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Precast sds polyacrylamide bistris gels

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Precast SDS polyacrylamide BisTris gels are laboratory equipment used for electrophoretic separation of proteins based on their molecular weight. These gels are pre-cast and pre-formulated for convenient and reproducible protein analysis.

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Lab products found in correlation

γ 32p atp, by PerkinElmer (2 mentions) Tween 20, by Thermo Fisher Scientific (1 mentions) Glutathione sepharose, by GE Healthcare (1 mentions) Triton x 100, by Merck Group (1 mentions) Ampicillin, by Merck Group (1 mentions) P81 phosphocellulose paper, by Cytiva (1 mentions)

3 protocols using precast sds polyacrylamide bistris gels

1

Protein Purification and Characterization

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Glutathione–Sepharose was from GE Healthcare. [γ32P]ATP was from PerkinElmer. Anti-FLAG M2–agarose, Tween 20, Colloidal Coomassie Blue staining kit and pre-cast SDS/polyacrylamide Bis-Tris gels were from Invitrogen. CHAPS was from Calbiochem. Triton X-100 was from Sigma. Ampicillin was from Merck. All peptides were synthesized by GL Biochem (Shanghai, China).
vanderWijst J., Blanchard M.G., Woodroof H.I., Macartney T.J., Gourlay R., Hoenderop J.G., Bindels R.J, & Alessi D.R. (2014). Kinase and channel activity of TRPM6 are co-ordinated by a dimerization motif and pocket interaction. Biochemical Journal, 460(Pt 2), 165-175.
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2

Peptide Synthesis and Staining Protocol

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Lumio Green, Colloidal Blue staining kit and precast SDS polyacrylamide BisTris gels were from Invitrogen. GL Biochem synthesized all peptides to a purity of >95%; peptide sequences were determined by mass spectrometry. Tissue-culture reagents were from Life Technologies. P81 phosphocellulose paper was from Whatman, and [γ-32P]-ATP was from PerkinElmer.
Zhang J., Siew K., Macartney T., O'Shaughnessy K.M, & Alessi D.R. (2015). Critical role of the SPAK protein kinase CCT domain in controlling blood pressure. Human Molecular Genetics, 24(16), 4545-4558.
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3

Optimized Protein Visualization via SDS-PAGE

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Lumio Green, Colloidal Blue staining kit and precast SDS polyacrylamide BisTris gels were from Invitrogen. All peptides were synthesized by GL Biochem to a purity of >95%; peptide sequences were determined by MS.
Schumacher F.R., Sorrell F.J., Alessi D.R., Bullock A.N, & Kurz T. (2014). Structural and biochemical characterization of the KLHL3–WNK kinase interaction important in blood pressure regulation. Biochemical Journal, 460(Pt 2), 237-246.
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