Human glioblastoma U251 and U87MG cell lines were obtained from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in DMEM (SH30022.01; HyClone) with 10% FBS (Thermo Fisher Scientific) and maintained at 37°C in a 5% CO2 humidified atmosphere.
U87mg
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China, United States
The U87MG is a cell line derived from a human glioblastoma, a type of brain tumor. It is a widely used model in cancer research and drug discovery. The U87MG cell line exhibits characteristics typical of glioblastoma cells, such as rapid growth and high invasiveness. Researchers utilize this cell line to study various aspects of glioblastoma biology and to test potential therapeutic agents.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (24 mentions)
U251mg, by National Collection of Authenticated Cell Cultures (20 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (16 mentions)
Ln229, by National Collection of Authenticated Cell Cultures (13 mentions)
U118mg, by National Collection of Authenticated Cell Cultures (5 mentions)
Shg44, by National Collection of Authenticated Cell Cultures (4 mentions)
U87mg, by Thermo Fisher Scientific (3 mentions)
Hek293t, by National Collection of Authenticated Cell Cultures (3 mentions)
Ln229, by American Type Culture Collection (3 mentions)
Dmem medium, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (3 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Shg 44, by Thermo Fisher Scientific (3 mentions)
Opti mem, by Thermo Fisher Scientific (3 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Snb19, by National Collection of Authenticated Cell Cultures (2 mentions)
Gl261, by National Collection of Authenticated Cell Cultures (2 mentions)
62 protocols using u87mg
1
Culturing Human Glioblastoma Cell Lines
2
Cell Line Maintenance and Genetic Manipulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two human cell lines (U87MG, U251) were obtained from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and used within 6–8 months. All cells had tested for mycoplasma contamination and were cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) with 10% fetal bovine serum (FBS, Biological, Industries) and 1% penicillin-streptomycin (10378016, Invitrogen, USA) at 37 °C with 5% CO2. Plasmids and siRNA were transfected into cells with Lipofectamine 3000 (L3000-008, Invitrogen, USA) according to the manufacturer’s instructions. lentivirus particles were directly added into target cells with 4 μg/ml polybrene (GM040901, Genomeditech, Shanghai, China). After two rounds of infection, cells were selected for at least 7 days by adding 10 μg/ml blasticidin (B9300, Solarbio Life Sciences, Beijing, China) or 1 μg/ml puromycin (P8230, Solarbio Life Sciences, Beijing, China) into the growth medium.
3
Glioma Tissue Collection and Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At least 30 specimens, comprising 10 grade I–II and 10 grade III–IV glioma tissues, as well as 10 normal brain tissues, were collected from patients who underwent surgical operation before anti-tumor treatments at Jinling Hospital. Non-tumorous brain tissues were extracted from the tumor border or normal areas of brain tissues removed from glioma patients. Before inclusion in the study, a written informed consent had been required from all patients. The study strictly complied with the guidelines of the Declaration of Helsinki, while ethical approval was obtained from the Ethics Committee of Jinling Hospital.
Human GBM cell lines, namely U87MG, A172, and U251MG, were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China), U118MG was obtained from the American Type Culture Collection (ATCC), while the human astrocyte cell lines (HA) were provided by the Institute of Basic Medical Sciences (Beijing, China). Cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 1% penicillin/streptomycin (HyClone, GE Healthcare Life Sciences, Logan, UT, USA) and 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), and then maintained in a humidified incubator at 37°C with 5% CO2.
Human GBM cell lines, namely U87MG, A172, and U251MG, were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China), U118MG was obtained from the American Type Culture Collection (ATCC), while the human astrocyte cell lines (HA) were provided by the Institute of Basic Medical Sciences (Beijing, China). Cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 1% penicillin/streptomycin (HyClone, GE Healthcare Life Sciences, Logan, UT, USA) and 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), and then maintained in a humidified incubator at 37°C with 5% CO2.
4
Culturing Human Glioblastoma and Astroglia Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human glioblastoma cell lines (U87MG, A172, U251, LN229, T98G, and U373) and a normal human astroglia (NHA) cell line were obtained from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences and were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) containing fetal bovine serum (FBS) at a final concentration of 10% at 37 °C with 5% CO2 in humidified incubators. For in vitro hypoxia experiments, cells were cultured under consistent 1% O2 hypoxic conditions.
