Qscript cdna supermix synthesis kit

Manufactured by Quanta Biosciences

The QScript™ cDNA SuperMix synthesis kit is a reagent used for the reverse transcription of RNA into complementary DNA (cDNA). The kit contains all the necessary components, including a reverse transcriptase enzyme, required for the cDNA synthesis reaction.

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Lab products found in correlation

Steponeplus real time pcr system, by Thermo Fisher Scientific (2 mentions) Powerup sybr green master mix, by Thermo Fisher Scientific (2 mentions) Maxima sybr green fluorescein master mix, by Thermo Fisher Scientific (1 mentions) Cfx96, by Bio-Rad (1 mentions) Stepone software v2, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

QScript cDNA Synthesis Supermix QScript cDNA Supermix kit QScript cDNA synthesis kit QScript cDNA SuperMix QScript cDNA synthesis CDNA Synthesis SuperMix QScript Synthesis Kit QScript cDNA kit QScript Supermix CDNA synthesis kit

2 protocols using qscript cdna supermix synthesis kit

Quantitative Real-Time PCR Analysis

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Between 0.7 and 1 μg RNA, extracted as above, was reverse-transcribed using qScript™ cDNA SuperMix synthesis kit (Quanta BioSciences, Inc., Gaithersburg, MD). Quantitative Real Time PCR was carried out in triplicates using a CFX96 instrument (Bio-Rad Laboratories, Inc., Hercules, CA), the Maxima SYBR Green/Fluorescein Master Mix (Thermo Fisher Scientific, Waltham, MA), and the primers listed in Supplemental Table S1. Relative mRNA expression values were normalized to those of either 18S RNA (ST2 cells) or Gapdh mRNA (NeMCO), which themselves varied minimally across samples.

MORIMOTO E., LI M., KHALID A.B., KRUM S.A., CHIMGE N.O, & FRENKEL B. (2016). Glucocorticoids Hijack Runx2 to Stimulate Wif1 for Suppression of Osteoblast Growth and Differentiation. Journal of cellular physiology, 232(1), 145-153.

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Quantitative Analysis of Placental Gene Expression

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RNA was reverse transcribed using qScript cDNA SuperMix synthesis kit (Quanta Biosciences) and subjected to qPCR (∆∆CT) analysis, using PowerUP SYBR Green Master Mix (Thermo Fisher Scientific) on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific) and forward and reverse primer sets for TEAD4 (F: 5'-CGGCACCATTACCTCCAACG-3'; R: 5'-CTGCGTCATTGTCGATGGGC-3'), ITGA6 (F: 5'-CACTCGGGAGGACAACGTGA-3'; R: 5'-ACAGCCCTCCCGTTCTGTTG-3'), CGB (F: 5'-GCCTCATCCTTGGCGCTAGA-3'; R: 5'-TATACCTCGGGGTTGTGGGG-3'), CSH1 (F: 5'-GACTGGGCAGATCCTCAAGCA-3'; R: 5'-CCATGCGCAGGAATGTCTCG-3'), ITGA5 (F: 5'-GGGTCGGGGGCTTCAACTTA-3'; R: 5'-ACACTGACCCCGTCTGTTCC-3'), HLA-G (F: 5'-CCCACGCACAGACTGACAGA-3'; R: 5'-AGGTCGCAGCCAATCATCCA-3'), NOTCH1 (F: 5'-GCCTGAATGGCGGGAAGTGT-3'; R: 5'-TAGTCTGCCACGCCTCTGC-3'), NOTCH2 (F: 5'-ACTCGGGGCCTACTCTGTGA-3'; R: 5'-TGTCTCCCTCACAACGCTCC-3'), GAPDH (F: 5'-AGGGCTGCTTTTAACTCTGGT-3' R: 5'-CCCCACTTGATTTTGGAGGGA-3'). All raw data were analyzed using StepOne Software v2.3 (Thermo Fisher Scientific). The threshold cycle values were used to calculate relative gene expression levels. Values were normalized to endogenous GAPDH transcripts.

Shannon M.J., Baltayeva J., Castellana B., Wächter J., Yoon S., Treissman J., Le H., Lavoie P.M., & Beristain A.G. (2021). Cell trajectory modeling identifies a primitive trophoblast state defined by BCAM enrichment.

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