The largest database of trusted experimental protocols

Nanozoomer 2ht

Manufactured by Hamamatsu Photonics
Sourced in Japan

The Nanozoomer 2HT is a high-throughput digital slide scanner developed by Hamamatsu Photonics. It is designed to quickly capture high-resolution digital images of microscope slides. The Nanozoomer 2HT utilizes advanced optical and imaging technologies to provide detailed, high-quality scans of tissue samples or other specimens.

Automatically generated - may contain errors

11 protocols using nanozoomer 2ht

1

Quantifying Muscle Fiber Abnormalities

Check if the same lab product or an alternative is used in the 5 most similar protocols
Transversal or longitudinal cryosections (8 μm) of TA and diaphragm or 5 μm from paraffin-embedded liver or kidney were prepared, fixed and stained by Haematoxylin and Eosin (H&E) or succinate dehydrogenase (SDH). Sections were imaged with the Hamamatsu NanoZoomer 2HT slide-scanner. The percentage of TA muscle fibres with centralized or internalized nuclei was counted using the cell counter plugin in Fiji image analysis software. The fibre area was measured using the Fiji software.
+ Open protocol
+ Expand
2

Hindlimb Muscle Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The hindlimb muscles were dissected, weighed, snap frozen in liquid nitrogen, cooled in isopentane, and stored at −80°C until processing. Transverse sections of TA muscles (8 μm width) were cut for H&E and SDH stainings and scanned in a NanoZoomer 2HT (Hamamatsu, Iwata, Japan) and processed using ImageJ software (NIH, Bethesda, MD, USA).
+ Open protocol
+ Expand
3

Immunohistochemical Staining of Neuronal Marker

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rehydrated sections were subjected to antigen retrieval by microwaving 2 × 8 min at 650 W in citric acid buffer (0.01 M, pH 6) followed by 10 min cooling in citric acid buffer and 20 min washing under cold tap water. Washed slides were incubated 10 min in 3% hydrogen peroxide prior to 10 min washing in PBS supplemented with 0.25% Triton X100 (PBS‐T). Slides were incubated over‐night at 4°C with primary antibody against the pan neuronal marker HuC/HuD (A‐21271, Thermo Fisher Scientific, SE, RRID AB_221448, (Cheng et al., 2016 (link))) diluted 1:400 in PBS with 0.25% BSA. Sections were washed 3 × 10 min in PBS‐T followed by 1 h incubation with SignalStain boost‐detection HRP‐mouse (8125S, Cell Signaling, USA, RRID AB_10547893). Slides were washed 3 × 10 min in PBS‐T followed by development for 1 min with DAB reagent (sk‐4100, Vector, USA, RRID AB_2336382). Slides were washed 10 min under running tap water before being submerged 30 s in hematoxylin for counterstaining. Following counterstaining slides were washed 10 min under running tap water and dehydrated by stepwise washes in increasing grades of ethanol and xylene before being mounted with Pertex™ (Histolab, SE). Slides were scanned on a Nanozoomer 2HT (Hamamatsu, JP) in brightfield mode with 20x objective and analyzed using QuPath software (Bankhead et al., 2017 (link)).
+ Open protocol
+ Expand
4

Histological and Immunoblot Analysis of Mouse Skeletal Muscles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Muscles and other tissues were frozen in nitrogen-cooled isopentane and liquid nitrogen for histological and immunoblot assays, respectively. Longitudinal and transverse 8 µm cryosections of mouse skeletal muscles were prepared, fixed and stained with antibodies against DHPRα 1 (1:100), RYR1 (1:200), α-actinin (1:1000), DNM2-R2680 (1:200), MTM1-R2827 (1:200), pan-isoform BIN1-C99D (1:50) and BIN1-R2444 (1:100) antibodies. Nuclei were detected by co-staining with Hoechst (Sigma-Aldrich) for 10 min. Samples were viewed using a TCS SP5 laser scanning confocal microscope (Leica). Air-dried transverse sections were fixed and stained with H&E, SDH, NADH-TR or Sirius Red/Fast Green staining and image acquisition performed with a slide scanner NanoZoomer 2 HT equipped with the fluorescence module L11600-21 (Hamamatsu Photonics) or a DMRXA2 microscope (Leica). Cross-sectional area (CSA) was analyzed in H&E sections from TA skeletal muscle using FIJI image analysis software. CSA (μm2) was calculated (>500 fibers per mouse) from 4 to 7 mice per group. The percentage of TA muscle fibers with centralized or internalized nuclei was counted in >500 fibers from 4 to 6 mice using the cell counter plug-in in ImageJ image analysis software.
+ Open protocol
+ Expand
5

