All reagents and solvents were obtained from commercial suppliers and used without further purification. AMD3100 octahydrochloride (Plerixafor®) was prepared as previously described [13 (link), 14 (link)]. A stock solution of Plerixafor® was prepared in phosphate buffered saline (PBS) and neutralized with 1 M NaOH. N-[11C]Methyl-AMD3465 was prepared as previously described [12 (link)]. C6 rat glioma cells (ATCC, Manassas, VA) were cultured in monolayers in Dulbecco’s Modified Eagle Medium, supplemented with 10% fetal calf serum. Cells were maintained in a humidified atmosphere with 5% CO2 at 37 °C.
Rat c6 glioma cells
Manufactured by American Type Culture Collection
Sourced in United States
Rat C6 glioma cells are a cell line derived from a chemically-induced brain tumor in a Wistar rat. They are commonly used in research as a model for glioblastoma, a type of brain cancer.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
3 aminopropyltriethoxysilane aptes, by Merck Group (2 mentions)
Anhydrous ethanol, by Sinopharm (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions)
Trypsin, by Thermo Fisher Scientific (2 mentions)
Deionized water, by Merck Group (2 mentions)
Concentrated ammonia aqueous solution, by Sinopharm (2 mentions)
Human embryonic kidney 293t cells, by American Type Culture Collection (2 mentions)
Tetraethyl orthosilicate, by Sinopharm (2 mentions)
Doxorubicin hydrochloride dox, by Sangon (2 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Hbm basal medium, by Lonza (1 mentions)
Hepatocyte plating medium, by Lonza (1 mentions)
Bt474 cells, by American Type Culture Collection (1 mentions)
Mcf 7 cell, by American Type Culture Collection (1 mentions)
Hek293 cell, by American Type Culture Collection (1 mentions)
Mda mb 231 cell, by American Type Culture Collection (1 mentions)
Sh sy5y cell, by American Type Culture Collection (1 mentions)
12 protocols using rat c6 glioma cells
1
Radiolabeled AMD3465 Glioma Cell Study
2
Culturing Diverse Cell Lines for Research
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Human pancreatic islets were obtained from the Integrated Islet Distribution Program (IIDP) funded by the National Institute of Diabetes and Digestive and Kidney Disease (NIDDK). Islets were cultured in low-glucose-containing CMRL1066 medium with supplements as described in suspension culture dish.17 (link) HEK293 (ATCC), C6 rat glioma cells (ATCC), and PD220 primary human fibroblast cells (OHSU Fanconi Anemia Research Materials) were cultured in DMEM with high glucose supplemented with 10% fetal bovine serum (FBS). MIN6 mouse insulinoma (ATCC) cells were grown in DMEM with high glucose and 15% FBS and αTC1 mouse clone 6 alpha cells (ATCC) in DMEM with low glucose (1 g/L), 15% HEPES, and nonessential amino acid. Primary human hepatocytes were plated in hepatocyte plating medium (Lonza) at a density of 80,000 cells/cm2, and media were changed to culture media consisting of HBM basal medium and HCM supplement (Lonza) 18 h after the plating. SH-SY5Y human neuroblastoma (ATCC) cells were grown in culture media recommended by ATCC.
3
Cell Culture Conditions for Diverse Cell Lines
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C6 rat glioma cells (ATCC #CCL-107), HEK293 cells (ATCC #CRL-1573), GT1-7 cells [62 (link)], CLU188 cells [63 (link)], SH-SY5Y cells (ATCC #CRL-2266), SK-N-BE2 cells (ATCC #CRL-2271), iNHA cells [63 (link)], MDA-MB-231 cells (ATCC #HTB-26), MDA-MB-436 cells (ATCC #HTB-130), MDA-MB-453 cells (ATCC #HTB-131), MCF7 cells (ATCC #HTB-22), 4T1 cells (ATCC #CRL-2539), BT474 cells (ATCC #HTB-20), CHO cells (ATCC #CCL-61), OVK18 cells [64 (link)], DU145 cells (ATCC #HTB-81), and HCCLM3 cells [65 (link)] were maintained as a monolayer culture on tissue culture dishes at 37 °C, 5% CO2, 100% humidity in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics. SH-SY5Y cells were differentiated in the presence of 10 μM retinoic acid for 1 week [66 (link)]. OC-k3 cells [67 (link)] were maintained as a monolayer on tissue culture dishes at 33 °C, 10% CO2, 100% humidity in DMEM supplemented with 10% heat-inactivated fetal bovine serum, 50 U/mL of recombinant mouse interferon-γ, and antibiotics. S1P and CYM-5478 were solubilized with bovine serum albumin (0.1% final concentration) prior to treatment.
