The International Biological Material Research Center of the Korea Research Institutes of Bioscience and Biotechnology (Daejeon, Republic of Korea) provided a lyophilized sample of MED (FBM 213-075). Briefly, dried stem powder of D. tuberculatus was mixed with methanol in a ratio of 1:10 (wt/vol). The mixture was sonicated for 15 min and incubated for 2 h at room temperature. The process was repeated 10 times daily for 3 days and filtered using a 0.4 µm filter. The extracted solution was concentrated using a rotary evaporator (N = 1000 SWD, EYELA, Bohemia, NY, USA) and lyophilized using a speed vacuum concentrator (Modulspin 40, Biotron Co., Marysville, WA, USA). The MED obtained was dissolved in 1×PBS buffer to the required concentrations for administration to mice.
N 1000swd
Manufactured by Eyela
Sourced in Japan, United States
The N-1000SWD is a laboratory equipment product. It is a compact and versatile device designed for various scientific applications. The core function of the N-1000SWD is to provide a controlled and consistent environment for a range of laboratory processes.
Automatically generated - may contain errors
21 protocols using n 1000swd
1
Extraction and Preparation of MED from D. tuberculatus
2
Extraction and Characterization of Cumin Seed
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C. cyminum L. was collected by Md. Salah Uddin from the Shibgonj sub-district, Bogra district, Rajshahi division in Bangladesh. Voucher samples were deposited in the herbarium of the Korea Research Institute of Bioscience and Biotechnology as KRIB 0086021. After drying and grinding the seeds of C. cyminum, the powder (75 g) was extracted by applying 1 L of 99.9% (v/v) methanol for 3 days at 45 °C. Sonication was performed for 15 min and resting was done for 2 h. The resultants were filtered with non-fluorescent cotton and concentrated at 45 °C by a rotary evaporator (N-1000SWD, EYELA, Tokyo, Japan) using reduced pressure. A total 13.16 g of CCT extract was obtained after freeze-drying procedures.
3
Extraction of Croton hirtus L'Hér. Medicinal Plant
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Croton hirtus L'Hér. was collected in the Na Xay village, Xayboury district, Savannakhet province in Laos, having been identified there by Sangwoo Lee (Korea Research Institute of Bioscience and Biotechnology, Republic of Korea) in 2010. A voucher specimen (accession number KRIB 0033811) of the retained material is preserved at the herbarium of KRIBB. Dried and refined Croton hirtus L'Hér whole plant (71 g) was extracted with 1 L of 99.9% (v/v) methanol via repeated cycles of sonication (15 min) and rest (2 h) for 3 days at 45°C. The resultant product was filtered with non-fluorescent cotton and concentrated by rotary evaporator (N-1000SWD; EYELA, Japan) under reduced pressure at 45°C. After freeze drying, a total of 4.44 g of Croton hirtus methanol extract remained.
4
Preparation of Danggui-Shaoyao-San Extract
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DKT extract powder was manufactured by Tsumura and Co., Tokyo. To prepare DKT constituents, the powders obtained from the ethanol or water extracts of Ginseng radix (Catalog number: CA03-041), Zingiberis rhizome (Catalog number: CA04-001), and Zanthoxyli fructus (Catalog number: 029-037) were obtained from the plant extract bank at the Korean Research Institute of Bioscience and Biotechnology (Daejeon, Korea). DKT and its constituents were authenticated by Hyungwoo Kim (Division of Pharmacology, Pusan National University, School of Korean Medicine, Yangsan, Korea). The powder was then immersed in ethanol or water, sonicated for 15 min, and extracted for 72 h. The extract so obtained was filtered through nonfluorescent cotton and evaporated under reduced pressure using a rotary evaporator (N-1000 SWD, Eyela, Japan) in 45°C. The condensed extract was then lyophilized using a Modul Spin 40 dryer (Biotron Corporation, Calgary, Canada) for 24 h. The yield of lyophilized powder obtained was 12.3%. The DKT was dissolved in distilled water at a concentration of 0.5 g (crude drug)/ml and stored in a refrigerator. The extracts of Ginseng radix, Zingiberis rhizome, and Zanthoxyli fructus were dissolved in dimethyl sulfoxide as a stock solution at 100 mg/mL and stored at 4°C. The stock solution was diluted with medium to the desired concentration prior to use.
5
Methanol Extract of Pterospermum elegans
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol extract of P. elegans stem was supplied by Foreign Plant Extract Bank (no. FBM118-023; Daejeon, Korea). The plant was collected by Sydara K (one of the authors of the paper) in Ham Ao, Laos in 2010 and authenticated by Institute of Traditional Medicine (ITM). A voucher specimen recoded as ‘Korea Research Institute of Bioscience and Biotechnology (KRIBB) 0033580ʹ was deposited in the herbarium of the Korea KRIBB. Briefly, the dried and refined aerial parts of P. elegans (92g) were extracted with 1,000 mL of 99.9% (v/v) methanol using a sonicator (SDN-900H, SD Ultrasonic Cleaner, Seoul, Korea) at 45°C for 3 days (15 min sonication at 1500 W and 40 kHz followed by 2 h standing; repeated 10 times per day). The resultant product was filtered with non-fluorescent cotton, condensed using a rotary evaporator (N-1000SWD, EYELA, Tokyo, Japan) under reduced pressure at 45°C, and lyophilized using a freeze dryer (Christ, Germany). Dimethyl sulfoxide (DMSO) was used as a solvent for lyophilized substances.
