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Signalfire elite ecl reagent

Manufactured by LI COR
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SignalFire™ Elite ECL Reagent is a chemiluminescent substrate designed for the detection of horseradish peroxidase (HRP) in Western blotting applications. It provides a sensitive and stable signal for the visualization of target proteins.

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Lab products found in correlation

Sigmafast bcip nbt, by LI COR (3 mentions) C digit chemiluminescent blot scanner, by LI COR (3 mentions) Trans blot turbo transfer system, by Bio-Rad (3 mentions) Monoclonal murine anti polyhistidine antibody, by Merck Group (2 mentions) Phospho threonine antibody, by Cell Signaling Technology (2 mentions) Mouse anti rabbit igg antibody alkaline phosphatase, by Merck Group (2 mentions) Anti mouse igg whole molecule alkaline phosphatase antibody, by Merck Group (2 mentions) Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

ECL reagent

3 protocols using signalfire elite ecl reagent

1

Immunoblotting of Mycobacterial Proteins

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Proteins were separated on 4–20% gradient SERVA gels and transferred onto a nitrocellulose membrane using a Trans‐Blot® Turbo™ Transfer System (Bio‐Rad). SIGMAFAST™ BCIP®/NBT or SignalFire™ Elite ECL Reagent were used to visualise proteins on C‐DiGit Chemiluminescent Blot Scanner (LI‐COR Biosciences). The following antibodies were used: custom polyclonal antibody raised against Lsr2 in rabbit (Gemini Biosciences); monoclonal murine anti‐polyhistidine antibody (Sigma‐Aldrich); phospho‐threonine antibody (Cell Signaling Technology); monoclonal anti‐MtbGroEL2 (Rv0440), clone IT‐70 (BEIResources); mouse anti‐rabbit IgG antibody:alkaline phosphatase (Sigma‐Aldrich; anti‐mouse IgG (whole molecule:alkaline phosphatase antibody produced in rabbit (Sigma‐Aldrich), and anti‐rabbit IgG, HRP‐linked antibody (Cell Signaling Technology).
Alqaseer K., Turapov O., Barthe P., Jagatia H., De Visch A., Roumestand C., Wegrzyn M., Bartek I.L., Voskuil M.I., O'Hare H.M., Ajuh P., Bottrill A.R., Witney A.A., Cohen‐Gonsaud M., Waddell S.J, & Mukamolova G.V. (2019). Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112. Molecular Microbiology, 112(6), 1847-1862.
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2

Western Blot Analysis of Lsr2 Protein

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Proteins were separated on 4–20% gradient SERVA gels and transferred onto a nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (Bio-Rad). SIGMAFAST™ BCIP®/NBT or SignalFire™ Elite ECL Reagent were used to visualise proteins on C-DiGit Chemiluminescent Blot Scanner (LI-COR Biosciences). The following antibodies were used: custom polyclonal antibody raised against Lsr2 in rabbit (Gemini Biosciences); monoclonal murine anti-polyhistidine antibody (Sigma-Aldrich); phospho-threonine antibody (Cell Signaling Technology); monoclonal anti-MtbGroEL2 (Rv0440), clone IT-70 (BEIResources); mouse anti-rabbit IgG antibody:alkaline phosphatase (Sigma-Aldrich; anti-mouse IgG (whole molecule:alkaline phosphatase antibody produced in rabbit (Sigma-Aldrich), and anti-rabbit IgG, HRP-linked antibody (Cell Signaling Technology).
Alqaseer K., Turapov O., Barthe P., Jagatia H., De Visch A., Roumestand C., Wegrzyn M., Bartek I.L., Voskuil M.I., O'Hare H.M., Ajuh P., Bottrill A.R., Witney A.A., Cohen‐Gonsaud M., Waddell S.J, & Mukamolova G.V. (2019). Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112. Molecular Microbiology, 112(6), 1847-1862.
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3

Protein Separation and Visualization

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Proteins were separated on 4%–20% gradient SERVA gels and transferred onto a nitrocellulose membrane using a Trans-Blot® Turbo Transfer System (Bio-Rad). SIGMAFAST BCIP®/NBT or SignalFire Elite ECL Reagent were used to visualize proteins on C-DiGit Chemiluminescent Blot Scanner (LI-COR Biosciences), according to the manufacturer’s instructions.
Turapov O., Forti F., Kadhim B., Ghisotti D., Sassine J., Straatman-Iwanowska A., Bottrill A.R., Moynihan P.J., Wallis R., Barthe P., Cohen-Gonsaud M., Ajuh P., Vollmer W, & Mukamolova G.V. (2018). Two Faces of CwlM, an Essential PknB Substrate, in Mycobacterium tuberculosis. Cell Reports, 25(1), 57-67.e5.
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