Paraffin-Embedded Tumour Sectioning

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumours were paraffin-embedded and serially sectioned. Transverse tumour sections were scanned at 40× magnification on a Nanozoomer 2HT (Hamamatsu) equipped for bright field and fluorescence acquisition (triple filter cube DAPI/FITC/TxRed) or images of each section were acquired using a fluorescence optical microscope equipped with magnification objective lens.
+ Open protocol
+ Expand
6

Quantitative Analysis of gpNMB Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
gpNMB was stained using goat anti-human gpNMB (R&D Systems) primary antibody (1:500) at 4°C overnight, then with an anti-goat-Dylight 488 (Novus) secondary antibody (1:2500) at ambient temperature for 1 hr. Slides were counterstained with DAPI mounting solution (Sigma). The high-resolution images of tissue slices were acquired using a digital whole slide scanner (Nanozoomer 2-HT, Hamamatsu. Bridgewater, NJ) using a 20×/0.0.75 lens (Olympus, Center Valley, PA). The images were then quantified using the image analysis module of a digital pathology software Visiomorph (VisioPharm, Broomfield, CO).
+ Open protocol
+ Expand
7

Analyzing Skeletal Muscle Fiber Characteristics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Air-dried transverse cryosections (8 μm) were fixed and stained with Hematoxylin and Eosin (HE) or SDH, and image acquisition performed with a slide scanner NanoZoomer 2 HT equipped with brightfield and the fluorescence module L11600-21 (Hamamatsu Photonics). Minimum feret's diameter was analyzed in wheat germ agglutinin (WGA) sections from TA mouse skeletal muscle, using a plugin developed in ImageJ – ‘MyoMage’. Minimum feret's diameter was calculated in >500 fibers per mouse. The percentage of TA muscle fibers with centralized or internalized nuclei was counted in >500 fibers using the cell counter plugin in ImageJ (http://rsb.info.nih.gov/ij/) or FIJI analysis software. Qualitative SDH staining analysis was performed. Fiber to fiber variation in intensity in SDH staining is normal and is indicative of the oxidative state of the fiber. SDH staining is normally relatively homogeneous within each individual fiber. Accumulation within the center or the periphery of a fiber of SDH staining indicates an abnormal distribution.
+ Open protocol
+ Expand
8

Paraffin-Embedded Tumour Sectioning

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumours were paraffin-embedded and serially sectioned. Transverse tumour sections were scanned at 40× magnification on a Nanozoomer 2HT (Hamamatsu) equipped for bright field and fluorescence acquisition (triple filter cube DAPI/FITC/TxRed) or images of each section were acquired using a fluorescence optical microscope equipped with magnification objective lens.
+ Open protocol
+ Expand
9

Quantitative Histological Analysis of Mouse Skeletal Muscle

Check if the same lab product or an alternative is used in the 5 most similar protocols
Air-dried transverse cryosections (8 μm) were fixed and stained with hematoxylin and eosin (H&E) or succinate dehydrogenase (SDH), and image acquisition performed with a slide scanner NanoZoomer 2 HT equipped with the fluorescence module L11600-21 (Hamamatsu Photonics, Japan). Cross-sectional area (CSA) was analyzed in WGA sections from TA mouse skeletal muscle, using a plugin developed in ImageJ. CSA (μm2) was calculated in >500 fibers per mouse. The percentage of TA muscle fibers with centralized or internalized nuclei was counted in >500 fibers using the cell counter plugin in ImageJ (Rasband, W.S., ImageJ, National Institutes of Health, Bethesda, MD, USA, http://rsb.info.nih.gov/ij/, 1997–2009) or FIJI analysis software. Qualitative SDH staining analysis was performed. Fiber to fiber variation in intensity in SDH staining is normal and is indicative of the oxidative state of the fiber. SDH staining is normally relatively homogeneous within each individual fiber. Accumulation within the center or the periphery of a fiber of SDH staining indicates an abnormal distribution.
+ Open protocol
+ Expand
10

Epifluorescence and Confocal Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were observed with an epifluorescence microscope (DM4000, Leica) using 10x (numerical aperture (NA): 0.25), 20x (NA:0.7), 40x (NA:0.75) or 63x (NA:1.32) objectives, zoom 1 or 0.55 with a CCD camera CoolSnap or with a confocal microscope (SP2RS, Leica) using 40x (NA:1.25) and 63x (NA:1.4) objectives zoom 1 or 4 with the LCS (Leica) software for image acquisition. Image acquisition was also performed with the slide scanner NanoZoomer 2 HT equipped with the fluorescence module L11600-21 (Hamamatsu Photonics, Japan).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!