Myometrial and decidual tissue from gd10.5 pregnant females was snap frozen on dry ice and the whole litter pooled. 150 mg tissue was added to 1 ml lysis buffer (150 mM NaCl, 20 mM Tris, 1 mM EGTA, 1% Triton X-100, 1/100 Phosphatase Inhibitor Cocktail 3 [Sigma], 1/100 Phosphatase Inhibitor II [Sigma], 2× Ultra Protease Inhibitor solution [Roche], and 2 mM PMSF) and processed with a tissue homogenizer on ice. The homogenate was incubated on ice with agitation for 30 min and spun at 20,000 × g for 10 min. The supernatant was used for further analysis on the MesoScale Ultra Sensitive platform. Total protein concentration was determined using a Bradford assay (Pierce).
Ultra sensitive platform
Manufactured by Mesoscale
The MesoScale Ultra Sensitive platform is a high-performance laboratory equipment designed for sensitive and quantitative detection of a wide range of analytes. It utilizes electrochemiluminescence technology to provide precise and reliable measurements. The core function of this platform is to enable researchers and scientists to conduct sensitive and accurate analyses in their respective fields of study.
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Lab products found in correlation
2 protocols using ultra sensitive platform
1
Extraction of Myometrial and Decidual Proteins
2
Quantification of Placental Proteins
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Tissue from pregnant females at gd10.5 were snap frozen. 150mg tissue from the antimesometrial side of implantation sites were added to 1mL lysis buffer and processed with a tissue homogenizer. After centrifugation of the homogenate, the supernatant was collected and analysed with MesoScale Ultra Sensitive platform as described previously (69 (link)). Total protein concentration was determined by Bradford assay (Pierce).
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