Fitc conjugated anti α2 integrin

FACS analysis was performed as described previously [17 (link)]. We used the following fluorescein isothiocyanate (FITC)-conjugated antibodies and primary antibodies: FITC-conjugated isotype control (Becton Dickinson), FITC-conjugated anti-α2-integrin (BioLegend), and anti-β1-integrin (Abcam). Mean fluorescence intensity (MFI) was calculated by subtracting the intensities of the controls.

Cells were harvested after treatment with Accutase^® cell-detachment solution (Merck Millipore, Billerica, MA, USA), and the dissociated single cells were incubated with primary antibodies or fluorescein isothiocyanate (FITC)-conjugated antibodies diluted in FACS buffer (0.5% [w/v] bovine serum albumin [BSA] and 0.1% [w/v] sodium azide in PBS) for 30 min on ice. After washing, the cell suspension was incubated with Alexa Fluor^® 488-conjugated secondary antibodies (Molecular Probes, Eugene, OR, USA) diluted in FACS buffer for 30 min on ice. Cell sorting and analysis were performed using a FACSAria™ Cell Sorter (Becton Dickinson, Franklin Lakes, NJ, USA). We used the following FITC-conjugated antibodies and primary antibodies: FITC-conjugated isotype control (Becton Dickinson), FITC-conjugated anti-α1-integrin (BioLegend, San Diego, CA, USA), FITC-conjugated anti-α2-integrin (BioLegend), anti-β1-integrin (Abcam, Cambridge, UK), and anti-CD24 (Santa Cruz Biotechnology, Dallas, TX, USA). Mean fluorescence intensities (MFIs) were obtained after subtracting the intensities of the controls.