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Biotinylated ulex europaeus agglutinin 1

Manufactured by Vector Laboratories
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Sourced in Denmark

Biotinylated Ulex Europaeus Agglutinin I is a lectin derived from the seeds of the common gorse plant, Ulex europaeus. It has a high affinity for terminal alpha-L-fucose residues on glycoproteins and glycolipids.

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Lab products found in correlation

Lionheart fx, by Agilent Technologies (1 mentions) Dylight 594 streptavidin, by Vector Laboratories (1 mentions)

Spelling variants of this product

Ulex Europaeus Agglutinin I (20 citations) Ulex europaeus agglutinin (8 citations) Biotinylated ulex europaeus agglutinin-1 (UEA-1, (2 citations)

2 protocols using biotinylated ulex europaeus agglutinin 1

1

Vascular Network Formation by HREs and ASCs

Cited 1 time
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Human Retinal Endothelial Cells (HREs; ACBRI 181, Cell Systems Corporation, Kirkland, WA) were co-cultured with Cd140b+ or CD140b− ASCs as described previously (8 (link), 15 (link)). Briefly, a mixture of 5×103 HREs and 6×104 of ASC (per cm2) were re-suspended and cultured in EGM2-MV medium with 5% FBS for 6 days with media exchange every 3 days. Co-cultures were treated with PDGF-bb (50 ng/mL). At the end of the experiment, vascular networks were visualized by staining the cultures with biotinylated Ulex Europaeus Agglutinin I (Vector labs, Burlingame, CA) followed by Streptavidin DyLight® 594 (Vector labs, Burlingame, CA). 3×3 image montage of each well was captured by means of 4× objective using LIONHEARTFX automated live cell imager (BioTek Instruments, Inc.). Images were processed for total tube length by ImageJ (FIJI) software and results were expressed as tube length (pixels)/field.
Periasamy R., Elshaer S.L, & Gangaraju R. (2018). CD140b (PDGFRβ) signaling in adipose-derived stem cells mediates angiogenic behavior of retinal endothelial cells. Regenerative engineering and translational medicine, 5(1), 1-9.
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2

Capillary Analysis Using Fluorescence

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Capillarization was analyzed using fluorescence microscopy. Transverse sections of the muscle biopsies were cut at a thickness of 8 μm and placed onto glass slides and first treated with Biotinylated Ulex Europaeus Agglutinin I (Vector Laboratories, Burlingame, CA) and later with Streptavidin 1:200 (Dako, Glostrup, Denmark). Subsequently pictures were taken of the muscle samples and analyzed on a computer (ImageJ) for capillary to fiber ratio.
Christensen P.M., Gunnarsson T.P., Thomassen M., Wilkerson D.P., Nielsen J.J, & Bangsbo J. (2015). Unchanged content of oxidative enzymes in fast-twitch muscle fibers and kinetics after intensified training in trained cyclists. Physiological Reports, 3(7), e12428.
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