Extracellular recordings were performed with an MEA data-acquisition system (Multi Channel Systems, Reutlingen, Germany). The MEA plates contained a matrix of 60 titanium nitride electrodes (30 μm in diameter) with an inter-electrode distance of 200 μm. The signal recorded with one of the extracellular electrodes reflects local changes of the membrane potential. The MEA plates were sterilized and coated with polyornithine/laminin/fibronectin. Standard measurements were acquired at 5 kHz (at 37°C). Recordings were performed 48 hr after plating, for 100 s at baseline, and at 5 min after 100 nM isoprenaline. The parameters including filed potential duration (ms), peak amplitude (μV) inter-spike interval duration (ms), and beating rate (beats/min) were analyzed using AxoScope software (Molecular Devices) and CardioMDA.
Mea data acquisition system
Manufactured by MultiSciences Biotech
Sourced in Germany
The MEA (Micro-Electrode Array) data acquisition system is a specialized laboratory equipment used for monitoring and recording electrical signals from live cells, particularly neurons and cardiomyocytes. It provides a platform for researchers to study the electrophysiological properties and responses of these cells in a controlled in-vitro environment.
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Lab products found in correlation
3 protocols using mea data acquisition system
1
Extracellular Recordings of Cardiac Cells
2
Cardiac Electrophysiology Profiling of riPSC-CMs
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Extracellular field potentials of riPSC-derived CMs were analyzed using a microelectrode array (MEA) data acquisition system (Multi Channel Systems). EBs on day 16 of differentiation were plated onto fibrin-coated (25 μg/mL) planar-type MEAs of 64 electrodes (TiN, diameter: 30 μm, electrode spacing 200 μm, Multi Channel Systems) and were cultured for two additional days in differentiation medium. Measurements were conducted at baseline and in the presence of isoprenaline (1 μM), quinidine (30 μM) and lidocaine (1 mM). The McRack software (Multi Channel Systems) was used to record and analyze field potentials.
3
Evaluating hiPSC-CM Electrophysiology with MEA
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A multielectrode array (MEA) data acquisition system (Multi Channel Systems, Reutlingen,
Germany) was used to record the extracellular field potential (FP) of hiPSC-CM. The MEA
plate is composed of 60 titanium nitride electrodes with an inter-electrode space of 200
µm. Beating spheroids were plated on ECM Gel-coated MEA plates and allowed to attach for
48-72 hours before recording. Baseline FP recording and drug testing were performed 30 ± 5
days post-differentiation. On the day of the experiment, the MEA plates were connected to
a head stage amplifier. FP was acquired at 2 kHz, and all recordings were performed at
37˚C. Signals were recorded for 60 seconds at baseline and 5 minutes after drug
application. All drugs were purchased from Sigma-Aldrich, otherwise stated. Stock
solutions were prepared daily in appropriate solvent and used at desired concentrations
made in RPMI/B27 medium. Data were analyzed by Cardio2D software (version 2.2.2.0,
Multichannel system MCS GmbH). FP durations (FPD) was normalized to beating rate using the
Bazett correction formula (corrected FPD [cFPD]=FPD/3 √ (RR interval)). Drugs
used in pharmacological studies are listed inTable S4 (See Supplementary Online
Information atwww.celljournal.org ).
Germany) was used to record the extracellular field potential (FP) of hiPSC-CM. The MEA
plate is composed of 60 titanium nitride electrodes with an inter-electrode space of 200
µm. Beating spheroids were plated on ECM Gel-coated MEA plates and allowed to attach for
48-72 hours before recording. Baseline FP recording and drug testing were performed 30 ± 5
days post-differentiation. On the day of the experiment, the MEA plates were connected to
a head stage amplifier. FP was acquired at 2 kHz, and all recordings were performed at
37˚C. Signals were recorded for 60 seconds at baseline and 5 minutes after drug
application. All drugs were purchased from Sigma-Aldrich, otherwise stated. Stock
solutions were prepared daily in appropriate solvent and used at desired concentrations
made in RPMI/B27 medium. Data were analyzed by Cardio2D software (version 2.2.2.0,
Multichannel system MCS GmbH). FP durations (FPD) was normalized to beating rate using the
Bazett correction formula (corrected FPD [cFPD]=FPD/3 √ (RR interval)). Drugs
used in pharmacological studies are listed in
Information at
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