Hrp conjugated secondary antibodies goat anti rabbit and goat anti mouse

Tetrandrine was purchased from Shanghai Ronghe Medical, Inc. (Shanghai, China) and dissolved in DMSO for use. DCFH-DA was obtained from Invitrogen (Carlsbad, CA). 3-Methyladenine (3-MA) and N-acetyl-L-cysteine were purchased from Sigma (St. Louis, MO). Acridine orange (AO), GAPDH antibody and HRP-conjugated secondary antibodies (goat anti-rabbit and goat anti-mouse) were purchased from Beyotime (Nantong, China). The antibody against microtubule-associated protein 1 light chain 3 (LC3) was purchased from Sigma (St. Louis, MO). The p62 antibody was obtained from Cell Signaling Technologies (Beverly, MA), and cathepsin D (CTSD) was acquired from Proteintech Group, Inc. (Chicago, IL).

Tetrandrine was purchased from Shanghai Ronghe Medical, Inc. (Shanghai, China) and was stored at −80°C as a powder and dissolved in dimethyl sulfoxide before use. 3-Methyladenine and N-acetyl-L-cysteine were purchased from Sigma (St. Louis, MO). DCFH-DA was obtained from Invitrogen (Carlsbad, CA). DAPT was obtained from Selleck (Shanghai, China). Acridine orange, NBT, the GAPDH antibody and HRP-conjugated secondary antibodies (goat anti-rabbit and goat anti-mouse) were purchased from Beyotime (Nantong, China). The antibody against microtubule-associated protein 1 light chain 3 (LC3) was purchased from Sigma (St. Louis, MO). CD14-FITC and CD11b-PE were obtained from BD Biosciences. Other antibodies were obtained from the following sources: p27kip1, PARP, p62, HES1, Notch1 were from Cell Signaling Technologies (Beverly, MA); CD14, cathepsin D (CTSD), and p21 were acquired from Proteintech Group Inc. (Chicago, IL).