1 heptadecanoyl 2hydroxy sn glycero 3 phosphocholine

Manufactured by Avanti Polar Lipids

Sourced in United States

1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine is a synthetic phospholipid compound. It is a structural component of cell membranes.

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Lab products found in correlation

2 protocols using 1 heptadecanoyl 2hydroxy sn glycero 3 phosphocholine

Lipid Extraction from Exosomes

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Lipids the exosomes were extracted using the Bligh and Dyer technique. 27 The chloroform-methanolaqueous (1:2:0.8, v/v/v) mixture was stirred at 4 °C for 30 min. Then, 10 mmol/L of Tris-HCl (pH 7.4) and chloroform were added. The mixtures were centrifuged for 15 min at 1000 × g. For internal standards, 1,2dilauroyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids, Alabaster, AL, USA) and 1-heptadecanoyl-2hydroxy-sn-glycero-3-phosphocholine and were added. The upper and the lower phases were collected using chloroform and nitrogen gas stream respectively. Lipids obtained by the above process were dissolved with chloroform-methanol (2:1, v/v) and stored at -20 °C.

Senda A., Kojima M., Watanabe A., Kobayashi T., Nakatsutsumi K., Yagi M., Morishita K., Aiboshi J., & Otomo Y. (2021). Investigating Closed Room Conspirators of Posttraumatic Inflammatory Response: Multi- Omics Profiles of Exosomes Derived from Intestinal Epithelial Cells Under Ischemia Reperfusion Injury.

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Phospholipid Standards for Lipidomics

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HPLC grade solvents were obtained from VWR International (Mississauga, Ontario, Canada). The standard phospholipids; 1,2-dinonanoyl-sn-glycero-3-phosphocholine (09:0 PC), 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (17:0 LPC), 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC), 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (PAPC), 1-stearoyl-2-linoleoyl-sn-glycero-3-phosphocholine (SLPC), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (SAPC), 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (PDHPC), 1-palmitoyl-2-(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC), 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PAzPC) and 1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine (PONPC) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC), 1-palmitoyl-2-(5’-keto-6’-octenedioyl)-sn-glycero-3-phosphocholine (KOdiA-PC) and 1-palmitoyl-2(4’-keto-dodec-3’-ene-dioyl)-sn-glycero-3-phosphocholine (KDdiA-PC) were purchased from Cayman Chemicals (Ann Arbor, Michigan, USA). All other chemicals were purchased from Sigma Aldrich (St. Louis, Missouri, USA).

Solati Z., Edel A.L., Shang Y., O K, & Ravandi A. (2018). Oxidized phosphatidylcholines are produced in renal ischemia reperfusion injury. PLoS ONE, 13(4), e0195172.

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