Biacore cm5 sensor chips

Manufactured by Cytiva

The Biacore CM5 sensor chips are used in Biacore systems for label-free interaction analysis. They provide a carboxymethylated dextran surface for immobilization of ligands, enabling the study of biomolecular interactions.

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Lab products found in correlation

Ethanolamine, by GE Healthcare (1 mentions) Flag m2 antibody, by Merck Group (1 mentions) P 20 surfactant, by GE Healthcare (1 mentions)

Close spelling variants of this product

CM5 sensor chip (145 citations) CM5 chip (57 citations) Biacore CM5 (4 citations) CM5 Biacore chip (3 citations) CM5 sensor (3 citations)

2 protocols using biacore cm5 sensor chips

Binding Kinetics of DPI-4452

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Recombinant CA proteins (Sino Biologic) were immobilized on Biacore CM5 sensor chips or biotin CAPture chips (Biacore). DPI-4452 binding was measured using the Biacore Liquid System (Biacore), and binding kinetic parameters were calculated.

Massière F., Wiedemann N., Borrego I., Hoehne A., Osterkamp F., Paschke M., Zboralski D., Schumann A., Bredenbeck A., Brichory F, & Attinger A. (2024). Preclinical Characterization of DPI-4452: A 68Ga/177Lu Theranostic Ligand for Carbonic Anhydrase IX. Journal of Nuclear Medicine, 65(5), 761-767.

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Surface Plasmon Resonance Assay

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The surface plasmon resonance assays were conducted on a Biacore T200 instrument using Biacore CM5 sensor chips (Biacore). Ethanolamine, N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) and P-20 surfactant were all obtained from GE Lifesciences. M2 Flag antibody was obtained from Sigma. Reference proteins were obtained from Origene.

Pop M.S., Stransky N., Garvie C.W., Theurillat J.P., Lewis T.A., Zhong C., Culyba E.K., Lin F., Daniels D.S., Pagliarini R., Ronco L., Koehler A.N, & Garraway L.A. (2014). A small molecule that binds and inhibits the ETV1 transcription factor oncoprotein. Molecular cancer therapeutics, 13(6), 1492-1502.

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