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Seahorse xf imaging and normalization system

Manufactured by Agilent Technologies
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The Seahorse XF Imaging and Normalization system is a lab equipment product by Agilent Technologies. It is designed to capture high-resolution images of cells and normalize data across samples.

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Lab products found in correlation

Seahorse xfe96 analyzer, by Agilent Technologies (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) D5030, by Merck Group (2 mentions)

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Seahorse XF system (2 citations)

2 protocols using seahorse xf imaging and normalization system

1

Measuring Mitochondrial Function in Brown Adipocytes

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Oxygen consumption rate (OCR) was measured using the Seahorse XFe96 Analyzer (Agilent) on day 8 of differentiation. One hour prior to respirometry, media was replaced with basal DMEM (Sigma Aldrich D5030) supplemented with 5 mM glucose, 2 mM GlutaMax TM (Fisher Scientific 13462629) and 2% fatty acid-free BSA (Sigma Aldrich A3803), and cells were incubated at 37°C in a non-CO2 incubator. NE (final concentration 2 μM) was injected to stimulate brown adipocytes. ATP-linked respiration and uncoupling were determined by injecting Oligomycin (final concentration 5 μM) and Antimycin A (final concentration 5 μM) respectively. Basal mitochondrial respiration in stimulated cells was determined by subtracting the non-mitochondrial respiration from NE-induced OCR. Raw data were normalized by in situ nuclear staining (Hoechst 33342, final concentration 10 μM) and in situ cell counting using Seahorse XF Imaging and Normalization system (Agilent). Normalized data were used for calculations.
Huang Z., Zhang Z., Moazzami Z., Heck R., Hu P., Nanda H., Ren K., Sun Z., Bartolomucci A., Gao Y., Chung D., Zhu W., Shen S, & Ruan H.B. (2022). Brown adipose tissue involution associated with progressive restriction in progenitor competence. Cell reports, 39(2), 110575.
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2

Measuring Mitochondrial Function in Brown Adipocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oxygen consumption rate (OCR) was measured using the Seahorse XFe96 Analyzer (Agilent) on day 8 of differentiation. One hour prior to respirometry, media was replaced with basal DMEM (Sigma Aldrich D5030) supplemented with 5 mM glucose, 2 mM GlutaMax TM (Fisher Scientific 13462629) and 2% fatty acid-free BSA (Sigma Aldrich A3803), and cells were incubated at 37°C in a non-CO2 incubator. NE (final concentration 2 μM) was injected to stimulate brown adipocytes. ATP-linked respiration and uncoupling were determined by injecting Oligomycin (final concentration 5 μM) and Antimycin A (final concentration 5 μM) respectively. Basal mitochondrial respiration in stimulated cells was determined by subtracting the non-mitochondrial respiration from NE-induced OCR. Raw data were normalized by in situ nuclear staining (Hoechst 33342, final concentration 10 μM) and in situ cell counting using Seahorse XF Imaging and Normalization system (Agilent). Normalized data were used for calculations.
Huang Z., Zhang Z., Moazzami Z., Heck R., Hu P., Nanda H., Ren K., Sun Z., Bartolomucci A., Gao Y., Chung D., Zhu W., Shen S, & Ruan H.B. (2022). Brown adipose tissue involution associated with progressive restriction in progenitor competence. Cell reports, 39(2), 110575.
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