4
Culturing Rat Alveolar Macrophages and Glioma Cells
5
Culturing Cancer Cell Lines and CAFs
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SAS, a squamous cell carcinoma cell line derived from the oral cavity, B16 mouse melanoma cells and C6 rat glioma cells were obtained from the American Type Culture Collection. These cancer cells were cultured in DMEM containing 1.0 g/L glucose, 10% FBS and penicillin–streptomycin (Sigma, St. Louis, MO, USA). Axcel cells were established from metastatic lesions of melanoma patients4 and cultured in RPMI‐1640 (Sigma) containing 10% FBS. Cancer‐associated fibroblasts (CAF) were obtained from the tumoral gastric wall and cultured in DMEM containing 4.5 g/L glucose and 10% FBS.24
6
Culturing Glioma Cells and Human Glioma Progenitor Cells
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C6 rat glioma cells were obtained from the American Type Culture Collection (Manassas, VA) and grown in high-glucose DMEM supplemented with 10% heat-inactivated fetal bovine serum and glutamine (Invitrogen, Carlsbad, CA). Graded brain tumor specimens were obtained with informed consent as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. hGPCs were isolated and cultured as described previously (Chong et al., 2009 (link)) as tumor spheres in high-glucose DMEM/F-12 (3:1) supplemented with sodium pyruvate, nonessential amino acids, penicillin/streptomycin, B27 supplement (Invitrogen), basic fibroblast growth factor (bFGF; 20 ng/ml), epidermal growth factor (EGF; 20 ng/ml; Gene-Ethics (Asia), Singapore), and heparin (5 μg/ml; Sigma-Aldrich, St. Louis, MO). For transfection and migration assays, hGPCs were cultured as monolayers on laminin (10 μg/ml)–coated Petri dishes for several days before transfection. HGPCs and C6 transfections were performed with a Neon electroporator (Invitrogen) as per manufacturer's recommendations.
7
Synthesis and Characterization of Multifunctional Silica Nanoparticles
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Anhydrous ethanol, concentrated ammonia aqueous solution (NH3•H2O, 25 wt%), tetraethyl orthosilicate (TEOS), Bis[3-(triethoxysilyl) propyl] tetrasulfide (TETS), Dimethyl sulfoxide (DMSO), cetyltrimethylammonium bromide (CTAB), concentrated hydrochloric acid (HCl, 37%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). 3-aminopropyltriethoxysilane (APTES) was purchased from Sigma-Aldrich (USA). Cell culture medium (Dulbecco’s Modified Eagle’s Medium, DMEM), Dimethyl sulfoxide (DMSO), penicillin, fetal bovine serum (FBS) and Trypsin were provided by Gibco (USA). Bovine serum albumin (BSA) was provided by Promag (USA). 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) and lactoferrin (Lf) was purchased from Nanjing Kengen (China). Doxorubicin hydrochloride (Dox) purchased from Sangon (China). All water was deionized water (Millipore, USA) with a resistivity of 18.2 MΩ/cm. Human embryonic kidney cells 293T, rat glioma cells C6 were purchased from American Type Culture Collection (ATCC) Cell bank.
8
Synthesis and Characterization of Multifunctional Silica Nanoparticles
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Anhydrous ethanol, concentrated ammonia aqueous solution (NH3•H2O, 25 wt%), tetraethyl orthosilicate (TEOS), Bis[3-(triethoxysilyl) propyl] tetrasulfide (TETS), Dimethyl sulfoxide (DMSO), cetyltrimethylammonium bromide (CTAB), concentrated hydrochloric acid (HCl, 37%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). 3-aminopropyltriethoxysilane (APTES) was purchased from Sigma-Aldrich (USA). Cell culture medium (Dulbecco’s Modified Eagle’s Medium, DMEM), Dimethyl sulfoxide (DMSO), penicillin, fetal bovine serum (FBS) and Trypsin were provided by Gibco (USA). Bovine serum albumin (BSA) was provided by Promag (USA). 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) and lactoferrin (Lf) was purchased from Nanjing Kengen (China). Doxorubicin hydrochloride (Dox) purchased from Sangon (China). All water was deionized water (Millipore, USA) with a resistivity of 18.2 MΩ/cm. Human embryonic kidney cells 293T, rat glioma cells C6 were purchased from American Type Culture Collection (ATCC) Cell bank.
9
Rat C6 Glioma Cell Assay
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Rat C6 glioma cells were from the American Type Culture Collection. Dulbecco's modified eagle's high-glucose medium (DMEM), foetal bovine serum (FBS), penicillin/streptomycin, phosphate buffer solution (PBS), and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) were obtained from Gibco-Invitrogen Ltd. (Paisley, UK); [1-14C]acetate was obtained from GE Healthcare (Little Chalfont, UK); [1-14C]acetyl-CoA, [3-14C]3-hydroxy-3-methylglutaryl-CoA ([3-14C]HMG-CoA) were obtained from PerkinElmer (Boston, MA). Primary antibodies for acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and α-tubulin were obtained from Cell Signaling Technologies (Boston, MA). The antibody against 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) as well as horseradish peroxidase-conjugated IgGs was obtained from Santa Cruz Biotechnology (Dallas, TX). All other reagents, obtained from Sigma-Aldrich, were of analytical grade.
10
Cytotoxicity Evaluation of Fe3O4-Ce6 Nanoparticles
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The cell lines (brain endothelial bEnd.3 cells and rat C6 glioma cells) presents in this study were obtained from American Type Culture Collection (ATCC, United States). The bEnd.3 and C6 cancer cells were incubated with Fe3O4-Ce6 nanoparticles at different concentrations for 24 h, and then the cells were washed with PBS. The cells were then incubated in cell culture medium containing CCK-8 agent for 2 h. The supernatant of treated cells was collected to measure the absorbance at 450 nm using a Thermo Scientific Multiskan MK3 ELISA reader (Thermo scientific, United States), and then the cell viabilities were calculated.
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