3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt

Methanol, toluene, dichloromethane, ethyl acetate, tetrahydrofuran and petroleum ether were purchased from Concord (Tianjin, China). Methoxyl-poly(ethylene glycol) (mPEG₅₀₀₀) and hydrogen peroxide (H₂O₂) were purchased from Energy Chemical (Shanghai, China). 4,9-dibromo-6,7-bis(4-hexylphenyl)-[1,2,5]thiadiazolo[3,4-g]quinoxaline (DPTQ6-2Br) was purchased from Alfa (Zhengzhou, China). Cisplatin (purity 99%) was purchased from Shandong Boyuan Pharmaceutical Co., Ltd. (Shandong, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and sodium dodecyl sulfate (SDS) were purchased from Beyotime (Shanghai, China). Cell culture vessels were purchased from Corning (Corning, NY, USA). Roswell Park Memorial Institute -1640 (RPMI-1640) medium, fetal bovine serum (FBS), 0.25% trypsin-EDTA, and penicillin/streptomycin (P/S) were purchased from Gibco (Gran Island, NY, USA). 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine (DAPI) was purchased from Sigma-Aldrich (Shanghai, China). 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA), Annexin V-FITC/Propidium iodide (PI) apoptosis detection kit, Hoechst 33258 and Calcein-AM/PI double stain kit, ATP assay kit were purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Female Balb/c mice and female Balb/c nude mice (4–6 weeks) are used in the animal experiments.

Iron (II) chloride tetrahydrate (FeCl₂·4H₂O), iron (III) chloride hexahydrate (FeCl₃·6H₂O) and ammonia solution (NH₃·H₂O) were purchased from Shanghai Aladdin Regent Co., Ltd. (Shanghai, China). Zinc acetate dehydrate (Zn(OAc)₂·2H₂O), isopropanol, tetraethyl orthosilicate (TEOS) and methanol (CH₃OH), 3-aminopropyltriethoxysilane (APTES) (reagent grade, >98%) were purchased from Sigma-Aldrich. Cell Cycle and Apoptosis Analysis Kit, Prussian Blue-Neutral Red Staining Kit, and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Beyotime Biotech (Shanghai, China). Trypsin-EDTA solution and penicillin-streptomycin solution, fetal bovine serum (FBS) and DMEM medium were purchased from Gibco Life Technologies. All the other chemicals were of analytical grade, and Millipore water (18.2 MΩ) was used throughout the experiments.

Sodium selenite (Na₂SeO₃) and ascorbic acid (vitamin C) were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, PR China). Fetal bovine serum (FBS) and phosphate buffer saline (PBS) was purchased from Gibco (Life Technologies AG, Switzerland). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Invitrogen. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Beyotime. Annexin V-FITC Apoptosis Kit was purchased from Sinopharm (Shanghai, PR China). 4,6-Diamidino-2-phenylindole (DAPI) was purchased from Sigma (St. Louis, MO). The cyclic polypeptide Arg-GlyAsp-D-Phe-Cys (RGDfC) was purchased from China Polypeptides Co., Ltd. The siDCBLD2, siNC (Negative control, scramble sequence), FAM-labeled siDCBLD2, and cy5.5-labeled siDCBLD2 were obtained from RiboBio (Guangzhou, PR China), and the siDCBLD2 and siNC were as follows: 5′-GGCCAAAUCAGUGUUGUAAUU-3′ and 5′-UUCUCCGAACGUGUCACGUTT-3′. All the antibodies were purchased from CST (Massachusetts, US). All of the chemicals were analytical reagent grade and used according to previously published article [8 (link)].