LC–MS grade water, LC–MS grade acetonitrile were purchased from VWR international (UK). LC–MS grade methanol, N,O-bis-(trimethylsilyl)trifluoro-acetamide (BSTFA) with 1% trimethylchlorosilane (TMCS), O-methoxyamine-HCl (MOX), succinic-d4 acid (98% purity), itaconic acid (96%) were purchased from Sigma-Aldrich (UK). Ciprofloxacin (batch number: 1130026) was purchased from Guangzhou Bai Yunshan Pharmaceutical General Factory (China), Relinqing® granule (batch number: 141005) was purchased from Guizhou Wei Men Pharmaceutical Company.
Lc ms grade water
Manufactured by Avantor
Sourced in United States, Germany, United Kingdom, Ireland, Belgium
LC-MS grade water is a high-purity water used in liquid chromatography-mass spectrometry (LC-MS) applications. It is designed to meet the stringent requirements of LC-MS analysis, ensuring minimal interference and maximum sensitivity.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Avantor (14 mentions)
Acetonitrile, by Avantor (10 mentions)
Lc ms grade acetonitrile, by Avantor (5 mentions)
Formic acid, by Merck Group (5 mentions)
Formic acid, by Avantor (5 mentions)
Methanol, by Thermo Fisher Scientific (4 mentions)
Formic acid, by Thermo Fisher Scientific (4 mentions)
Lc ms grade methanol, by Merck Group (3 mentions)
Acetonitrile, by Merck Group (3 mentions)
Urea, by Merck Group (3 mentions)
Ammonium bicarbonate, by Avantor (3 mentions)
Sodium hydroxide (naoh), by Merck Group (3 mentions)
D glucose, by Merck Group (3 mentions)
Hydrofluoric acid, by Thermo Fisher Scientific (3 mentions)
Methanol, by Honeywell (2 mentions)
Lc ms grade acetonitrile acn, by Honeywell (2 mentions)
Ammonium formate, by Avantor (2 mentions)
Thc d3, by Cerilliant (2 mentions)
Cbn d3, by Cerilliant (2 mentions)
Cbd d3, by Cerilliant (2 mentions)
55 protocols using lc ms grade water
1
Quantitative Analysis of Bioactive Compounds
2
Comprehensive Metabolite Profiling Protocol
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LC−MS grade methanol (MeOH), acetonitrile (ACN), 2-propanol (IPA), formic acid (FA), and butylated hydroxytoluene (BHT, 99%) were purchased from Sigma- Aldrich (Taufkirchen, Germany). LC−MS grade water; HPLC-grade n-hexane, ethyl acetate, and acetone were purchased from VWR International, LLC. (Pennsylvania, US). DIAL standards including all- trans-2,7-dimethylocta-2,4,6-trienedial (4, Figure 1 ), all-trans-2,6-dimethylocta-2,4,6-trienedial (5), all-trans-2,6,11-trimethyldodeca- 2,4,6,8,10-pentaenedial (8), and all-trans-2,6,11,15-tetramethylhexa- deca-2,4,6,8,10,12,14-heptaenedial (11) and apocarotenoid standards including all-trans-3-OH-β-apo-11-carotenal (OH-Apo11), all-trans- 3-OH-β-apo-15-carotenal (OH-Apo15), and all-trans-3-OH-β-apo-12′-carotenal (OH-Apo12′) were obtained from Buchem B.V. (Apeldoorn, Netherlands). N2,N2,N4,N4-Tetraethyl-6-hydrazinyl- 1,3,5-triazine-2,4-diamine (T3) was bought from Chemspace. Silica gel SPE columns (500 mg/3 mL) were purchased from Thermo Scientific. D6-all-trans-2,7-Dimethylocta-2,4,6-trienedial (D6-4) was synthesized by Dr. Magnus Rueping according to the protocol.13 (link) Several vegetables including tomato, carrot, yellow pepper, spinach, and sweet potato were obtained from the supermarket.
3
Metabolomics Analysis of Traditional Chinese Medicine
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LC-MS grade water, LC-MS grade acetonitrile were purchased from VWR international (UK). LC-MS grade methanol, N,O-bis(trimethylsilyl)trifluoro-acetamide (BSTFA) with 1% trimethylchlorosilane (TMCS), O-methoxyamine-HCL (MOX), succinic-d4 acid were purchased from Sigma-Aldrich (UK). Ciprofloxacin (batch number: 1130026) was purchased from Guangzhou Bai Yun Shan Pharmaceutical General Factory (China). Relinqing ® granules (batch number: 141005) was purchased from Guizhou Wei Men Pharmaceutical Company.
4
Quantitative Proteomic Sample Preparation
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LC-MS-grade water was purchased from VWR International (Radnor, PA, USA). Reagents for TMT10plex, the MicroBCA protein assay, and Pierce Concentrator 3KDa MWCO 0.5 mL were purchased from Thermo Fisher Scientific (Rockford, IL, USA). The protease inhibitors cocktail cOmpleteTM Mini EDTA-free EASYpack was acquired from Roche (Basel, Switzerland). Lysyl endopeptidase C (Lys-C), mass spectrometry grade, was bought from Wako (Neuss, Germany).
Furthermore, the 0.22 µm spin filters, AssayMap BRAVO 5 µL cartridges (C18 and RPS), and immunodepletion Multiple Affinity Removal Spin Cartridge MOUSE-3 were purchased from Agilent Technologies (Santa Clara, CA, USA).
Vivaspin 500 3 kDa MWCO filters were bought from Sartorius (Gottingen, Germany). Trypsin/Lys-C mix Mass Spec grade was purchased from Promega (Madison, WI, USA), and size Exclusion Chromatography columns (qEV-70 nm) were obtained from IZON (Christchurch, NZ, USA).
