Cs 5100 automated coagulation analyzer

Following overnight fasting, blood was drawn for the measurement of blood glucose, hematocrit (Ht), platelet, plasminogen activator inhibitor 1 (PAI-1), fibrinogen, factor VII (FVII), factor VIII (FVIII) and prothrombin fragment 1 + 2 (F1 + 2), and for assessment using T-TAS.
Glucose levels were measured using a GA echo buffer solution, Glucose BP standard solution and automated glucose analyzer GA08III (A&T corporation, Kanagawa, Japan). The LPIA·tPAI test with the automated immunoanalyzer STACIA (LSI Medience Corporation, Tokyo, Japan) for PAI-1, Thrombo check Fib (L) with CS-5100 Automated Coagulation Analyzer (Sysmex Corporation, Hyogo, Japan) for fibrinogen, HemosIL RecombiPlasTin or HemosIL Synth Asil with ACL TOP automated analyzer (Instrumentation Laboratory, Bedford, MA) for FVII or FVII and Enzygnost with Microplate Reader Emax (Molecular Devices Japan, Tokyo, Japan) for F1 + 2 were applied respectively.

Cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein AI (ApoAI), apolipoprotein B (ApoB), immunoglobulins (IgG, IgA, IgM), albumin (ALB), C-reactive protein (CRP), complement proteins C3 and C4, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels, lactate dehydrogenase (LDH) along with other biochemical indicators, were measured using a biochemical analyzer (Beckman AU5400, Germany). CRP, IgG, IgA, IgM, C3, C4 were detected using the immunoturbidimetric method. Prothrombin time (PT), activated partial thromboplastin time (APTT), Fibrinogen and D-dimer were measured using Sysmex CS-5100 automated coagulation analyzer. Blood cell counts, hemoglobin, and other hematological parameters were determined using an automated hematology analyzer (Sysmex Corporation, Japan). All data were extracted from the hospital’s laboratory information system and medical record system.