Recombinant human TNFα was from Beyotime Biotechnology. MG132 and cycloheximide (CHX) were from sigma. The cell lysates for immunoblotting analysis used antibodies against rabbit Cullin3 (Cell Signaling Technology, United States), mouse β-actin (Cwbiotech, China), mouse RhoB (Santa Cruz, United States), rabbit Ubiquitin (Cell Signaling Technology, United States), mouse TNFAIP1 (Santa Cruz, United States), rabbit KCTD10 (Proteintech, United States), mouse Flag (Abmart, China), rabbit Flag (Huabio, China), rabbit Myc (Huabio, China), rabbit HA (Huabio, China), rabbit p-p38 (Cell Signaling Technology, United States), rabbit p38 (Cell Signaling Technology, United States), rabbit p-JNK (Cell Signaling Technology, United States), and rabbit JNK (Cell Signaling Technology, United States). Densitometric analysis of the band intensities was performed using ImageJ.
Rabbit ubiquitin
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit Ubiquitin is a protein that plays a central role in the ubiquitin-proteasome system, which is responsible for the degradation of damaged or unwanted proteins within cells. It functions as a post-translational modification, tagging target proteins for destruction by the proteasome.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit p p38, by Cell Signaling Technology (1 mentions)
Mg132, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mouse β actin, by Cell Signaling Technology (1 mentions)
Normal rabbit igg, by Cell Signaling Technology (1 mentions)
Il 18, by Abcam (1 mentions)
Alexa fluor 488 donkey anti mouse, by Thermo Fisher Scientific (1 mentions)
Rabbit cx43, by Cell Signaling Technology (1 mentions)
Interleukin 2 (il 2), by Abcam (1 mentions)
Lucifer yellow, by Merck Group (1 mentions)
Protein a g plus agarose, by Santa Cruz Biotechnology (1 mentions)
Hoechst 33342, by Dojindo Laboratories (1 mentions)
Interleukin 6 (il 6), by Abcam (1 mentions)
2 protocols using rabbit ubiquitin
1
Regulation of RhoB ubiquitination
2
Rg1 Bioactivity and Signaling Pathway
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Rg1 (PubChem CID: 441923, C42H72O14, ≥ 98%) was purchased from Jilin University (Changchun, Jilin Province, China). Lucifer Yellow was obtained from Sigma-Aldrich (St. Louis, MO, United States). High-glucose Dulbecco’s Modified Eagle Media (DMEM) and fetal bovine serum were produced by Gibco (Grand Island, NY, United States). Additional antibody sources: mouse β-actin, normal rabbit IgG, rabbit Cx43, mouse Cx43, rabbit ubiquitin, and mouse ubiquitin antibodies were obtained from Cell Signaling Technology (Beverly, MA, United States); Alexa Fluor-488 donkey anti-mouse and 546 donkey anti-rabbit secondary antibodies were produced by Invitrogen (Carlsbad, CA, United States). Protein A/G Plus-Agarose was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Hoechst 33342 was produced by Dojindo Laboratories (Kumamoto, Japan). Rat and cell supernatant enzyme-linked immunosorbent assay (ELISA) Kits for interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), caspase-1, IL-2, IL-6, and IL-18 were purchased from Abcam (Cambridge, United Kingdom).
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