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Octrotide

Manufactured by Merck Group
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Octrotide is a synthetic peptide medication used in the treatment of various medical conditions. It functions as an analogue of the natural hormone somatostatin, which regulates the secretion of other hormones in the body. Octrotide is commonly utilized in the management of acromegaly, neuroendocrine tumors, and other related disorders.

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Lab products found in correlation

Somatostatin, by Merck Group (2 mentions) Vx 809, by Selleck Chemicals (2 mentions) Secretin, by Merck Group (2 mentions)

2 protocols using octrotide

1

Organoid Size Regulation by Secretagogues

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Images of CLC organoids were taken using 5X magnification before and following the addition of secretin (100nM, Sigma Aldrich), somatostatin (100nM, Sigma Aldrich), octrotide (100nM, Sigma Aldrich) or embryo transfer water serving as a negative control, at 0.5 - 2 minute intervals until organoid size stabilized. To explore the impact of octreotide on the effect of secretin, cells were pre-incubated for 30 minutes with octreotide. 100nM of secretin (Sigma Aldrich) was subsequently added to the medium and the experiment was carried out as described above. To assess the effect of VX809 on organoid size images were taken before and 6 hours following the addition of VX809 (30mM, Selleck) or embryo transfer water, serving as a negative control. 3 random diameters were measured for 8 random organoids pre and post treatment. Graph measurements represent percentage differences in mean organoid diameter. Error bars represent SD. Statistical significance was calculated using one-way ANOVA with Dunnett correction for multiple comparisons. The videos available as online supplementary data were made by taking images pre and post treatment at 2 minute intervals, until organoid size stabilized.
Sampaziotis F., de Brito M.C., Madrigal P., Bertero A., Saeb-Parsy K., Soares F.A., Schrumpf E., Melum E., Karlsen T.H., Bradley J.A., Gelson W.T., Davies S., Baker A., Kaser A., Alexander G.J., Hannan N.R, & Vallier L. (2015). Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nature biotechnology, 33(8), 845-852.
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2

Organoid Size Regulation by Secretagogues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images of CLC organoids were taken using 5X magnification before and following the addition of secretin (100nM, Sigma Aldrich), somatostatin (100nM, Sigma Aldrich), octrotide (100nM, Sigma Aldrich) or embryo transfer water serving as a negative control, at 0.5 - 2 minute intervals until organoid size stabilized. To explore the impact of octreotide on the effect of secretin, cells were pre-incubated for 30 minutes with octreotide. 100nM of secretin (Sigma Aldrich) was subsequently added to the medium and the experiment was carried out as described above. To assess the effect of VX809 on organoid size images were taken before and 6 hours following the addition of VX809 (30mM, Selleck) or embryo transfer water, serving as a negative control. 3 random diameters were measured for 8 random organoids pre and post treatment. Graph measurements represent percentage differences in mean organoid diameter. Error bars represent SD. Statistical significance was calculated using one-way ANOVA with Dunnett correction for multiple comparisons. The videos available as online supplementary data were made by taking images pre and post treatment at 2 minute intervals, until organoid size stabilized.
Sampaziotis F., de Brito M.C., Madrigal P., Bertero A., Saeb-Parsy K., Soares F.A., Schrumpf E., Melum E., Karlsen T.H., Bradley J.A., Gelson W.T., Davies S., Baker A., Kaser A., Alexander G.J., Hannan N.R, & Vallier L. (2015). Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nature biotechnology, 33(8), 845-852.
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