S8ap0

Manufactured by Leica camera

Sourced in Germany

The S8AP0 is a compact and versatile laboratory microscope designed for a wide range of applications. It features a binocular viewing head, a standard Abbe condenser, and a built-in LED illumination system. The microscope provides high-quality optics and a stable, durable construction to support precision imaging and analysis in a laboratory setting.

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Lab products found in correlation

45 protocols using s8ap0

Charcoalified Eurya Fruits and Seeds Analysis

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More than 1000 specimens of charcoalified fruits and seeds were collected from the fossil site. Among them, more than 80 seeds and seed fragments of Eurya were identified through observations under a binocular microscope (Leica, S8AP0). The fossil seeds were cleaned by an ultrasonic cleaner at 40 kHz (KO-50M) for 5–10 s. Air dried, they were then observed under a 3D Super Depth Digital Microscope (ZEISS Smartzoom 5) and images were taken. Five seed specimens were further studied under a scanning electron microscope (SEM, Zeiss EVO LS10) both morphologically and anatomically. For comparative analysis, extant seeds of Eurya obtained from herbarium specimens housed at the Herbarium of Kunming Institute of Botany (KUN) were also examined using the same procedure as the fossils. The descriptive terminology mainly follows Friis (1985) . All studied fossil specimens are numbered and kept at the KUN.

Zhu H., Huang Y.J., Su T, & Zhou Z.K. (2016). New fossil seeds of Eurya (Theaceae) from East Asia and their paleobiogeographic implications. Plant Diversity, 38(3), 125-132.

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Bamboo Fungus Morphological Characterization

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Bamboo culms with large, reddish to pale yellow ascostromata were collected from Yunnan, China and brought to the laboratory in 2017. Samples were examined following the methods described in Dai et al. (2017) (link). Micro-morphological characters were examined and photographed by differential interference contrast (DIC), using a Leica DM2500 compound microscope with a Leica DMC4500 camera. Fruiting bodies were observed by stereomicroscopy using a Leica S8AP0 and photographed by HDMI 200C. Measurements were made using Tarosoft (R) Image Frame Work software. Specimens have been deposited at the herbarium of Kunming Institute of Botany, Chinese Academy of Sciences (

KUN

) and Herbarium Mycologicum, Academiae Sinicae (HMAS) in Beijing. Facesoffungi (Jayasiri et al. 2015 (link)) and Index Fungorum (Index Fungorum 2019 ) numbers were provided for new taxa. Type material of H.bambusae was loaned and examined from the Royal Botanic Gardens, Kew.

Dai D.Q., Wijayawardene N.N., Tang L.Z., Liu C., Han L.H., Chu H.L., Wang H.B., Liao C.F., Yang E.F., Xu R.F., Li Y.M., Hyde K.D., Bhat D.J, & Cannon P.F. (2019). Rubroshiraia gen. nov., a second hypocrellin-producing genus in Shiraiaceae (Pleosporales). MycoKeys, 58, 1-26.

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Vascular Graft Patency Assessment via Ultrasound

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Sixty-one Sprague Dawley rats were randomly divided into two groups for transplantation of OM and ES vascular grafts. The transplantation procedure was the same as our previous study [11 (link)]. At the predetermined time point (3 days, 2 weeks, 4 weeks, and 12 weeks), the rats were anesthetized to analyze the patency of the grafts by high-resolution ultrasound (Vevo 2100 System, Visualsonics, Canada). After that, the rats were sacrificed by injection of overdose chloral hydrate, and the implanted grafts were collected. Each explanted graft was cut in half in the middle. One part was put into optimal cutting temperature (OCT, Tissue Tek) compound and snap-frozen by liquid nitrogen for frozen cross-sections. The other part was longitudinally cut into two pieces. After observed under a stereomicroscope (LEICA S8AP0, Germany), one piece was embedded in OCT snap-frozen by liquid nitrogen for longitudinal sections for CD31 staining. The other piece was fixed by 2.5% glutaraldehyde for the SEM examination, as noted in 2.4.

Wu P., Wang L., Li W., Zhang Y., Wu Y., Zhi D., Wang H., Wang L., Kong D, & Zhu M. (2020). Construction of vascular graft with circumferentially oriented microchannels for improving artery regeneration. Biomaterials, 242, 119922.

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Quantifying Root Morphology Using ImageJ

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ImageJ software (https://imagej.nih.gov/ij) was used to measure the root length, diameter, lateral root density, and area of cavities. For the calculation of lateral roots, all primary roots were pre-treated with a whole-mount clearing technique (Berleth and Jürgens, 1993 ). Briefly, roots were mounted in a mixture of chloralhydrate/glycerol/water (8:1:3) and cleared for 2 d at room temperature. Observations were carried out under a stereo microscope (Leica S8AP0, Germany). Both emerged lateral roots and lateral root primordial were counted as lateral roots. All experiments were performed at least three times.

Qu L., Wu C., Zhang F., Wu Y., Fang C., Jin C., Liu X, & Luo J. (2016). Rice putative methyltransferase gene OsTSD2 is required for root development involving pectin modification. Journal of Experimental Botany, 67(18), 5349-5362.

