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Ghost dye a710 viability stain

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Ghost dye A710 viability stain is a fluorescent dye used to detect and quantify viable cells in a sample. It binds to DNA and emits fluorescence upon excitation, allowing for the identification of live cells.

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Lab products found in correlation

Cd3 percp clone sk7, by BioLegend (2 mentions) Cd4 apc clone okt4, by BioLegend (2 mentions) Cd8 bv570 clone rpa t8, by BioLegend (2 mentions) Cd14 fitc clone 63d3, by BioLegend (2 mentions) Cd19 bv510 clone sj25c1, by BD (2 mentions) Facsaria fusion instrument, by BD (2 mentions)

Close spelling variants of this product

Ghost viability dye (6 citations) Ghost Dye (26 citations) Viability dye (6 citations)

2 protocols using ghost dye a710 viability stain

1

Detailed PBMC Immunophenotyping Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
1M PBMCs from each donor were stained using standard procedure (30 min, 4 C) with the following surface antibody panel (CD3-PerCP clone SK7 (BioLegend), CD4-APC clone OKT4 (BioLegend), CD8-BV570 clone RPA-T8 (BioLegend), CD14-FITC clone 63D3 (BioLegend), CD19-BV510 clone SJ25C1 (BD), and Ghost dye A710 viability stain (Tonbo)) (Life Sciences Reporting Summary). Samples were then analyzed and sorted using a BD FACSAria Fusion instrument at the UCSF flow cytometry core. To calculate cell type proportions, the number of events in each of CD3+ CD4+ CD8− (CD4+ T cells), CD3+ CD4− CD8+ (CD8+ T cells), CD3− CD19+ (B cells), and CD3− CD14+ (monocytes) were divided by the sum of events in these gates (Supplementary Fig. 21).
Kang H.M., Subramaniam M., Targ S., Nguyen M., Maliskova L., McCarthy E., Wan E., Wong S., Byrnes L., Lanata C., Gate R., Mostafavi S., Marson A., Zaitlen N., Criswell L.A, & Ye C.J. (2017). Multiplexed droplet single-cell RNA-sequencing using natural genetic variation. Nature biotechnology, 36(1), 89-94.
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2

Detailed PBMC Immunophenotyping Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
1M PBMCs from each donor were stained using standard procedure (30 min, 4 C) with the following surface antibody panel (CD3-PerCP clone SK7 (BioLegend), CD4-APC clone OKT4 (BioLegend), CD8-BV570 clone RPA-T8 (BioLegend), CD14-FITC clone 63D3 (BioLegend), CD19-BV510 clone SJ25C1 (BD), and Ghost dye A710 viability stain (Tonbo)) (Life Sciences Reporting Summary). Samples were then analyzed and sorted using a BD FACSAria Fusion instrument at the UCSF flow cytometry core. To calculate cell type proportions, the number of events in each of CD3+ CD4+ CD8− (CD4+ T cells), CD3+ CD4− CD8+ (CD8+ T cells), CD3− CD19+ (B cells), and CD3− CD14+ (monocytes) were divided by the sum of events in these gates (Supplementary Fig. 21).
Kang H.M., Subramaniam M., Targ S., Nguyen M., Maliskova L., McCarthy E., Wan E., Wong S., Byrnes L., Lanata C., Gate R., Mostafavi S., Marson A., Zaitlen N., Criswell L.A, & Ye C.J. (2017). Multiplexed droplet single-cell RNA-sequencing using natural genetic variation. Nature biotechnology, 36(1), 89-94.
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