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T7 mscript standard mrna production system kit

Manufactured by CellScript
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The T7 mScript Standard mRNA Production System Kit is a comprehensive solution for the in vitro transcription of mRNA. The kit includes the necessary components to efficiently synthesize capped and polyadenylated mRNA from a DNA template using T7 RNA polymerase.

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Lab products found in correlation

Geneart, by Thermo Fisher Scientific (2 mentions) Megaclear transcription clean up kit, by Thermo Fisher Scientific (2 mentions) Rna tapestation, by Agilent Technologies (1 mentions)

2 protocols using t7 mscript standard mrna production system kit

1

Codon-Optimized Mammalian Expression

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Human codon-optimized constructs were synthesized (GeneArt, Thermo Fisher Scientific) and cloned into the mammalian expression vector pCI (Promega). Cap 1 mRNA was synthesized with the T7 mScript Standard mRNA Production System Kit (CELLSCRIPT) and purified by the MEGAclear Transcription Clean-Up Kit (Thermo Fisher Scientific) per manufacturers’ instructions. The mRNA purity, integrity, and transcript size were confirmed by RNA TapeStation analysis (Agilent) prior to electroporation.
Hwang M.S., Mog B.J., Douglass J., Pearlman A.H., Hsiue E.H., Paul S., DiNapoli S.R., Konig M.F., Pardoll D.M., Gabelli S.B., Bettegowda C., Papadopoulos N., Vogelstein B., Zhou S, & Kinzler K.W. (2021). Targeting loss of heterozygosity for cancer-specific immunotherapy. Proceedings of the National Academy of Sciences of the United States of America, 118(12), e2022410118.
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2

Codon-optimized Construct Synthesis and mRNA Production

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Human codon-optimized constructs were synthesized (GeneArt, Thermo Fisher Scientific, Waltham, MA) and cloned into the mammalian expression vector pCI (Promega, Madison, WI). Per manufacturers’ instructions, the T7 mScript Standard mRNA Production System Kit (CELLSCRIPT, Madison, WI) was used to synthesize Cap 1 mRNA, followed by purification using the MEGAclear Transcription Clean-Up Kit (Thermo Fisher Scientific, Waltham, MA). Prior to electroporation, RNA TapeStation (Agilent, Santa Clara, CA) analysis was performed to determine mRNA purity, integrity, and transcript size.
Hwang M.S., Miller M.S., Thirawatananond P., Douglass J., Wright K.M., Hsiue E.H., Mog B.J., Aytenfisu T.Y., Murphy M.B., Aitana Azurmendi P., Skora A.D., Pearlman A.H., Paul S., DiNapoli S.R., Konig M.F., Bettegowda C., Pardoll D.M., Papadopoulos N., Kinzler K.W., Vogelstein B., Zhou S, & Gabelli S.B. (2021). Structural engineering of chimeric antigen receptors targeting HLA-restricted neoantigens. Nature Communications, 12, 5271.
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