All other reagents were purchased from Sigma-Aldrich (Saint Louis, MO, USA).
Furthermore, the 0.22 µm spin filters, AssayMap BRAVO 5 µL cartridges (C18 and RPS), and immunodepletion Multiple Affinity Removal Spin Cartridge MOUSE-3 were purchased from Agilent Technologies (Santa Clara, CA, USA).
Vivaspin 500 3 kDa MWCO filters were bought from Sartorius (Gottingen, Germany). Trypsin/Lys-C mix Mass Spec grade was purchased from Promega (Madison, WI, USA), and size Exclusion Chromatography columns (qEV-70 nm) were obtained from IZON (Christchurch, NZ, USA).
All other reagents were purchased from Sigma-Aldrich (Saint Louis, MO, USA).
5
Quantification of Prostaglandin Biomarkers
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Citric acid, phosphoric acid, L(+)-ascorbic acid, 1-butanol, and ethyl acetate were from Roth (Karlsruhe, Germany). Oxidized LDL ELISA kits (Cod: 10-1143-01) were from Mercodia (Uppsala, Sweden). The 9α,11α,15S-trihydroxy-prosta-5Z,13E-dien-1-oic-3,3,4,4-d4acid (PGF2α-d4, CAS 34210-11-2), 9β,11β,15R-trihydroxy-(8β,12α)-prosta-5Z,13E-dien-1-oic acid (ent-PGF2α, CAS 54483-31-7), 9α,11α,15S-trihydroxy-(8β)-prosta-5Z,13E-dien-1-oic acid (8-iso PGF2α, CAS 27415-26-5), and (3Z)-5-[(1S,2R,3R,5S)-3,5-dihydroxy-2-[(1E,3S)-3-hydroxy-1-octen-1-yl]cyclopentyl]-3-pentenoic acid (2,3-dinor-8-isoPGF2α, CAS 221664-05-7) were purchased from Cayman Chemical (Ann Arbor, MI, USA). Formic acid was from Merck (Darmstadt, Germany). LC-MS-grade water and methanol were purchased from VWR Chemicals (Darmstadt, Germany).
6
Quantitative Proteomic Sample Preparation
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Acetonitrile and formic acid were purchased from Biosolve Chemicals (Dieuze, France). Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) and cysteamine were provided by Sigma-Aldrich (St. Louis, MO, USA). LC-MS-grade water was purchased from VWR International (Radnor, PA, USA). cysteamine-D4 hydrochloride was purchased from LGC (Middlesex, UK).
7
HPLC-grade Organic Solvents Acquisition
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HPLC-grade acetone, methanol and acetonitrile (ACN) as well as absolute ethanol (99.8%) were purchased at Fisher Chemical (Loughborough, UK). HPLC grade formic acid was obtained from Carl Roth GmbH + Co. KG (Karlsruhe, Germany) and LC–MS grade water was from VWR (Vienna, Austria).
8
SILuMAb Antibody Characterization Protocol
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SILuMAb universal antibody standard human (MSQC4) was purchased from Merck, Darmstadt, Germany. DEPC was obtained from Acros Organics, Geel, Belgium (Product no. 10114380). Imidazole (Product no. I2399), urea (Product no. U5378), ammonium bicarbonate (ABC; Product no. 09830), iodoacetamide (IAA) (Product no. I6125) and C18 disks- 47 mm (Product no. 66883-U) were purchased from Merck. Tris (2-carboxyethyl) phosphine (TCEP) was obtained from Alfa Aesar, Karlsruhe, Germany, and PNGaseF was purchased from Serva, Heidelberg, Germany. Sequencing grade modified trypsin (product no. V5111) was procured from Promega (Madison, WI, USA), formic acid (FA), and LC-MS grade water from VWR (Darmstadt, Germany). LC-MS grade acetonitrile (ACN) and methanol were purchased from Honeywell (Muskegon, MI, USA). Sodium chloride was obtained from Chemsolute, Renningen, Germany, and monobasic sodium phosphate from AppliChem, Darmstadt, Germany.
9
Purification and Formulation of Trimeric Env Proteins
10
Quantitative analysis of phytochemicals
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For the chemical analysis, HCl (analytical reagent grade) (a.r.), 37%, NaOH (≥97.0%) LC–MS grade water, and a 3kDa PES membrane filter were ordered from VWR International, Radnor, USA. The AccQ-Tag Ultra derivatization reagent kit, a mixture of standard proteinogenic amino acids, the AccQ-tag Ultra eluent A and AccQ-tag Ultra eluent B were purchased from Waters (Waters, Milford, MA, USA). The gradient grade methanol (MetOH) (≥99.9%) and standards of phytochemical compos: nicotinamide (≥98%), nicotinic acid (a. s.), biotin (≥99%), riboflavin (a. s.), liquiritigenin (≥97% (HPLC), formononetin (a. s.), chlorogenic acid (analytical standard), neochlorogenic acid (≥98%), chryptochlorogenic acid (a. s.), syringaldehyde (98%), sulforaphane (≥90% synthetic, liquid), sinapic acid (≥98%), scopoletin (analytical standard), apigenin (≥95.0%); apigenin-7-O-glucuronide (primary reference standard); luteolin (≥98%); quercetin (≥95.0%); isoquercitrin (a. s.); naringenin (≥95.0%); genkwanin (≥98%); kaempferol (≥97.0% (HPLC); isoliquiritigenin (a. s.), and ferulic acid (USP reference standard) were obtained from Sigma-Aldrich (Darmstadt, Germany).
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