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Insect Ovary Imaging and Analysis

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Images of insects and ovaries were captured with a DFC320 digital camera attached to a LEICA S8AP0 stereomicroscope using the digital imaging system LAS (v. 3.8). Statistical analysis was performed using SPSS (v. 20) and Microsoft Excel. Means were compared using two-tailed Student's t-test at the significance levels set at *p < 0.05 and **p ≤ 0.01.

Zhang J.L., Yuan X.B., Chen S.J., Chen H.H., Xu N., Xue W.H., Fu S.J., Zhang C.X, & Xu H.J. (2018). The histone deacetylase NlHDAC1 regulates both female and male fertility in the brown planthopper, Nilaparvata lugens. Open Biology, 8(12), 180158.

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Daphnia magna Oviposition Observation

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We transferred Daphnia magna that underwent molting to a petri-dish with a small amount of M4 medium. Two different microscopes were used to record the oviposition process. A stereo-microscope (Leica S8AP0) was used to observe whole animals, while a light microscope (Olympus IX81) was used to focus on the egg canal. For recording, we used Las EZ 3.3.0 for the stereo-microscope and MetaMorph 7.6.5.0 for the light microscope.

Lee D., Nah J.S., Yoon J., Kim W, & Rhee K. (2019). Live observation of the oviposition process in Daphnia magna. PLoS ONE, 14(11), e0224388.

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GUS Reporter and Pectin Detection

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Histochemical analysis of the GUS reporter enzyme was performed essentially according to the method described by Jefferson et al. (1987) (link). Sample tissues were incubated in reaction buffer for 2 d and the GUS staining pattern was observed under a stereomicroscope (Leica S8AP0, Germany). For acidic (unesterified) pectin detection, root tips were acquired and embedded with 5% low-melting-point agarose. Then sections (50 μm thick) were obtained with a vibratome (Leica, RM2265) and stained by 0.02% Ruthenium Red for 30min, followed by rinsing and observation under a microscope.

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Microscopic Examination of Rice Grains

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Representative rice was arranged neatly in a dark room. The appearance of the white rice was photographed with a stereo microscope (Leica S8AP0, Germany).

Fan P., Xu J., Wang Z., Liu G., Zhang Z., Tian J., Wei H, & Zhang H. (2023). Phenotypic differences in the appearance of soft rice and its endosperm structural basis. Frontiers in Plant Science, 14, 1074148.

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Spider Specimen Imaging and Archiving

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Specimens were photographed with a Canon EOS 7D camera attached to an Olympus SZX16 stereomicroscope and Leica DFC295 camera connected to a stereo microscope Leica S8AP0. SEM figures were made with a SEM JEOL JSM-5200 scanning microscope at the Zoological Museum, University of Turku, Finland and with a Zeiss Ultra Plus SEM device at the Anadolu University, Eskişehir. Digital images were montaged using CombineZP image stacking software. The epigyne was cleared in a KOH/water solution until soft tissues were dissolved. Photographs were taken in dishes with cotton or paraffin on the bottom to hold the specimens in position. All measurements are given in mm. Materials studied here are deposited in the Zoological Museum of the Moscow State University (

ZMMU

), Zoological Institute of St-Petersburg (

ZISP

), Zoological Museum, University of Turku (

ZMUT

), and Anadolu University, Zoological Museum (

AUZM

Özkütük R.S., Marusik Y.M., Kunt K.B, & Elverici M. (2017). Taxonomic notes on two sibling species of Metellina from Asia (Araneae, Tetragnathidae). ZooKeys.

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Subcutaneous Implantation of Tissue-Engineered Constructs

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Templates with inner diameters of 2, 3, and 5 mm were implanted subcutaneously in the dorsum of rats (nPS and hPS), rabbits (hMPS), dogs (hMPS), and sheep (hMPS), respectively. A small incision was made, and a template of appropriate length was inserted into the subcutaneous pouch. The skin was closed intracutaneously. Thirty days after SI, templates and the surrounding tissue capsules were gently removed from the subcutaneous tissue. After harvest, the ends of the templates were blunted. Then, PBs, including nPB and hPB, were obtained by removing silicone tubes and silicone rods from the tissue capsule. Specialized shape templates were embedded into subcutaneous pouches of rabbits to form hMPB. Silicone rods (outer diameter 2 mm) were used to fabricate TB in rats. The morphological characterization of nPS, hPS, nPB, hPB, TB, and rAA was observed by stereomicroscopy (S8AP0, Leica). Body weight and temperature of all rats were recorded before and after SI.

Zhi D., Cheng Q., Midgley A.C., Zhang Q., Wei T., Li Y., Wang T., Ma T., Rafique M., Xia S., Cao Y., Li Y., Li J., Che Y., Zhu M., Wang K, & Kong D. (2022). Mechanically reinforced biotubes for arterial replacement and arteriovenous grafting inspired by architectural engineering. Science Advances, 8(11), eabl3888.

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