Mouse monoclonal antibody against cox 4

Primary antibodies included a human anti-nuclear antibody derived from autoimmune human serum that was determined to stain the nuclear lamina used at a 1:100 dilution, a mouse monoclonal antibody against COX IV from Abcam (Eugene, OR) used at a 1:1000 dilution, a rabbit polyclonal antibody against AIF (Cell Signaling, Danvers, MA) used at a 1:250 dilution for immunofluorescent microscopy, a mouse monoclonal antibody from Santa Cruz Biotechnology against AIF (sc-13116, Santa Cruz, CA) used for western blotting, a mouse monoclonal antibody against β-Actin (A5316, Sigma/Aldrich, St. Louis, MO) used for western blotting, a polyclonal rabbit anti-PAR antibody (#551813) from BD Pharmingen (San Jose, CA) used at a 1:400 dilution for microscopy and 1:2000 dilution for western blotting, a polyclonal goat anti-PARP-1 (N-20, Santa Cruz, CA) used at a 1:1000 dilution. Secondary antibodies used for immunofluorescence microscopy were anti-mouse or anti-rabbit whole IgG conjugated to Alexa Fluor 488 (AF488) or Alexa Fluor 568 (AF568) from Invitrogen used at 2 μg per ml. Secondary antibodies used for western blot analysis were anti-rabbit or anti-goat conjugated to horseradish peroxidase from Jackson ImmunoResearch Laboratories (West Grove, PA) and used at a concentration of 0.1 μg per ml.