The human HCC cell lines (Huh7, HepG2, QGY-7703, Bel-7404, Hep3B, PLC/PRF/5) were obtained from ATCC (American Type Culture Collection) and Cell Resource Center of Chinese Academy of Science. All the cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere containing 5% CO2. Micheliolide (MCL, purity 98%, Chunqiu biology, China) was dissolved in dimethylsulfoxide (DMSO) at a stock concentration of 10 mM. N-acetyl-L-cysteine (NAC, Sigma) was prepared at 1M.
Bel 7404
Manufactured by American Type Culture Collection
Sourced in United States, China
The BEL-7404 is a compact and versatile laboratory incubator designed for cell culture applications. It features a temperature range of 5°C above ambient to 60°C, with a temperature uniformity of ±0.5°C. The incubator has a capacity of approximately 7.4 cubic feet and can accommodate a variety of cell culture vessels. The BEL-7404 is constructed with stainless steel interior and a tempered glass door for easy monitoring of samples.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by American Type Culture Collection (28 mentions)
Smmc 7721, by American Type Culture Collection (16 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (15 mentions)
Hep3b, by American Type Culture Collection (14 mentions)
Bel 7402, by American Type Culture Collection (11 mentions)
Sk hep1, by American Type Culture Collection (9 mentions)
Mhcc97h, by American Type Culture Collection (6 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (5 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (5 mentions)
Qgy 7703, by American Type Culture Collection (4 mentions)
Dmem medium, by Thermo Fisher Scientific (4 mentions)
Plc prf 5, by American Type Culture Collection (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Hcclm3, by American Type Culture Collection (3 mentions)
Snu 449, by American Type Culture Collection (3 mentions)
Thle 3, by American Type Culture Collection (3 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Hl 7702, by American Type Culture Collection (3 mentions)
Hepg2, by Thermo Fisher Scientific (3 mentions)
40 protocols using bel 7404
1
Culturing Human Liver Cancer Cell Lines
2
Synthesis and Characterization of Multifunctional Nanoparticles
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Er(NO3)3·5H2O (99.9%), Yb(NO3)3·5H2O (99.9%), Y(NO3)3·6H2O (99.9%), NaOH (analytical purity AR), absolute ethanol (AR), hydroxyethyl cellulose (AR, viscosity of 20,000 mPa s), polyethyleneimine (PEI, M.W. = 10000, 99%), NH4F (98%), concentrated hydrochloric acid (AR, 37%), dimethyl sulfoxide (DMSO, AR), 1-(3-dimethylamino propyl)-3-ethyl-carbodiimide hydrochloride (EDC, 98%), N-hydroxysuccinimide (NHS, 98%), phosphate-buffered saline (PBS, pH = 7.2–7.4), chlorin E6 (Ce6, 94%), and 1,3-diphenylisobenzofuran (DPBF, 99.9%) were procured through Sigma-Aldrich™ (St. Louis, USA). Anti-EpCAM monoclonal antibody was procured through eBioscience (San Diego, USA). A cellular proliferation kit (CCK-8) was procured through Abcam™ (Shanghai, China). 4′,6-Diamidino-2-phenylindole, dihydrochloride (DAPI) was procured through Sigma-Aldrich Chemicals™ (Sydney, Australia).
The human hepatocellular carcinoma cell line BEL-7404 was obtained from ATCC (Shanghai, China), and the LO2 cell line was obtained from the Affiliated Hospital of Qingdao University, China. BEL-7404 cultures were grown within RPMI-1640 medium, and LO2 cells were grown within DMEM augmented by 10% fetal bovine serum (FBS)/1% penicillin-streptomycin at 37°C within a humidified atmosphere carrying 5% CO2.
The human hepatocellular carcinoma cell line BEL-7404 was obtained from ATCC (Shanghai, China), and the LO2 cell line was obtained from the Affiliated Hospital of Qingdao University, China. BEL-7404 cultures were grown within RPMI-1640 medium, and LO2 cells were grown within DMEM augmented by 10% fetal bovine serum (FBS)/1% penicillin-streptomycin at 37°C within a humidified atmosphere carrying 5% CO2.
3
Characterization of Hepatocellular Carcinoma Cell Lines
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Normal human hepatic cell line LO2 and human hepatocellular carcinoma cell lines Bel‐7404, SNU‐368, HLE, HLF, and Hep3B were purchased from ATCC (Manassas, VA, USA). All these HCC cell lines were authenticated by short‐tandem repeat (STR) DNA testing by the Air Force Medical University Center for DNA Typing and tested for mycoplasma contamination. Bel‐7404 and SNU‐368 were cultured in RPMI 1640 (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS, Invitrogen). LO2, HLE, HLF, and Hep3B were cultured in Dulbecco's modified Eagle medium (DMEM, Invitrogen) containing 10% FBS. All cells were incubated in humidified atmosphere of 5% CO2 at 37°C. DMSO was purchased from Sigma‐Aldrich (St. Louis, MO). Sorafenib was purchased from Bayer (Leverkusen, Germany).
