Sprague dawley rats

Manufactured by Taconic Biosciences

Sourced in United States, Denmark

Sprague-Dawley rats are a widely used outbred albino rat strain. They are known for their docile temperament and robust growth characteristics, making them a popular choice for a variety of research applications.

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Lab products found in correlation

Rodent diet 5001, by LabDiet (3 mentions) C57bl 6 mice, by Taconic Biosciences (3 mentions) 2918 rodent chow, by Inotiv (3 mentions) C57bl 6 mice, by Jackson ImmunoResearch (2 mentions) Rat chow, by Inotiv (2 mentions) C3heb fej, by Jackson ImmunoResearch (2 mentions) C3h hej mice, by Jackson ImmunoResearch (2 mentions) Truetrack blood glucose monitoring system, by Nipro (2 mentions) Sprague dawley, by Taconic Biosciences (2 mentions) Ivc type 4, by Tecniplast (2 mentions) Sprague dawley rat, by Charles River Laboratories (2 mentions) Trypsin, by Thermo Fisher Scientific (2 mentions) Rodent chow 5053, by Purina (1 mentions) Penicillin streptomycin neomycin, by Thermo Fisher Scientific (1 mentions) Cb6f1 j mice, by Jackson ImmunoResearch (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Male nmri mice, by Taconic Biosciences (1 mentions) Isoflurane, by Baxter (1 mentions) Su5416, by Bio-Techne (1 mentions) C57b6 j, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Male Sprague-Dawley rats (80 citations) Female Sprague-Dawley rats (16 citations) Adult male Sprague-Dawley rats (12 citations) Sprague Dawley (7 citations) Sprague Dawley outbred rats (3 citations) Transgenic hTH-GFP Sprague Dawley rats (2 citations) Sprague Dawley neonatal rats (2 citations) Sprague Dawley rats (Rattus norvegicus; (2 citations)

190 protocols using sprague dawley rats

Amphetamine Pharmacokinetics in Rats

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Six male Sprague-Dawley rats (Taconic Farms, Hudson, NY) weighing 300–350 g served as subjects, and were housed individually in a temperature- and humidity-controlled environment under a 12-h light/dark cycle. All rats were fed approximately 10–15 g of standard rat chow 1h after each session in their home cages where water was continuously available. Thus, each subject was 22-hr food restricted prior to the start of each experimental session. Care of subjects was in accordance with the guidelines of the NIH and the NIDA IRP Animal Care and Use Program.
Five additional male Sprague-Dawley rats (Taconic Farms) weighing 300–350 g were used for the determination of plasma amphetamine concentrations. These animals were housed as described above with unrestricted access to water and food. Animal care and experimental manipulations were approved by the Institutional Animal Care and Use Committee at the Johns Hopkins University School of Medicine. Animal facilities at both Johns Hopkins and NIDA IRP are accredited by the AAALAC International.

Slezak J.M., Mueller M., Ricaurte G.A, & Katz J.L. (2018). Pharmacokinetic and pharmacodynamic analysis of d-amphetamine in an attention task in rodents. Behavioural pharmacology, 29(6), 551-556.

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Housing and Handling of C57BL/6 Mice and Sprague Dawley Rats

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Adult male C57BL/6 mice (Jackson Laboratory, Bar Harbor, ME) and adult male Sprague Dawley/SD rats (Taconic, Rensselaer, NY) were housed in a temperature- and humidity-controlled facility with a 12 hour light: 12-hour dark cycle. Animals had ad libitum access to food (LabDiet, Purina Rodent Chow 5053, St. Louis, MO) and water. All procedures utilizing experimental animals were conducted in accordance with the Guide for the Care and Use of Laboratory Animals, and experimental procedures were reviewed and approved by the Institutional Animal Care and Use Committee at Merck & Co., Inc., Kenilworth, NJ, USA.

Zhang J., Muise E.S., Han S., Kutchukian P.S., Costet P., Zhu Y., Kan Y., Zhou H., Shah V., Huang Y., Saigal A., Akiyama T.E., Shen X.L., Cai T.Q., Shah K., Carballo-Jane E., Zycband E., Yi L., Tian Y., Chen Y., Imbriglio J., Smith E., Devito K., Conway J., Ma L.J., Hoek M., Sebhat I.K., Peier A.M., Talukdar S., McLaren D.G., Previs S.F., Jensen K.K, & Pinto S. (2020). Molecular Profiling Reveals a Common Metabolic Signature of Tissue Fibrosis. Cell Reports Medicine, 1(4), 100056.

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Molecular and Pharmacological Studies in Sprague-Dawley Rats

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We used seventy-eight male Sprague-Dawley (SD) rats (250–300 g; Taconic, Germantown, NY, USA) for the experiments. These animals were housed in standard polycarbonate cages with chow and water ad libitum and maintained in a temperature- and humidity-controlled room on a 12/12-hour light/dark cycle. Efforts were made to minimize the number of animals used in these experiments and their discomfort. All experimental procedures were approved by the Institution Animal Care and Use Committee (Protocol MED-20-03) and performed following the National Institutes of Health Guide for the Care and Use of Laboratory Animals [39 ]. Figure 1 shows experimental designs and animal groups for molecular and pharmacological studies.

