The following plasmids, antibodies and oligos were used: rabbit anti-HA (6908, Sigma-Aldrich, St. Louis, MO, USA), mouse anti-HA(12CA5) (sc-57592, Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-GFP (G1546, Sigma-Aldrich, St. Louis), mouse anti-GAPDH (G8140, US Biological, Salem, MA, USA), mouse anti-ORF59 (gift from Dr. Bala Chandran), rabbit anti-control IgG (sc-2027, Santa Cruz Biotechnology), mouse anti-control IgG (sc-2025, Santa Cruz Biotechnology), rabbit anti-JMJD3 (3457S, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-UTX (33510S, Cell Signaling Technology), control LacZ oligo (Protein and Nucleic Acid Facility, Stanford University), and PAN oligos (Protein and Nucleic Acid Facility, Stanford University). Plasmids pCS2-UTX-Flag and pCS2-JMJD3-Flag were purchased from Addgene.
Rabbit anti utx
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-UTX is a primary antibody that specifically binds to the UTX (ubiquitously transcribed tetratricopeptide repeat, X chromosome) protein. UTX is a histone demethylase involved in the regulation of gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti gfp, by Merck Group (1 mentions)
Rabbit anti ha, by Merck Group (1 mentions)
Mouse anti ha 12ca5, by Santa Cruz Biotechnology (1 mentions)
Mouse anti gapdh, by US Biological (1 mentions)
Mouse anti control igg, by Santa Cruz Biotechnology (1 mentions)
Pierce ripa buffer, by Thermo Fisher Scientific (1 mentions)
Rabbit anti β actin, by Cell Signaling Technology (1 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Halt protease inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
C280 imager, by Azure Biosystems (1 mentions)
Supersignal west pico plus ecl kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using rabbit anti utx
1
Plasmid and Antibody Protocol for Cell Signaling
2
Protein Extraction and Western Blot Analysis
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Protein was extracted from enriched primary splenic NK cells using Pierce RIPA buffer (Thermo Fisher) with Halt protease inhibitor cocktail (Thermo Fisher) and protein concentration was quantified using the Pierce BCA Protein Assay kit (Thermo Fisher). Samples were electrophoresed on NuPage Novex 4-12% Bis-Tris Protein Gels, transferred to PVDF membranes, and blocked overnight at 4 °C with 5% wt/vol nonfat milk in 1× TBS and 0.1% Tween-20. Immunoblots were performed using rabbit anti-UTX (1:1,000 dilution; Cell Signaling Rabbit monoclonal antibody, 33510), rabbit anti-β-actin (1:10,000 dilution; Cell Signaling, CST4970) and goat anti-rabbit horseradish peroxidase secondary antibody (1:20,000 dilution; Thermo Fisher, 31466). Proteins were detected using the SuperSignal West Pico PLUS ECL kit (Thermo Fisher) and visualized using the Azure Biosystems c280 imager.
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