Rabbit monoclonal anti-PMCA1 antibody (ab190355) and the isotype control (ab172730) were purchased from Abcam (Cambridge, MA) and used for Western blots, and flow cytometric studies. Mouse monoclonal anti-HSC70 antibody (sc-7298), mouse monoclonal anti-panPMCA1/4 antibody (sc-20028), goat polyclonal pan-PMCA4 antibody (Y20, sc-22080), rat monoclonal anti-CD9 antibody (sc-18869), and anti-phosphotyrosine antibody (PY99) which is a mouse monoclonal (sc-7020) were purchased from Santa Cruz Biotechnology (Dallas, TX). Also mouse monoclonal anti-phosphotyrosine antibody (clone 4G10) was purchased from Millipore (Temecula, CA). A rabbit anti-mouse SPAM1 antiserum, a polyclonal antipeptide ([C]NEKGNASRRKESSD in the C-terminus [#381–395] custom-made by Zymed (South San Francisco, CA) and previously reported to be specific for SPAM1 via peptide inhibition (Deng et al., 2000 (link); Zhang and Martin-DeLeon, 2003 (link)) was used. Secondary antibodies were purchased from Santa Cruz Biotech, Inc., Life Technologies or Molecular Probes Inc. (Eugene, OR). Fluoro-Gel II with DAPI (17985–50), used for immunofluorescence, was obtained from Electron microscope sciences (Hatfield, PA).
Mouse monoclonal anti phosphotyrosine antibody clone 4g10
Manufactured by Merck Group
Sourced in Canada
The mouse monoclonal anti-phosphotyrosine antibody (clone 4G10) is a laboratory reagent used for the detection and analysis of phosphorylated tyrosine residues in proteins. This antibody specifically recognizes and binds to phosphorylated tyrosine moieties, allowing researchers to study signaling pathways and protein modifications in various biological systems.
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Lab products found in correlation
Ab190355, by Abcam (1 mentions)
Ab172730, by Abcam (1 mentions)
Anti phosphotyrosine antibody py 99, by Santa Cruz Biotechnology (1 mentions)
Lowry dc protein assay, by Bio-Rad (1 mentions)
Enhanced chemi luminescence (ecl), by Cytiva (1 mentions)
Protein g agarose, by Thermo Fisher Scientific (1 mentions)
Complete protease inhibitor cocktail, by Merck Group (1 mentions)
2 protocols using mouse monoclonal anti phosphotyrosine antibody clone 4g10
1
Antibody Sources and Validation for Cell Studies
2
Phospho-RIP2 Protein Immunoprecipitation
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Cells were lysed in RIPA buffer (50 mM Tris–HCl (pH7.5), 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1% NP-40 and 0.25% Na-deoxycholate) supplemented with complete protease inhibitor cocktail (Sigma), fresh 1 mM PMSF, 100 μM N-(methoxysuccinyl)-Ala-Ala-Pro-Val chloromethylketone) and 1 μM Na3VO4. Cell lysates were clarified by centrifugation at 16,000×g for 10 minute. Protein concentrations were determined using Bio-Rad Dc protein Lowry assay (Bio-Rad). For immunoprecipitation, mouse monoclonal anti-phosphotyrosine antibody; clone 4G10 from Millipore was used to pull-down total tyrosine phosphorylated protein in combination with protein G-agarose (Invitrogen). After SDS-PAGE gel separation, samples were transferred to a PVDF membrane, probed with the antibody of interest and developed by ECL (Amersham Biosciences). Rabbit polyclonal antibody against RIP2 was purchased from Cayman, and Phospho-RIP2 (Ser176) antibody from Cell Signaling.
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