Jsm 5400

Manufactured by JEOL

Sourced in Japan

The JSM-5400 is a scanning electron microscope (SEM) manufactured by JEOL. It is designed for high-resolution imaging and microanalysis of a wide range of materials. The instrument features a tungsten filament as the electron source and provides a magnification range from 8x to 300,000x.

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Lab products found in correlation

Jfc 1100, by JEOL (2 mentions) Malvern zetasizer nano z, by Malvern Panalytical (2 mentions) Jec 550, by JEOL (1 mentions) Jfc 1100e, by JEOL (1 mentions) Jsm 6700f, by JEOL (1 mentions) Axiostar light microscope, by Zeiss (1 mentions) Jfc 1199e, by JEOL (1 mentions) D8 advance, by Bruker (1 mentions) Thermal analyzer, by TA Instruments (1 mentions) Hexamethyldisilazane, by Merck Group (1 mentions) Bx60m, by Olympus (1 mentions) Zetasizer software, by Malvern Panalytical (1 mentions) Zetasizer nano zs instrument, by Malvern Panalytical (1 mentions)

35 protocols using jsm 5400

Characterization of Electrodes via SEM, EDS, XRD

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SEM was performed using a JEOL JSM 5400 scanning microscope after coating them with gold using a JEOL JFC-1199E ion sputtering device for the characterization of L.C and without coating for the characterization of the SS/PbO2 anodes used.
EDS was planned to assess the surface elemental compositions of L.C using a JEOL JSM 5400 scanning microscope. EDS was performed after coating them with gold using a JEOL JFC-1199E ion sputtering device.
XRD characterizations of the elaborated SS/PbO2 anodes were done using an X-ray diffractometer D8 Advance (Bruker, (CNRSM)) and a Diano, Ka Co radiation at a wavelength of 1.54 nm using the Debye method (Holzwarth and Gibson 2011) (link).

Kollati P.R, & Mohapatra S.S. (2021). The combined chemical and mechanical modifications of cigarette: a novel methodology to reduce harmful effects. Environmental science and pollution research international, 28(47).

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Fabric Surface Characterization and Nanoparticle Analysis

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The surface characteristics of the treated fabrics and their metal nanoparticle contents were evaluated using SEM (JSM-5400, JEOL, Tokyo, Japan) coupled with energy-dispersive X-ray spectroscopy (EDX).

Salem S.S., EL-Belely E.F., Niedbała G., Alnoman M.M., Hassan S.E., Eid A.M., Shaheen T.I., Elkelish A, & Fouda A. (2020). Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics. Nanomaterials, 10(10), 2082.

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Fabric Characterization via EDX-SEM

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Energy dispersive X-ray spectroscopy (EDX) in combination with SEM (Jeol–JSM-5400, Japan) was used to determine the morphological and surface characterization as well as the elemental composition of the treated fabrics.

Eid A.M., Fouda A., Niedbała G., Hassan S.E., Salem S.S., Abdo A.M., F. Hetta H, & Shaheen T.I. (2020). Endophytic Streptomyces laurentii Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties. Antibiotics, 9(10), 641.

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Enamel Surface Examination via SEM

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Five teeth from each group were randomly selected to examine morphological changes on the enamel surface via SEM, while the other 20 teeth from each group underwent bracket bonding (JEOL JSM 5400, Tokyo, Japan).

Sallam R.A, & Arnout E.A. (2018). Effect of Er: YAG laser etching on shear bond strength of orthodontic bracket. Saudi Medical Journal, 39(9), 922-927.

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Isolation and Characterization of Irradiation-Resistant Bacteria

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For the isolation of IRRB, the dose of 10 kGy was chosen and 1 g of irradiated roots of P. turgidum was crushed as indicated in the previous step. After shaking in an orbital shaker, at 150 rpm for 30 min at room temperature, each mixture was serially diluted (10⁻¹–10⁻⁵). A volume of 100 μL of each dilution was plated on TSA. After 7 days of incubation at 30 °C, different morphotypes were selected, purified, maintained in tryptic soy broth (TSB) and stored in 25% glycerol (vol/vol) at − 80 °C (Guesmi et al., 2019 ).
Morphological features of most IRRB were examined using a Scanning Electron Microscope (JSM 5400, JEOL, Japan) as described by Kaewkla and Franco (2019) (link). After growth on TSA plates at 30 °C for 48 h, bacterial cells were fixed with 2% w/vol glutaraldehyde in 0.9% NaCl solution and then fixed cells were dried with graded (50, 70, 90 and 100%) ethanol solutions. All manipulations were done in triplicates (3).

Guesmi S., Najjari A., Pujic P., Ghedira K., Ouertani R., Jabberi M., Cherif A., Normand P, & Sghaier H. (2021). Roots of the xerophyte Panicum turgidum host a cohort of ionizing-radiation-resistant biotechnologically-valuable bacteria. Saudi Journal of Biological Sciences, 29(2), 1260-1268.

