Normal human mammary cells MCF10A and human breast cancer cell lines, MCF7, T47D and BT-474 cell lines were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). MCF10A were cultured in special medium (Procell, China). MCF7 and T47D cells were maintained in DMEM high glucose medium (Hyclone, USA) supplemented with 10% fetal bovine serum (Gibco, USA). BT-474 cell were maintained in RPMI-1640 (Gibco, USA) supplemented with 20% fetal bovine serum (Gibco, USA), 10 μg/mL Insulin (Beyotime, China) and 2mM L-glutamine (Procell, China). Cells were incubated at 37 °C in an atmosphere of 5% CO2.
Special medium
Manufactured by Procell
Sourced in China
Special medium is a laboratory reagent designed for the cultivation and growth of microbial cultures. It provides the necessary nutrients and growth factors to support the development of a wide range of microorganisms in a controlled laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 10a, by Procell (2 mentions)
Insulin, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Procell (1 mentions)
Bt474, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by National Collection of Authenticated Cell Cultures (1 mentions)
Bt 474, by National Collection of Authenticated Cell Cultures (1 mentions)
Mcf 10a, by National Collection of Authenticated Cell Cultures (1 mentions)
Mcf 7 cells, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
3 protocols using special medium
1
Cultivation of Human Breast Cell Lines
2
Culturing MCF-7 and MCF-10a Cells
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MCF-7 breast cancer cells and MCF-10a normal breast epithelial cells purchased from Procell Life Science & Technology CO., Ltd. (Wuhan, China). The MCF-7cells were cultured in DMEM (Gibco, USA) containing 10 % fetal bovine serum, and the MCF-10a cells were cultured in special medium(Procell, China). The cells were incubated at 37 °C in a 4 % CO2 incubator.
3
Microglia Activation by Amyloid-Beta
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Primary mouse brain microglia (Procell Life Science and Technology, Wuhan, China) were passaged in a trigas incubator using special medium (Procell Life Science and Technology, Wuhan, China). Logarithmic microglia were collected and divided into Control, Aβ1-42, GAS-L and GAS-H groups. The Aβ1-42 group was intervened with 25 μM recombinant amyloid 1-42, which was dissolved in PBS and then incubated at 37°C for overnight cross-linking reaction. The GAS groups were pretreated with 10 μM and 20 μM GAS for 6 h, and then treated with 25 μM Aβ1-42.
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