Human pharyngeal squamous cell carcinoma cell line FaDu (RRID: CVCL_1218), AMC-HN-9 (RRID: CVCL_5967), TU212 (RRID: CVCL_4915), 5-8F (RRID: CVCL_C528) and NP69SV40T (RRID: CVCL_F755) was purchased from cell bank of Chinese Academy of Sciences (shanghai, China). All cells were grown under standard cell culture conditions of 37°C 5% CO 2 and 95% humidity. The FaDu and NP69SV40T cells were cultured in Dulbecco's modi ed Eagle medium (DMEM, Gibco) with 10% fetal bovine serum (FBS, Gibco, USA), 100 IU/ml penicillin and 100µg/ml streptomycin and maintained at low passage number. AMC-HN-9 and 5-8F cells were cultured in 1640 (Gibco, USA). TU212 was cultured in DMEM/F12 (Gibco, USA). All cells were tested for mycoplasma contamination before use. All cell lines were genotyped to establish identity within 6 months of experimentation. Rapamycin was purchased from (Absin, shanghai), A 20 mM solution was prepared in dimethyl sulfoxide and stored at -20℃ for in vitro experiments.
Rapamycin
Manufactured by Absin
Sourced in China
Rapamycin is a macrolide compound used as a laboratory reagent. It functions as an immunosuppressant by inhibiting the mammalian target of rapamycin (mTOR) signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Tu212, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
4 nitrophenyl phosphate, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Dmem high glucose medium, by Thermo Fisher Scientific (1 mentions)
β actin, by Boster Bio (1 mentions)
2 protocols using rapamycin
1
Characterization of Head and Neck Cancer Cell Lines
2
ER Stress Response Pathway Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DMEM high glucose medium, fetal bovine serum (FBS), and the penicillin–streptomycin solution were all obtained from Gibco (Life Technologies, Grand Island, NY, USA). 4-Nitrophenyl phosphate, disodium salt (PNPP), Dimethyl sulfoxide (DMSO), and other reagents were purchased from Sigma (St. Louis, MO, USA). Antibodies include the following: anti-EIF2α (S51), (D9G8), anti-Bip (C50B12), anti-IRE1 (14C10), anti-Spliced-XBP-1 (D2C1F), anti-ATF-4 (D4B8), anti-ATF-6 (AZ8V), anti-PERK (D11A8), were purchased from Cell Signaling Technology (Danvers, MA, USA). anti-p-IRE1 (ser724, Novus, Colorado, USA), and β-actin (#BM3873, BOSTER Biological Technology, Wuhan, China). Inhibitors include the following: salicylaldehyde (S5391-100, Solarbio, Beijing, China) and rapamycin (abs47000712, Absin Bioscience Inc. Shanghai, China).
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