4
Transfection and Selection of Human Liver Cancer Cell Lines
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Human liver cancer cell lines, Bel7404, HCCLM3, MHCC97L, HepG2.2.215 and HepG2 were purchased from ATCC. Cells were cultured in RPMI 1640 or DMEM supplemented with 10% FBS, 1% streptomycin and amphotericin B. All cell lines were cultured in 5% CO2 incubator at 37 °C.
Cells were seeded into 6-wells plates one day before transfection. Endofectin™-plus (Genecopoeia, Rockville, USA) was used to transfect the cells when the confluence of cells reached 80–90% using the transfection methods provided by the reagent supplier. Cells were harvested and reseeded into 100 mm plates one day after transfection. Geneticin and hygromycin was used to select for the drug-resistant cells and western blot was performed to confirm the positive cell clone.
Cells were seeded into 6-wells plates one day before transfection. Endofectin™-plus (Genecopoeia, Rockville, USA) was used to transfect the cells when the confluence of cells reached 80–90% using the transfection methods provided by the reagent supplier. Cells were harvested and reseeded into 100 mm plates one day after transfection. Geneticin and hygromycin was used to select for the drug-resistant cells and western blot was performed to confirm the positive cell clone.
5
Human Hepatocellular Carcinoma Cell Lines Cultivation
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The human HCC cell lines THLE-3, HepG2, Hep3B and SUN475 were obtained from RIKEN BioResource Center (Tsukuba, Japan) and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10 % FBS, 2 mM L-glutamine and 100 U/ml of penicillin and streptomycin in a 6-cm dish. BEL-7405, BEL-7404 and BEL-7402 were obtained from the ATCC (Manassas, VA, USA) and maintained in Mc-Coy’s 5a Medium with 10 % FBS, 2 mM L-glutamine and 100 U/ml of penicillin and streptomycin and in Roswell Park Memorial Institute medium 1640 with 10 % FBS, 2 mM L-glutamine and 100 U/ml of penicillin and streptomycin.
6
Cultivation of Liver Cell Lines
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Immortalized normal liver epithelial cell, THLE3, was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). The HCC cell lines (Hep3B, HepG2, BEL-7402, BEL-7404, SNU-398, SNU-449, Huh7, and QGY-7703), were purchased from the ATCC, were maintained in Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Invitrogen), 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen), within a humidified atmosphere containing 5% CO2 at 37°C. Normal hepatocytes s established from fresh specimens of normal hepatic tissue, which had been histopathologically diagnosed and verified by experienced pathologists.
7
Characterization of HCC Cell Lines
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HCC cell Huh-7 and BEL-7404 were purchased from the ATCC. Hepatoblastoma cell Sk-hep-1 and endothelial-derived cell Sk-Hep1 were purchased from the ATCC. All the above cell lines of HCC were cultured in DMEM (Biological Industries) þ 10% FBS (Biological Industries) þ 1% penicillin/streptomycin at 37 C with 5% CO 2 and saturated humidity. All cell lines used were cultured within 35 generations and regularly tested for Mycoplasma contamination by the Plasmo Test kit (InvivoGen, rep-pt1). The short tandem repeat analysis method was used to verify the identity of cell lines twice a year at the core institution.
8
Culturing Human Liver Cancer Cell Lines
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The human liver cancer cell lines (BEL-7402, BEL-7404, SMMC-7721, MHCC-97H, MHCC-97 L, HepG2, HCCLM3, and Hep-3B) and normal liver cells (L02) were obtained from the American Type Culture Collection (ATCC, VA, USA) and were cultured in High glucose Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin solution at 5% CO2 and 37 °C.
9
Cell Culture and Transfection Protocol
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HL-02 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China), Bel-7404, SK-Hep1, Bel-7402, SMMC-7721, and HepG2 were obtained from American Type Culture Collection (ATCC, USA), which also used in our previous studies.15 (link),16 (link) Cells were cultured in DMEM (HyClone, USA) with 10% fetal bovine serum and 1% penicillin-streptomycin in a 37°C cell culture incubator with 5% CO2. Short interfering RNAs (siRNAs) were synthesized by GenePharma (Shanghai, China). Lipofectamine 2000 (Invitrogen, USA) was used to transfect the MCM and negative control siRNAs into the cells according to the manufacturer’s suggestions. The specific siRNA primers are listed in Table S1
10
YTHDF1 Knockdown in Cancer Cell Lines
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Hep3B, HepG2, MHCC97H, MHCC97L, and HCCLM3 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). SMMC7721 and BEL7404 were obtained from American Type Culture Collection (Rockville, MD, USA). Cells were maintained in Dulbecco′s modified Eagle′s medium (GIBCO, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO, USA) and 1% penicillin/streptomycin solution. Plasmids for YTHDF1 knockdown were constructed by Dharmacon (CA, USA). The transfection was performed by Lipo3000 according to the manufacturer′s instruction. The sequences of the short hairpin RNAs (shRNAs) were listed as follows: Kd-YTHDF1-1, GAACAUGCCAGUUUCAAAG; Kd-YTHDF1-2, GGACAGUCAAAUCAGAGUA; Kd-YTHDF1-3, CGACAUCCACCGCUCCAUU; Kd-YTHDF1-4, AAGGAACGGCAGAGUCGAA; NC, UAAGGCUAUGAAGAGAUAC.
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