The manure was diluted to 1 g per 9 ml of buffered peptone water (CM1049, Oxoid), and 10 µl of the suspended material was streaked on mCCDA and incubated as described above.
Cm1049
Manufactured by Thermo Fisher Scientific
Sourced in Denmark, United Kingdom
The CM1049 is a laboratory equipment designed for centrifugation. It features a compact and durable construction, making it suitable for a variety of laboratory applications. The product's core function is to separate and isolate samples through the process of centrifugation.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using cm1049
1
Isolation of Campylobacter from Manure
2
Salmonella Typhimurium Contamination Levels
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A Danish strain of Salmonella enterica serovar Typhimurium DT12 (S. Typhimurium or ST) previously made resistant to rifampicin (Jensen et al., 2006 (link)) was used to contaminate the substrate in an experimental laboratory study. A colony from an overnight (o.n.) plate-spread on tryptic soy agar with sheep blood (TSASB) (98763, SSI Diagnostica, Copenhagen, Denmark) was transferred into 10 mL Buffered Peptone Water (CM1049, Oxoid, DK) and incubated o.n. at 37°C. A 10-fold dilution series of the o.n. culture was prepared in 0.9% NaCl solution to reach expected contamination levels ranging from approximately 2 to 7 log CFU/g, and 0.1 mL of each dilution was plate-spread onto TSASB to determine the exact concentration of ST.
3
Fecal Microbiome Extraction from Socks
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Each pair of boot socks was placed in a stomacher bag, and after being diluted in 1:10 w/w in buffered peptone water (CM1049, Oxoid), faeces were released by gentle manipulation and 10 µl of the suspension was spread on mCCDA and incubated as described above.
4
Bacterial Strain Cultivation and Enumeration
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E. coli and S. Typhimurium strains supplied by the collection of the Experimental Zooprophylactic Institute of Southern Italy were included in this study. Bacterial strains were cultured in blood agar for 18 hours at 37°C and then activated in buffered peptone water (CM1049, OXOID, Basingstoke, UK) and incubated at 37°C for 18 hours. Subsequently, 10-fold serial dilutions of the culture were prepared in peptone water, and the cell concentration was measured by plating onto plate count agar (CM0325, OXOID, Basingstoke, UK) after incubation at 37°C for 24 hours.
5
Salmonella Screening in Egg Shells
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The sterile plastic bags containing selected eggs were open with scissors and the samples processed immediately. Swabs were used to sample the shell surface of the intact eggs. Sterile cotton swabs dipped into sterile buffered peptone water (Oxoid) (CM1049) was used to swab the entire surface area of the egg shell. The same eggs from which shell sample collected will be used for interior (egg contents) sampling. The egg's surface was sterilized using 70% ethanol followed by aming and then cracked open with a sterile scalpel blade. The isolation was conducted utilizing the conventional methods for the detection of Salmonella spp. following the standard guidelines from ISO 6579 [18] .
6
Buffered Peptone Water Preparation
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Buffered peptone water (BPW, Oxoid CM1049) was prepared according to the manufacturer's instructions. BPW-S was prepared by using BPW with the following supplements added before autoclaving: 40 µM 8-hydroxyquinoline, 0.5 g/l ammoniumiron(III) citrate, 0.1 g/l sodium deoxycholate, 0.1g /l sodium pyruvate (all from Sigma-Aldrich, Buchs, Switzerland). 6xBPW (quantities for 3xBPW in brackets) was prepared by addition of 7.5 g/l (3 g/l) KH 2 PO 4 and 17.5 g/l (7 g/l) Na 2 HPO 4 (anhydrous) (Sigma-Aldrich) to BPW. 6xBPW-S contains both additional buffer salts and supplements of BPW-S. Throughout this work, buffered peptone water from a single batch was used to avoid lot-to-lot variations.
7
Buffered Peptone Water Preparation
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Buffered peptone water (BPW, Oxoid CM1049) was prepared according to the manufacturer's instructions. BPW-S was prepared by using BPW with the following supplements added before autoclaving: 40 µM 8-hydroxyquinoline, 0.5 g/l ammoniumiron(III) citrate, 0.1 g/l sodium deoxycholate, 0.1g /l sodium pyruvate (all from Sigma-Aldrich, Buchs, Switzerland). 6xBPW (quantities for 3xBPW in brackets) was prepared by addition of 7.5 g/l (3 g/l) KH 2 PO 4 and 17.5 g/l (7 g/l) Na 2 HPO 4 (anhydrous) (Sigma-Aldrich) to BPW. 6xBPW-S contains both additional buffer salts and supplements of BPW-S. Throughout this work, buffered peptone water from a single batch was used to avoid lot-to-lot variations.
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