Mouse n2a cells

Mouse N2A cells were purchased from American Type Culture Collection (ATCC). N2A cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM), low glucose, GlutaMAX^TM, pyruvate (21885025, Thermo Fisher) supplemented with 10 percent fetal bovine serum (FBS; P30-1985, Pan Biotech) and 100 U/ml penicillin-streptomycin (P0607300, PAN Biotech) at 37 °C in a humidified atmosphere of 95 percent air and 5 percent CO₂.

Male APP/PS1 mice were purchased from the Jackson Laboratory (Bar Harbor, ME, NO. 034832). Adult male C57BL/6 mice were purchased from the National Resource Center of Model Mice (Nanjing, China). The Tau-Knockout (Tau−/−) mouse was generated using the CRISPR–Cas9 system by specifically knocking out the sequence of exon 5 (Beijing Biocytogen Co., Ltd., Beijing, China). Genotyping for Tau−/− mice was performed by multiplex polymerase chain reaction using a pair of primers (Forward primer: 5′-GCTACAGTGTGAGTGAGGTTCTAGC-3′; Reverse primer: 5′-GAACCACAGCTGCTTAGGGAAAAC-3′). The genetic background of Tau−/− mice is C57BL/6. All these mice and their nontransgenic littermates were bred in the Experimental Animal Central of Tongji Medical College, Huazhong University of Science and Technology. All these mice were housed under a 12-h light/dark cycle in a temperature (22–24 °C) and humidity (40–60%) controlled room with enough food and water. This study was approved by the Institutional Animal Care and Use Committee of the Huazhong University of Science and Technology. Mouse N2a cells and Human 293T cells were bought from the American Type Culture Collection (ATCC) bank (Manassas, VA, USA).