Agilent 8890 7079b gc ms

GC-MS analysis relied on an Agilent 8890/7079B GC-MS (Agilent, Santa Clara, CA, USA). The GC injector was equipped with an inert SPME liner (inner diameter 0.75 mm, Supelco, Bellefonte, ON, Canada) and operated in the splitless mode (0.75 min) followed by split mode with a ratio of 1:50. Extracted compounds were separated using a Rxi-624Sil MS column (30 m × 0.32 mm, layer thickness 1.8 μm, Restek, Bellefonte, PA, USA) operated in constant helium flow at 1.4 mL min-1. The column temperature program was as follows: 37 °C for 12 min, followed by 5 °C min-1 up to 150 °C, then 10 °C min⁻¹ up to 290 °C, and finally remaining at 290 °C for 8 min. The untargeted VOC analysis relied on the mass spectrometer working in a SCAN mode with the associated m/z ranging from 20 up to 250. The peak integration was based on extracted m/z ratio chromatograms, and such an approach allowed for a separation of the majority of peaks of interest from their neighbors. The quadrupole rods, ion source, and transfer line were kept at 150 °C, 230 °C, and 280 °C, respectively.
VOC identification was performed using a two-step process. First, the spectrum of a peak was checked against the NIST mass spectral library database. Next, the NIST identification was confirmed by comparing the retention times of peaks of interest with retention times obtained for reference standards prepared as outlined above.

GC-MS analysis relied on an Agilent 8890/7079B GC-MS (Agilent, Santa Clara, CA, USA). The GC injector was equipped with an inert SPME liner (inner diameter 0.75 mm, Supelco, Canada) and operated in the splitless mode (0.75 min), followed by split mode with a ratio 1:50. Extracted compounds were separated using an Rxi-624Sil MS column (30 m × 0.32 mm, layer thickness 1.8 μm, Restek, Bellefonte, PA, USA) operated in constant helium flow at 1.4 mL min⁻¹. The column temperature programme was as follows: 37 °C for 12 min, followed by 5 °C min⁻¹ up to 150 °C, then 10 °C min⁻¹ up to 290 °C, and finally remaining at 290 °C for 8 min. The untargeted VOC analysis relied on the mass spectrometer working in a SCAN mode with the associated m/z ranging from 20 up to 250. The peak integration was based on extracted m/z ratio chromatograms, and such an approach allowed for a separation of the majority of peaks of interest from their neighbours. The quadrupole, ion source, and transfer line were kept at 150 °C, 230 °C and 280 °C, respectively.
VOC identification was performed using a two-step process. First, the spectrum of a peak was checked against the NIST mass spectral library database. Next, the NIST identification was confirmed by comparing the retention times of peaks of interest with retention times obtained for reference standards prepared as outlined above.