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Scanning Electron Microscopy for Mineral Analysis

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Scanning electron microscopy was performed on the samples selected from optical microscopy (NS1, NS3, NS8) using a Jeol JSM 5400 to determine the chemical composition of mineral phases through EDS of backscattering (BSE) images. To produce an electrically conductive surface for SEM, both thin and cross sections were coated using thin-film evaporation of graphite in a vacuum coater, with a thin layer of about 20 nm thickness.

Reale R., Andreozzi G.B., Sammartino M.P, & Salvi A.M. (2023). Analytical Investigation of Iron-Based Stains on Carbonate Stones: Rust Formation, Diffusion Mechanisms, and Speciation. Molecules, 28(4), 1582.

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Scaffold Microstructure Analysis

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The micro- and macrostructures of the scaffolds were analyzed by scanning electron microscopy (SEM) and optical microscopy, respectively. Scaffolds were dehydrated with graded ethanol, air-dried, and sputter-coated with gold for 80 sec at 40 mA (Sputter Coating Device SCD 005, Bal-Tec AG, Liechtenstein). Analysis was performed at 5 kV accelerating voltage in a scanning electron microscope (Jeol JSM-5400, Japan). For macroanalysis, scaffolds were imaged using a stereoscope (Zeiss, Jena, Germany) from the side and top view.

Wahl E.A., Fierro F.A., Peavy T.R., Hopfner U., Dye J.F., Machens H.G., Egaña J.T, & Schenck T.L. (2015). In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds. BioMed Research International, 2015, 108571.

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Ultrastructural Analysis of Maxillae in TRPV4 Mice

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Maxillae from TRPV4^+/+ and TRPV4^−/− mice (n = 3 per group) were fixed with 2.5% glutaraldehyde in 0.1 mol/L sodium cacodylate buffer overnight and decalcified with 10% EDTA. The gingival tissues were carefully separated from the teeth and postfixed with 1% osmium tetroxide for 1 hour at 4°C. The tissues were dehydrated in a graded ethanol series, immersed in t‐butyl alcohol, freeze‐dried, sputter‐coated with platinum using an ion coater (JEC‐550; JEOL) and examined under a scanning electron microscope (JSM‐5400; JEOL).

Kitsuki T., Yoshimoto R.U., Aijima R., Hatakeyama J., Cao A., Zhang J., Ohsaki Y., Mori Y, & Kido M.A. (2019). Enhanced junctional epithelial permeability in TRPV4‐deficient mice. Journal of Periodontal Research, 55(1), 51-60.

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Comprehensive Mineralogical Characterization

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The mineralogical composition of the fraction of the LRA (de Vera et al., 2019 (link)) used in this study was further characterized by XRF, XRD and SEM‐EDS. The total chemical composition of the major elements (wt.% oxides) was obtained using a Rigaku Supermini X‐ray fluorescence spectrometer, with natural and synthetic standards, and an uncertainty of 0.01% (for sample preparation and analytical details, see Tangari et al. (2020 )).
XRD measurements of the powdered samples were obtained using a Rigaku MiniFlex II Benchtop XRD System (Rigaku Company, TX, USA) operating at a voltage of 20 kV and a current of 15 mA with CuK_α radiation. The mineral phase was identified by comparing the calculated values of interplanar spacing and corresponding intensities of diffraction peaks with theoretical values from the Powder Diffraction File database (JCPDS‐ICDD 2008). The target material of the X‐ray tube was Pd. SEM‐EDS analyses were performed using a JEOL JSM 5400 with an EDS attachment (iXRF Si‐drift detector with ultrathin window) (acc. voltage: 20 keV; carbon‐coated samples).

Cassaro A., Pacelli C., Baqué M., Cavalazzi B., Gasparotto G., Saladino R., Botta L., Böttger U., Rabbow E., de Vera J, & Onofri S. (2022). Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions. Environmental Microbiology, 24(7), 2938-2950.

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Dehydrating and Sputter-Coating Teeth Samples

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Teeth samples were dehydrated in ethanol solutions of increasing gradients (50%, 70%, 80%, 90% and 100%). Each bath required 5 min (Fig. 1-F). Samples were conserved in ethanol 100% at 4 °C untel be used (Fig. 1-G). These samples were sputter-coated with gold using a JEOL (JFC-1100E) coater (Fig. 1-H) and then dried under vaccum and observed using high-resolution SEM (JEOL, JSM-5400) at 5000X and 10000X magnifications.

Kammoun R., Zmantar T, & Ghoul S. (2020). Scanning electron microscopy approach to observe bacterial adhesion to dental surfaces. MethodsX, 7, 101107.

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