Akinfiresoye L.R., Newton J., Suman S., Datta K, & N’Gouemo P. (2022). Targeted inhibition of upregulated sodium-calcium exchanger in rat inferior colliculus suppresses alcohol withdrawal seizures. Molecular neurobiology, 60(1), 292-302.

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Neuronal Cell Culture Protocols from Rodent Tissues

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Postnatal day 5-7 (P5-P7) old Sprague-Dawley (SD) rats (RRID:RGD_7246927) were ordered from Taconic Farms Inc. (Germantown, NY). Six-week-old CB6F1/J mice (RRID:IMSR_JAX:100007) were ordered from Jackson Laboratory (Bar Harbor, ME). C57BL/6J mice (RRID:IMSR_JAX:000664) were obtained from the central breeding facility of the University Medical Center Hamburg-Eppendorf. Mice were maintained for breeding P6-P7 old offspring with ad libitum access to food and water and a 12 hour light and 12 hour dark cycle in the animal facility of the Division of Life Sciences at the Nelson Biology Laboratories of Rutgers University or at the University Medical Center Hamburg-Eppendorf. Rats and mice of either sex were used for primary cerebellar granule cell culture. All animal experiments were approved by the Institutional Animal Care and Use Committee of Rutgers University (protocol # 09-051) or by the responsible committee of the State of Hamburg (permission number ORG 679), and all experiments were conducted in compliance with the ARRIVE guidelines for reports on animal research.
Human IMR-32 neuroblastoma cells (cat# 300148/p666_IMR-32, RRID:CVCL_0346) were obtained from the National Center for Cell Science (Pune, India) and maintained in DMEM (Sigma-Aldrich) supplemented with 1× penicillin/streptomycin/neomycin (GIBCO) and 10% fetal bovine serum at 37°C and 5% CO₂.

Loers G., Astafiev S., Hapiak Y., Saini V., Mishra B., Gul S., Kaur G., Schachner M, & Theis T. (2017). The polysialic acid mimetics idarubicin and irinotecan stimulate neuronal survival and neurite outgrowth and signal via protein kinase C. Journal of neurochemistry, 142(3), 392-406.

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Rodent Models for Neuroscience Research

Cited 1 time

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Male NMRI mice (Taconic, Lille Skensved, Denmark) were used for microdialysis and c-fos experiments.^{4 (link)} Sprague Dawley rats, weighing 320–345 g, were also used for the study (Taconic). All other in vivo experiments used adult male C57BL/6 mice from Jackson Laboratories or bred internally at the Vanderbilt University. All the animals were kept at room temperature in a 12 h light/dark cycle with free access to food and water. All the experiments were performed during the light cycle. For immunohistochemical characterization of GLP-1R localization, bacterial artificial chromosome transgenic mice containing a fluorescent reporter expressed in GLP-1R-expressing cells were generated as described in Supplementary Information. All the experiments were in accordance with directives of ‘Principles of Laboratory Animal Care' (NIH publication No. 85–23) and approved by either the Danish Experimental Animal Inspectorate and the council of the European Communities or the Vanderbilt Institutional Animal Care and Use Committee.

Reddy I.A., Pino J.A., Weikop P., Osses N., Sørensen G., Bering T., Valle C., Bluett R.J., Erreger K., Wortwein G., Reyes J.G., Graham D., Stanwood G.D., Hackett T.A., Patel S., Fink-Jensen A., Torres G.E, & Galli A. (2016). Glucagon-like peptide 1 receptor activation regulates cocaine actions and dopamine homeostasis in the lateral septum by decreasing arachidonic acid levels. Translational Psychiatry, 6(5), e809-.

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Behavioral Regulation in Sprague-Dawley Rats

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Naive, male Sprague-Dawley rats (Taconic Biosciences, Inc., Cranbury, NJ) weighing 200–250g, at the start of the experiment, were initially group-housed under a reversed 12-h light/dark cycle with water available ad libitum (N=35) for a two week acclimation period. During the experiments, rats were individually housed, placed on a restricted food diet, and maintained at approximately 85% of their free feeding body weights by earning pellets in the experimental chambers and receiving approximately 15 g of Purina Rodent Chow each day after the session. All rats were maintained in accordance with the guidelines of the Institutional Animal Care and Use Committee of Temple University (Institution of Laboratory Animal Research, National Academy Press; Eighth edition, revised 2011).

Saber I., Milewski A., Reitz A.B., Rawls S.M, & Walker E.A. (2019). Effects of dopaminergic and serotonergic compounds in rats trained to discriminate a high and a low training dose of the synthetic cathinone mephedrone. Psychopharmacology, 236(3), 1015-1029.

