Mammalian expression plasmid encoding human TMEM263 with a C-terminal epitope tag (Myc-DDK) was obtained from Origene (RC203933). Control pCDNA3.1 empty plasmid was obtained from Invitrogen. Rabbit polyclonal anti-human C12orf23 (TMEM263) was from Origene (TA333490). Mouse monoclonal JAK2 (C-10), STAT5 (A-9), growth hormone receptor (GHR) (B-12) were obtained from Santa Cruz (sc-390539, sc-74442, and sc-137184, respectively). Rabbit monoclonal antibody against phospho-JAK2 (Tyr1008)(D4A8) was obtained from Cell Signaling Technology (CST # 8082). Rabbit polyclonal antibody against phospho-STAT5 (Y694) was obtained from Cell Signaling Technology (CST#9351). Mouse monoclonal anti-FLAG M2 antibody (F1804) and mouse monoclonal anti-β-actin antibody (A1978) were obtained from Sigma. HRP-conjugated goat anti-rabbit (CST#7074) and goal anti-mouse (CST#7076) antibodies were from Cell Signaling Technology.
Control pcdna3.1 empty plasmid
Manufactured by Thermo Fisher Scientific
The control pcDNA3.1 empty plasmid is a circular DNA molecule that can be used in molecular biology experiments as a control vector. It does not contain any gene of interest, serving as a baseline for comparison in experiments involving gene expression or other plasmid-based manipulations.
Automatically generated - may contain errors
Lab products found in correlation
Hrp conjugated goat anti rabbit, by Cell Signaling Technology (2 mentions)
Mouse monoclonal anti β actin antibody a1978, by Merck Group (2 mentions)
Tyr1008 d4a8, by Cell Signaling Technology (2 mentions)
Rabbit polyclonal anti human c12orf23 tmem263, by OriGene (2 mentions)
Mouse monoclonal anti flag m2 antibody f1804, by Merck Group (2 mentions)
Sc 74442, by Santa Cruz Biotechnology (1 mentions)
2 protocols using control pcdna3.1 empty plasmid
1
Expression and Characterization of TMEM263
2
Characterization of TMEM263 protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mammalian expression plasmid encoding human TMEM263 with a C-terminal epitope tag (Myc-DDK) was obtained from Origene (RC203933). Control pCDNA3.1 empty plasmid was obtained from Invitrogen. Rabbit polyclonal anti-human C12orf23 (TMEM263) was from Origene (TA333490). Mouse monoclonal JAK2 (C-10), STAT5 (A-9), growth hormone receptor (GHR)(B-12) were obtained from Santa Cruz (sc-390539, and sc-74442, and sc-137184, respectively). Rabbit monoclonal antibody against phospho-JAK2 (Tyr1008)(D4A8) were obtained from Cell Signaling Technology (CST # 8082). Rabbit polyclonal antibody against phospho-STAT5 (Y694) was obtained from Cell Signaling Technology (CST#9351). Mouse monoclonal anti-FLAG M2 antibody (F1804) and mouse monoclonal anti-β-actin antibody (A1978) were obtained from Sigma. HRP-conjugated goat anti-rabbit (CST#7074) and goal anti-mouse (CST#7076) antibodies were from Cell Signaling Technology.
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