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Pro kinetics program

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The Pro-Kinetics program is a software application designed to analyze and interpret kinetic data obtained from spectroscopic experiments. It provides tools for data processing, model fitting, and visualization to support researchers in understanding the dynamic behavior of molecular systems.

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Lab products found in correlation

8453 diode array spectrophotometer, by Hewlett-Packard (2 mentions) Sx 18mv stopped flow instrument, by Applied Photophysics (2 mentions)

2 protocols using pro kinetics program

1

Anaerobic Titration and Stopped-Flow Kinetics of SCD1

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UV-Vis spectra were recorded using a Hewlett-Packard 8453 diode-array spectrophotometer (Palo Alto, CA). SCD1 solution in a tenometer was made anaerobic by 5 cycles of 30 s of vacuum followed by 4.5 min of saturating with argon. This anaerobic SCD1 solution was then titrated with anaerobic stock of dithionite in an air-tight syringe. The time courses of A340 in the reaction of H2O2 with SCD1 were recorded with an Applied Photophysics (Leatherhead, UK) model SX-18MV stopped-flow instrument. The observed rates, kobs, were obtained by fitting the time courses to biphasic exponential function. The fast spectral changes were monitored using the rapid-scan accessory with the stopped-flow machine and the optical species were resolved using the Pro-Kinetics program provided by Applied Photophysics.
Shen J., Wu G., Pierce B.S., Tsai A.L, & Zhou M. (2023). Free ferrous ions sustain activity of mammalian stearoyl-CoA desaturase-1. bioRxiv.
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2

Kinetic Analysis of H2O2 Reaction with SCD1

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UV-Vis spectra were recorded using a Hewlett-Packard 8453 diode-array spectrophotometer (Palo Alto). SCD1 solution in a tonometer was made anaerobic by 5 cycles of 30 s of vacuum followed by 4.5 min of saturating with argon. This anaerobic SCD1 solution was then titrated with anaerobic stock of dithionite in an air-tight syringe. The time courses of A340 in the reaction of H2O2 with SCD1 were recorded with an Applied Photophysics (Leatherhead) model SX-18MV stopped-flow instrument. The observed rates, kobs, were obtained by fitting the time courses to biphasic exponential function. The fast spectral changes were monitored using the rapid-scan accessory with the stopped-flow machine, and the optical species were resolved using the Pro-Kinetics program provided by Applied Photophysics.
Shen J., Wu G., Pierce B.S., Tsai A.L, & Zhou M. (2023). Free ferrous ions sustain activity of mammalian stearoyl-CoA desaturase-1. The Journal of Biological Chemistry, 299(7), 104897.
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