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Sprague-Dawley Rat Behavioral Protocols

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All methods followed the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines. The experiments were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Texas at Dallas (protocol number 15-13) and by the Animal Care and the Use Review Office (ACURO) of the United States Army Medical Research and Materiel Command Office of Research Protections, and conducted according to the protocols and guidelines. Eighty adult male Sprague–Dawley rats (Taconic Biosciences, USA), approximately 3 months old and weighing 250–280 g at the beginning of testing, were used in these studies. After arrival, rats were acclimated to the housing room for a week, and then were individually housed in plastic home cages. Three days before the beginning of the experiments, each rat was handled for 5 min a day in order to acclimate the animals to the experimenters. The animals were always kept under standard controlled room temperature, humidity, and light cycle (12:12 h, lights on at 6 am) and had free access to food and water. All procedures were conducted during the light cycle. Before the beginning of every procedure, a 20 min acclimation to the respective experimental room was used to reduce arousal.

Souza R.R., Powers M.B., Rennaker R.L., McIntyre C.K., Hays S.A, & Kilgard M.P. (2022). Timing of vagus nerve stimulation during fear extinction determines efficacy in a rat model of PTSD. Scientific Reports, 12, 16526.

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Sprague-Dawley Rat Brain Tissue Sampling

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Brain tissue was harvested from 25 male Sprague-Dawley rats, 280–320 g (Taconic, Lille Skensved, Denmark), for inclusion in the BTB measurements. The animals were group housed at 20–22 °C under a 12 h light/dark cycle, with ad libitum access to food and water. Under deep anesthesia using isoflurane (Baxter Medical AB, Kista, Sweden), the rats were decapitated and brains were collected and immediately placed on ice. The brains were dissected in a sagittal plane by the longitudinal fissure. ROIs such as FrCx, PrCx, CRB, BG, and hippocampus (HIP) were sampled according to Glowinski and Iversen^{57 (link)}. The tissue was weighed and samples were stored at −20 °C. All animal procedures were performed in accordance with the guidelines of the Swedish National Board for Laboratory Animals and were approved by the local Animal Ethics Committee in Uppsala, Sweden (ethical approval C189/14).

Gustafsson S., Sehlin D., Lampa E., Hammarlund-Udenaes M, & Loryan I. (2019). Heterogeneous drug tissue binding in brain regions of rats, Alzheimer’s patients and controls: impact on translational drug development. Scientific Reports, 9, 5308.

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Neuroendocrine effects of exposure in rats

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Sprague–Dawley rats, 10 males and 23 females (Taconic, Ry, Denmark), 10–12 weeks old, were used. All experiments were performed in accordance with the Swedish National Guidelines for Animal Experiments, and approved by the Stockholm Animal Care and Use Ethics Committee (Stockholm Norra Djurförsöksetiska Nämnd). Animals were housed in groups of three or four in Type IV MakrolonR plastic cages under standardised conditions (12 h light/dark cycle, lights on at 07:00; temperature of 22 ± 0.5°C; and 40–50% relative humidity). Food and water were provided ad libitum to the animals.
Two separate experiments made up this study: Experiment #1 consisted of ISH and IHC analysis. Here, the female rats were sacrificed at two post-exposure time points: 1 day = 6+5, and 7 days = 6+6 exposed and sham, respectively. Additionally, ISH findings from a previously published studies of male rodents were used for comparison (47 (link), 48 (link)). Experiment #2 included several ELISAs with serum from female rats sacrificed at 1 day and 7 days post-exposure from Experiment #1, and serum from exposed and sham males sacrificed at 1 day post-exposure (n = 5, 5 exposed and sham).

Kawa L., Arborelius U.P., Hökfelt T, & Risling M. (2020). Sex-Specific Differences in Rodents Following a Single Primary Blast Exposure: Focus on the Monoamine and Galanin Systems. Frontiers in Neurology, 11, 540144.

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Sexually Dimorphic Effects of 6-OHDA Lesions

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A total of 22 female and 55 male Sprague-Dawley rats were used (Taconic Farms, Germantown, New York, USA). All subjects were between 2 and 2.5 months of age at the beginning of the experiment, and were 3–3.5 months old at the time of behavioral testing. All rats received bilateral neostriatal injections of either 6-hydroxydopamine (6-OHDA) or vehicle (below). The females were gonadally intact. Among the males, 22 were gonadally intact, 11 were gonadectomized (GDX), 11 were GDX and supplemented with testosterone propionate (GDX+TP), and 11 were GDX and supplemented with 17β-estradiol (GDX+E). Rats were pair-housed by sex in standard-sized tub cages (Lab Products, Inc., Seaford, DE, USA) under a 12-h non-reversed light-dark cycle with food (Purina PMI Lab Diet: ProLab RMH 3000) and water available ad libitum. The cages and water bottles were made from bisphenol–free plastic (Zyfone) and ground corncob bedding (Bed O' Cobs, The Anderson Inc., Maumee, Ohio, USA) was used. All procedures involving rats were approved by the Institutional Animal Care and Use Committee at Stony Brook University and were performed in accordance with the U.S. Public Health Service Guide for Care and Use of Laboratory Animals to minimize their discomfort.

Conner M.R., Jang D., Anderson B.J, & Kritzer M.F. (2020). Biological Sex and Sex Hormone Impacts on Deficits in Episodic-Like Memory in a Rat Model of Early, Pre-motor Stages of Parkinson's Disease. Frontiers in Neurology, 11, 942.

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