5
Culturing Glioma Cell Lines with ATO
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human U87MG, U251, LN229 and SNB19 glioma cell lines were purchased from the Chinese Academy of Sciences Cell Bank, Shanghai. The cells were grown in Dulbecco's modi ed Eagle's medium (DMEM, Gibco) supplemented with 10% fetal Bovine serum (Gibco), 100U/ml penicillin and 100µg/ml streptomycin. Glioma cell lines were cultured in the humidi ed incubator with 5% CO2 at 37°C. ATO powder (Sigma, St Louis, Mo-Aldrich) was dissolved in 1mM sodium hydroxide (NaOH) and diluted to different concentrations with phosphate-buffered saline (PBS).
6
Human Glioma Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human glioma cell lines LN229, U251, U87 MG were purchased from the Chinese Academy of Sciences Cell Bank (Shanghai, China) and National Infrastructure of Cell Line Resource, cultured as described previously.23 Cells were cultured in whole media containing DMEM supplemented with 10% fetal bovine serum (Hyclone) and 1% penicillin–streptomycin (10,000 U/mL, Invitrogen, USA) in 5% CO2 in a humidified incubator at 37°C. All cell lines were regularly checked for mycoplasma contamination.
7
GBM and Monocyte Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human GBM cell lines U87MG and LN229 and the mouse GBM cell line GL261 were purchased from the Chinese Academy of Sciences Cell Bank (Shanghai, China). The human monocyte leukemia cell line (THP-1) was a kind gift from Professor Yuan Guo, Department of General Medicine, Shandong University. U87MG, LN229, GL261, and THP1 were cultured in Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific; Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific). THP-1 cells were treated with 200 nM phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich; St. Louis, MO, USA) for 24 h to allow for differentiation into macrophages in six-well plates. All cells were maintained at 37°C in a cell incubator containing 5% CO2.
8
Human Glioma and Astrocyte Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human glioma cell lines, U251MG and U87MG, as well as the HCN-1a human neuronal cell line were purchased from the Chinese Academy of Sciences Cell Bank. Glioma cells and HCN-1a cells were cultured as described [4 (link),6 (link),7 (link)]. Human primary astrocyte cultures were purchased from the iBS Cell Bank of Fudan University (Shanghai, China) [22 (link)]. The astrocytes were derived from the cerebral cortices of a single trauma patient. All the astrocytes were positive of glial fibrillary acidic protein (GFAP). Primary human astrocytes were maintained in astrocyte media as described [22 (link)].
9
Culturing Normal Human Astrocytes and Glioblastoma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Normal human astrocytes (NHAs) were obtained from Lonza (Basel, Switzerland) and cultured in astrocyte growth media (Lonza) containing 3% fetal bovine serum (FBS), 4.5% glucose and reagents from BulletKits (Lonza) in a humidified incubator at 37°C and 5% CO2. Human GBM cell lines U87-MG, U251 and HEK-293T were purchased from Chinese Academy of Sciences Cell Bank (Shanghai, People’s Republic of China), and were cultured in DMEM (Hyclone, Logan, UT, USA) supplemented with 100 units of penicillin/mL, 100 ng of streptomycin/mL as well as 10% FBS. All the cells were maintained at 37°C in a humidified atmosphere with 5% CO2.
10
Glioma Cell Culture and Maintenance
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human glioma cell lines, U87MG, U251G, and T98G were purchased from the Chinese Academy of Sciences cell bank (Shanghai, China), U118MG were purchased from Obio Technology Co., LTD (Shanghai, China). Glioma cell lines were culture in Dulbecco’s Modified Eagle Medium (DMEM) (17619004, Corning, USA), containing 10% fetal bovine serum (10099141C, Gibco, USA), incubated at 37°C in a humidified atmosphere containing 5% CO2. Normal human astrocytes (HA) were purchased from Cell Science and cultured in astrocyte medium (#1801, ScienCell, USA) incubated at 37°C humidified atmosphere containing 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!