Dfc290 microscope

Manufactured by Leica

Sourced in Germany

The Leica DFC290 is a digital microscope camera that captures high-quality images for various applications. It features a 2.8-megapixel CMOS sensor and supports image resolutions up to 1920 x 1440 pixels. The camera is designed for ease of use and integration with Leica microscopes.

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Lab products found in correlation

Microplate reader, by Azure Biosystems (1 mentions) Cm1100 cryostat, by Leica (1 mentions) Ara c, by Merck Group (1 mentions)

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DFC290 (79 citations)

5 protocols using dfc290 microscope

Mapping Viral Expression in BNST

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Animals that survived the audiogenic-induced seizure were deeply anesthetized and transcardially perfused with ice-cold PBS followed by 4% PFA. For animals that did not survive the seizure, the brain was removed and fixed in 4% PFA. For animals used in electrophysiology, the brain was removed, and the brainstem was sliced for recording. The remainder of the brain was placed in 4% PFA overnight for fixation.
Brains were coronally sectioned (30 μm) using a vibratome (Leica Biosytems, Wetzlar, Germany) and slices containing the BNST were mounted on microscope slides. Slides were then visualized on a Leica DFC290 microscope (Leica Biosystems, Wetzlar, Germany) to verify the viral expression. SigmaPlot was used to generate a heat map showing viral expression of the AAV-TeNT virus in bilaterally injected mice that survived.

Xia M., Owen B., Chiang J., Levitt A., Preisinger K., Yan W.W., Huffman R, & Nobis W.P. (2022). Disruption of Synaptic Transmission in the Bed Nucleus of the Stria Terminalis Reduces Seizure-Induced Death in DBA/1 Mice and Alters Brainstem E/I Balance. ASN NEURO, 14, 17590914221103188.

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Mapping Viral Expression in BNST

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Animals that survived the audiogenic-induced seizure were deeply anesthetized and transcardially perfused with ice-cold PBS followed by 4% PFA. For animals that did not survive the seizure or were used for electrophysiology, the brain was removed and fixed in 4% PFA.
Brains were coronally sectioned (30 μm) using a vibratome (Leica Biosytems, Wetzlar, Germany) and slices containing the BNST were mounted on microscope slides. Slides were then visualized on a Leica DFC290 microscope (Leica Biosystems, Wetzlar, Germany) to verify the viral expression. SigmaPlot was used to generate a heat map showing viral expression of the AAV-TeNT virus in bilaterally injected mice that survived.

Xia M., Owen B., Chiang J.C.B., Levitt A., Yan W.W., & Nobis W.P. (2021). Disruption of synaptic transmission in the Bed Nucleus of the Stria Terminalis reduces seizure-induced death in DBA/1 mice and alters brainstem E/I balance.

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Osteosarcoma Cytotoxicity via Doxorubicin

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Osteosarcoma cells were seeded in triplicates at a density 0.1 × 10⁵ cells per well of a Costar 96 well ultra-low attachment surface plate in DMEM supplemented with 10% FBS or without FBS. Doxorubicin was added at a concentration of 1 μM. After 8 days of culture, the viable cells were detected using WST-1 assay according to the manufacturer’s instructions. Absorbance was measured using Azure Biosystems microplate reader. Photographs of the colonies were taken using a Leica DFC290 microscope (Leica Microsystems, Wetzlar, Germany) with 4× magnification. The surface area of photographed colonies was calculated with ImageJ.

Gerardo-Ramírez M., Keggenhoff F.L., Giam V., Becker D., Groth M., Hartmann N., Straub B.K., Morrison H., Galle P.R., Marquardt J.U., Herrlich P, & Hartmann M. (2022). CD44 Contributes to the Regulation of MDR1 Protein and Doxorubicin Chemoresistance in Osteosarcoma. International Journal of Molecular Sciences, 23(15), 8616.

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Quantifying Tumor Hypoxia via Pimonidazole

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Cryo-samples of the tumors were cut on a Leica CM1100 cryostat (Leica Microsystems, Wetzlar, Germany) into 7 µm thick slices. For each tumor, 3–4 slices were stained with hematoxylin and eosin according to the standard protocol and examined using a Leica DFC290 microscope (Leica Microsystems, Wetzlar, Germany).
The hypoxic fraction in the tumor tissue was analyzed using pimonidazole hydrochloride (Hypoxyprobe-1, Chemicon International, Temecula, CA, USA). Hypoxyprobe-1 was administered to mice intraperitoneally at a dose of 60 mg/kg 90 min prior to sacrification. Cryosections were stained with IgG1 mouse monoclonal antibodies conjugated with FITC at a dilution of 1:200 at 37 °C in a wet chamber for 3 h. The obtained tumor slices were examined on a Leica DMIL 31 LED fluorescence microscope (Leica Microsystems, Wetzlar, Germany), fluorescence was excited at 488 nm, and a signal was recorded in the range of 500–540 nm. The relative hypoxic fraction was calculated in the ImageJ software (NIH, Bethesda, MY, USA) as a percentage of the pimonidazole-positive area of the total tumor area.

Parshina Y.P., Komarova A.D., Bochkarev L.N., Kovylina T.A., Plekhanov A.A., Klapshina L.G., Konev A.N., Mozherov A.M., Shchechkin I.D., Sirotkina M.A., Shcheslavskiy V.I, & Shirmanova M.V. (2022). Simultaneous Probing of Metabolism and Oxygenation of Tumors In Vivo Using FLIM of NAD(P)H and PLIM of a New Polymeric Ir(III) Oxygen Sensor. International Journal of Molecular Sciences, 23(18), 10263.

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Wound Healing Assay for Liver Cells

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Liver cell lines were grown on 6-well plates in DMEM, supplemented with 10% FBS. Scratches were generated in confluent cell monolayers with a 200 µL pipette tip, and cell debris was removed by washing with PBS. Afterwards, the cells were allowed to migrate into the generated gap in serum-free medium for 72 h. To inhibit proliferation, cytosine b-D-arabinofuranoside (AraC, Sigma-Aldrich, Taufkirchen, Germany) was added at a concentration of 10 µM prior to migration assay. The cells were photographed using a Leica DFC290 microscope (Leica Microsystems CMS GmbH, Mannheim, Germany), and the cell-free gap was measured using ImageJ software.

Gerardo-Ramírez M., Giam V., Becker D., Groth M., Hartmann N., Morrison H., May-Simera H.L., Radsak M.P., Marquardt J.U., Galle P.R., Herrlich P., Straub B.K, & Hartmann M. (2023). Deletion of Cd44 Inhibits Metastasis Formation of Liver Cancer in Nf2-Mutant Mice. Cells, 12(9), 1257.

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