich (Saint Louis, MO, USA). Ethanol and HPLC analytical grade chloroform were, respectively, from J.T. Baker (Center Valley, PA, USA) and Carlo Erba (Cornaredo, MI, Italy). Potassium hydroxide was from Merk Millipore (Burlington, MA, USA). Acetic and formic acid were from Fluka-Analitical (Honeywell, Morris Plains, NJ, USA).
Sphinganine (d17:0), sphinganine-1-phosphate (d17:0), C12 Ceramide, Sphingomyelin (d18:1/12:0), and Glucosyl (β) C12 Ceramide were from were from Avanti Polar Lipids (Alabaster, AL, USA).
Barbacini P., Casas J., Torretta E., Capitanio D., Maccallini G., Hirschler V, & Gelfi C. (2019). Regulation of Serum Sphingolipids in Andean Children Born and Living at High Altitude (3775 m). International Journal of Molecular Sciences, 20(11), 2835.
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3

Quantitative Sphingolipid Analysis in LNCaP Cells

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LNCaP cells were grown in 60 mm plates and treated with Etomoxir or vehicle for 24 h. Incubation was stopped by adding 1 vol. of methanol. To an aliquot representing 25% of the total sample, a mixture of internal standards was added: 20 μL of a solution containing 0.5 nmol each of 12:0-ceramide, 12:0-sphingomyelin, glucosyl(β)-C12-ceramide and Lactosyl(β)-C12-ceramide (Cer/Sph Mixture I; Avanti Polar Lipids, Alabaster, AL). After extracting lipids using Bligh & Dyer method (22 (link)), sphingolipids were analyzed by liquid chromatography/tandem mass spectrometry essentially as described (23 (link)). Data were analyzed using MultiQuant software from AB Sciex (Framingham, MA), and are presented as the ratios between the integrated area of the intensity peak of each analyte and the intensity peak of the corresponding internal standard.
Schlaepfer I.R., Rider L., Rodrigues L.U., Gijón M.A., Pac C.T., Romero L., Cimic A., Sirintrapun S.J., Glodé L.M., Eckel R.H, & Cramer S.D. (2014). Lipid catabolism via CPT1 as a therapeutic target for prostate cancer. Molecular cancer therapeutics, 13(10), 2361-2371.
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4

Quantitative Lipid Profiling Protocol

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All HPLC solvents were mass spectrometry grade (Carlo Erba Reagents, Italia). The internal standard for cholesterol and oxysterols quantification was cholesterol-2,2,3,4,4,6-d6 (SIGMA-ALDRICH) for fatty acids internal standards were heneicosanoic acid (C21:0) and uniformly labeled 13C-linoleic acid (C18:2) (SIGMA-ALDRICH). Phospholipid standards: C13:0 lysophosphatidylcholines (LysoPC); C25:0 phosphatidylcholines (PC); C12:0 sphingomyelin (SM); 12:0–13:0 phosphatidylserine (PS); 12:0–13:0 phosphatidylinositol (PI); 12:0–13:0 phosphatidylglycerol (PG); 12:0–13:0 phosphatidic acid (PA); 12:0–13:0 phosphatidylethanolamine (PE); C12 ceramide (Cer); glucosyl (β) C12 ceramide (GC); lactosyl (β) C12 ceramide (LacCer); C17 mono-sulfo galactosyl(β) ceramide (D18:1/17:0; GalCer) were purchased from Avanti Polar Lipids.
Bonacina F., Coe D., Wang G., Longhi M.P., Baragetti A., Moregola A., Garlaschelli K., Uboldi P., Pellegatta F., Grigore L., Da Dalt L., Annoni A., Gregori S., Xiao Q., Caruso D., Mitro N., Catapano A.L., Marelli-Berg F.M, & Norata G.D. (2018). Myeloid apolipoprotein E controls dendritic cell antigen presentation and T cell activation. Nature Communications, 9, 3083.
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