Twenty-seven patients diagnosed with XDR-GNB BSI were retrospective reviewed in this study between January 1, 2013, and December 31, 2017 in Department of Hematology, the First Affiliated Hospital of USTC (University of Science and Technology of China). XDR-GNB was defined as resistant to almost all classes of antibiotics except one or two classes of antibiotics (mainly polymyxin and tigecycline).3 (link)–5 (link) The BacT/ALERT 3D automated blood culture system (BioMérieux, Lyon, France) was used to process all blood samples. All strains were identified by Gram stain and the VITEK 2-compact automated system (BioMerieux). Antimicrobial susceptibility testing was also carried out using the VITEK 2-compact system. The susceptibility of the organism tested was determined according to the Clinical and Laboratory Standards Institute recommendations 2013 edition. Quality control strains were included in each batch of antimicrobial testing to ensure the accuracy of the results.
Bact alert 3d automated blood culture system
Manufactured by bioMérieux
Sourced in France, United States
The BacT/Alert 3D is an automated blood culture system manufactured by bioMérieux. The system is designed to detect the presence of microorganisms in blood samples.
Automatically generated - may contain errors
Lab products found in correlation
Vitek 2 compact automated system, by bioMérieux (1 mentions)
Bactec automated culture system, by BD (1 mentions)
Bactec fx, by BD (1 mentions)
Elecsys brahms pct kit, by Roche (1 mentions)
Cobas 8000 modular analyzer, by Roche (1 mentions)
Xn 550, by Sysmex (1 mentions)
376 electronic baby scale, by Seca (1 mentions)
6 protocols using bact alert 3d automated blood culture system
1
Retrospective Review of XDR-GNB BSI Patients
2
Automated Blood Culture Protocol
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Blood samples obtained from auricular artery were inoculated into BacT/Alert PF bottles (bioMérieux, Inc., Durham, NC, USA). The bottles were then processed in a BacT/Alert 3D automated blood culture system (bioMérieux, Inc., Durham, NC, USA). The bottles signaled as positive were removed from the instrument, and the samples were inoculated into blood agar plates and MacConkey plates, cultured with 5% CO2 at 37 °C for 24 h. Bacterial identifications were performed by the VITEK-2 COMPACT fully automated microbiological system (bioMérieux, Inc., Durham, NC, USA).
3
Automated Blood Culture Protocol
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According to the Chinese health regulatory requirements for bacterial culture, all blood samples were collected, sorted, and cultured. The Bact/Alert3D automated blood culture system (BioMérieux, France) and VITEK2-compact mass spectrometer were employed for automated blood culture, bacterial identification, and drug sensitivity studies, supporting both aerobic and anaerobic culture bottles.
The antimicrobial susceptibility analysis was conducted following the standards set by the American Institute of Clinical and Laboratory Standards (CLSI), and the interpretation of the results was based on CLSI standards.912 (no links found)
The antimicrobial susceptibility analysis was conducted following the standards set by the American Institute of Clinical and Laboratory Standards (CLSI), and the interpretation of the results was based on CLSI standards.9
4
Enteric Fever Surveillance Protocol
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Whole blood was collected before treatment and incubated for up to 5 days using the BACTEC automated culture system (Becton Dickinson, Franklin Lakes, NJ, USA), or the BacTAlert 3D automated blood culture system (BioMérieux, Marcy-l’Étoile, France). Positive samples were subcultured onto MacConkey agar, chocolate agar, or sheep blood agar (or a combination of these), and species were confirmed using biochemical testing and O and H antisera (MAST ASSURE, Mast Group, Liverpool, UK), if available.19 (link) Isolates obtained from laboratory network sites were confirmed at the study hospital laboratories using the same methods. All hospital laboratories were enrolled in WHO or College of American Pathologists external quality assurance programmes.
To estimate the proportion of individuals with a febrile illness in the catchment area compatible with enteric fever who sought care at the study facilities, interviewers administered a standard questionnaire to all households within randomly selected clusters of the defined catchment areas. The design and implementation of the health-care utilisation survey, including sample size calculations, are described elsewhere.20 (link), 21 (link)
We defined disease severity by hospitalisation. Hospitalisation at the enrolment visit was documented in the medical charts, and hospitalisation during the 6-week follow-up period was patient-reported.
To estimate the proportion of individuals with a febrile illness in the catchment area compatible with enteric fever who sought care at the study facilities, interviewers administered a standard questionnaire to all households within randomly selected clusters of the defined catchment areas. The design and implementation of the health-care utilisation survey, including sample size calculations, are described elsewhere.20 (link), 21 (link)
We defined disease severity by hospitalisation. Hospitalisation at the enrolment visit was documented in the medical charts, and hospitalisation during the 6-week follow-up period was patient-reported.
5
Diagnostic Surveillance for Pneumonia
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Based on clinical and laboratory findings, microbiological surveillance cultures such as blood, urine, sputum, and abdominal fluid were performed. Chest X-ray was obtained in patients who were suspicious to pneumonia.
Blood culture was obtained by standard procedure. The automats used were the BacT/ALERT 3D-automated blood culture system (bioMérieux, Durham, NC, USA) and the BACTEC FX (BDDiagnostic Systems, Sparks, MD, USA) (FX) for rapid microbial detection.
Results were interpreted according to the clinical and laboratory standards institute (CLSI) guideline.15 An expert infectious disease specialist exploited clinical guidelines of the centers for disease control (CDC) for diagnosis of PTIs.16 (link)
Blood culture was obtained by standard procedure. The automats used were the BacT/ALERT 3D-automated blood culture system (bioMérieux, Durham, NC, USA) and the BACTEC FX (BDDiagnostic Systems, Sparks, MD, USA) (FX) for rapid microbial detection.
Results were interpreted according to the clinical and laboratory standards institute (CLSI) guideline.15 An expert infectious disease specialist exploited clinical guidelines of the centers for disease control (CDC) for diagnosis of PTIs.16 (link)
6
Biomarkers and Outcomes in Sepsis
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The following data were extracted: demographic characteristics (age, gender), anthropometric measurements (body weight, height), evolution—including length of Intensive Care Unit (ICU) stay, mechanical ventilation and survival rate, primary diagnosis (infection, sepsis, septic shock) according to the definition by Goldstein et al. [30 (link)], and comorbidities. Body weight and height were measured (unclothed) with an infant scale using an attached infantometer (Seca 376 electronic baby scale; Seca Ltd., Hamburg, Germany). Laboratory analysis performed at the time of hospital admission or on the day of drawing blood culture included: CBC using an automated hematology analyzer (Sysmex XN-550, Sysmex Corporation, Kobe, Japan), CRP using a routine automated analyzer (Hitachi 747, Hitachi, Tokyo, Japan), and PCT levels using an electrochemiluminescence assay (Elecsys® BRAHMS PCT kit, Roche Diagnostic, Rotkreuz, Switzerland) on a Cobas® 8000 modular analyzer (Roche Diagnostic, Rotkreuz, Switzerland). Blood cultures drawn during fever were incubated in the BacT/Alert 3D automated blood culture system (BioMerieux, Craponne, France). Based on retrospectively available data, the following CBC-derived indices were calculated: NLR (neutrophil count/lymphocyte count) [31 (link)], SII (platelet count × NLR) [32 (link)], and SIRI (neutrophil count × monocyte/lymphocyte count) [33 (link)].
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