6
Extraction and Analysis of Angiopteris cochinchinensis Leaves
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An ethanol extract of the leaves of Angiopteris cochinchinensis de Vriese (Ac-EE) was received from the National Institute for Biological Resources (Incheon, Korea) belonging to the Ministry of Environment. The leaves of A. cochinchinensis were ground and dried before infiltration of 70% EtOH for 1 day at RT. The extract was then filtered in vacuum state at 40 °C under 10 hPa. After further enrichment with a rotary flash evaporator (N-1000SWD, EYELA), the extract was lyophilized. Prepared extract was resolved in DMSO to make 100 mg/mL stock solution for in vitro experiments. The stock was diluted with each cell culture medium for treatment. In the animal experiments, Ac-EE was dissolved in PBS to prepare the target concentration (100 mg/kg). Gas chromatography–mass spectrometry (GC-MS) analysis was introduced with the help of the Cooperative Center for Research Facilities in SKKU (Suwon, Republic of Korea).
7
Extraction of Malus baccata Phytochemicals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Malus baccata (L.) Borkh. was collected from Xiao Longman National Forest Park, Mentougou district, Beijing, China, and identified by Dr. Zhiyun Zhang at the Institute of Botany in May 2012. A voucher specimen (accession number KRIB 0041120) of the retained material is preserved at the herbarium of KRIBB. The leaves and shoots of Malus baccata (19 g) were extracted with 1 L of 99.9% (v/v) methanol under repeated sonication (15 min) and rest (2 h) for 3 days at 45 °C. The resultant product was filtered with non-fluorescence cottons and concentrated by a rotary evaporator (N-1000SWD, EYELA) under reduced pressure at 45 °C. Finally, a total 4.09 g of methanol extract of Malus baccata was obtained by freeze-drying.
8
Cannabinoid Receptor Assay and Antioxidant Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plant samples were first dried, crushed, and sieved through a no. 60 mesh. Then, the dried sample powder (1.0 g) was ultrasonically extracted with 70% methanol–water mixed solvent (100 mL) for 30 min. Obtained supernatant after centrifugation was filtered and stored at 4°C for subsequent UPLC-Q-TOF-MS and UPLC-DAD analyses.
To evaluate the antioxidant and agonistic activities of cannabinoid receptor CB1/CB2, the powdered samples (0.5 g) were ultrasonically extracted three times (20 W, 40 kHz) with 50 mL of a mixed solvent of methanol and water (70:30 v/v), each for 30 min. The solutions were collected and filtered with qualitative filter paper and concentrated using a rotary evaporator (N-1000SWD, Eyela, Tokyo, Japan). Afterward, the crude extraction was freeze-dried in a freeze dryer under the following conditions: the temperature of the cold trap was −50°C, and the pressure was 0.3 mbar (Lyovapor L-300, BUCHI, Flawil, Switzerland). Finally, the obtained crude extraction powder was hermetically sealed and stored in opaque glass bottles at 4°C until further use. For the assessment of antioxidant and CB1/CB2 receptor agonistic activities, 10 mg of each sample extraction powder was dissolved in 5 mL by 70% methanol–water mixed solvent and 1 mL by dimethyl sulfoxide (DMSO).
To evaluate the antioxidant and agonistic activities of cannabinoid receptor CB1/CB2, the powdered samples (0.5 g) were ultrasonically extracted three times (20 W, 40 kHz) with 50 mL of a mixed solvent of methanol and water (70:30 v/v), each for 30 min. The solutions were collected and filtered with qualitative filter paper and concentrated using a rotary evaporator (N-1000SWD, Eyela, Tokyo, Japan). Afterward, the crude extraction was freeze-dried in a freeze dryer under the following conditions: the temperature of the cold trap was −50°C, and the pressure was 0.3 mbar (Lyovapor L-300, BUCHI, Flawil, Switzerland). Finally, the obtained crude extraction powder was hermetically sealed and stored in opaque glass bottles at 4°C until further use. For the assessment of antioxidant and CB1/CB2 receptor agonistic activities, 10 mg of each sample extraction powder was dissolved in 5 mL by 70% methanol–water mixed solvent and 1 mL by dimethyl sulfoxide (DMSO).
9
Methanol Extraction of Guettarda crispiflora Leaves
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Guettarda crispiflora Vahl originally was collected in the San Juan de Punchis district, Zamora Chinchipe province in Ecuador, and was identified by Bolivar Merino, the herbarium of Loja National University in 2012 March. A voucher specimen (accession number KRIB 0044435) of the retained material is preserved at the herbarium of KRIBB (Daejeon, Korea).
The leaves of G. crispiflora were extracted with 1 L of 99.9% (v/v) methanol through repeat sonication and rest for 3 days at 45 °C. The resultant product was filtered with non-fluorescence cotton and concentrated using a rotary evaporator (N-1000SWD, EYELA) under reduced pressure at 45 °C, as reported previously [45 (link)]. Finally, a total of 2.15 g of methanol extract of G. crispiflora was obtained by freeze-drying.
The leaves of G. crispiflora were extracted with 1 L of 99.9% (v/v) methanol through repeat sonication and rest for 3 days at 45 °C. The resultant product was filtered with non-fluorescence cotton and concentrated using a rotary evaporator (N-1000SWD, EYELA) under reduced pressure at 45 °C, as reported previously [45 (link)]. Finally, a total of 2.15 g of methanol extract of G. crispiflora was obtained by freeze-drying.
10
Extraction of Ulmus davidiana
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
U. davidiana var. japonica was purchased at the herbal medicine market in Seoul, South Korea. 1 kg was extracted with 10 L of 70% ethanol for 24 hours at room temperature (RT). The ethanol extraction supernatant was filtered through filter paper (Whatman No. 1 & 3; Whatman, UK) and separated using a rotary vacuum vaporizer (N-1000S-WD; Eyela Co., Japan). The yield (w/w) obtained with UDE was 12.4%.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!