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Glass pipette

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Glass pipettes are laboratory instruments used to accurately measure and transfer small volumes of liquids. They are typically made of borosilicate glass and come in a range of sizes and capacities to suit various laboratory needs.

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Lab products found in correlation

C57 bl6j, by Narishige (2 mentions) Mo 10 manipulator, by Narishige (2 mentions) Aav1 cag mruby2 flag49, by Thermo Fisher Scientific (2 mentions) Wga alexa fluor 555, by Thermo Fisher Scientific (2 mentions) Paav hsyn egfp, by Addgene (1 mentions) Kwik sil, by World Precision Instruments (1 mentions) Nanojector, by Drummond (1 mentions) Mineral oil, by Thermo Fisher Scientific (1 mentions) P 2000 puller, by Sutter Instruments (1 mentions) Pclamp 10 analysis software, by Molecular Devices (1 mentions) Axopatch 200b amplifier, by Molecular Devices (1 mentions) Pp 83, by Narishige (1 mentions) Axopatch 1d amplifier, by Molecular Devices (1 mentions)

Spelling variants of this product

Pulled glass pipette (8 citations) Graduated glass pipettes (3 citations) Glass pipette and nanoject system (3 citations) Pre-calibrated glass microcapillary pipettes (2 citations) Pulled-beveled glass micro-pipettes (2 citations) Fine glass capillary pipettes (2 citations) Glass injection pipette (2 citations)

14 protocols using glass pipette

1

Viral Tracing in Gerbil Cortex

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Gerbils were first anesthetized with isoflurane/O2 and secured on a stereotaxic device (Kopf). The surgical scalp area was then shaved and cleaned with iodine and an incision was made to expose parietal, occipital, and frontal bones. For viral injections into parietal cortex, a craniotomy was made between 3.6–3.9 mm rostral and 2.5 mm lateral to lambda [46 ]. For viral injections into AC, the temporalis muscle was retracted and a craniotomy was made between 3.2–3.9 mm rostral to lambda and 1–1.5 mm ventral from the temporal ridge. A durotomy was performed over the region of interest and a glass pipette (Drummond) containing an anterograde (pAAV-hSyn-EGFP, Addgene plasmid 50465; pENN-AAV-hSyn-TurboRFP-WRPE-rBG, Addgene plasmid 105552) or retrograde tracer (pAAVrg-hSyn-EGFP, Addgene plasmid 50465) was attached to a microinjector (Nanoject). Two injections (200 nL) were made within each region of interest, first at 0.3 mm and then at 0.8 mm below the pial surface at an injection rate of 2 nL/sec. Following the injections, the exposed cortical surface was covered with a silicon elastomer (Kwik-Sil, World Precision Instruments) and the surgical incision was closed with sutures.
Yao J.D., Gimoto J., Constantinople C.M, & Sanes D.H. (2020). Parietal cortex is required for the integration of acoustic evidence. Current biology : CB, 30(17), 3293-3303.e4.
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2

Multifunctional Probes for In Vitro Testing

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Multifunctional probes composed of pulled glass pipettes and NEC devices were tested in vitro before implantation. The glass pipette (Drummond Scientific) coated with an NEC device as previously described was connected through a needle (gauge 30, BD) to a syringe controlled by an injection system (PHD 2000, Havard Apparatus) through ethylene-vinyl acetate tubing (0.5 mm inner diameter, McMaster-Carr). The injection rates were calibrated by injecting water and measuring the time and the output weight at the target rates of 1, 10, 20 and 50 nl/s. Before implantation, the needle was filled with 10 μL sterile PBS and sealed by a PDMS cap. Mice were allowed to recover for a week after surgical implantation of the probes. For drug infusion tests, the pre-filled PBS was exchanged by the same volume of 0.1 mM α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), followed by the injection of the same drug at 5 – 50 nl/s for 3 mins. Awake electrical recording were performed simultaneously to monitor the suppression and recovery of neural activities. The infusion was repeated after the neural activities recovered to the base line for multiple cycles.
Zhao Z., Luan L., Wei X., Zhu H., Li X., Lin S., Siegel J.J., Chitwood R.A, & Xie C. (2017). Nanoelectronic coating enabled versatile multi-functional neural probes. Nano letters, 17(8), 4588-4595.
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3

Stereotaxic Viral Injection and Optogenetic Implant

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Adult male mice were anesthetized by pentobarbital (80 mg/kg) and fixed with a stereotaxic apparatus (RWD Instruments). Five hundred nanoliters of virus was injected into the right SNC (AP = −3.16 mm, ML = −1.13 mm, DV = −4.4 mm) with an automated microsyringe pump (Drummond) fixed with a glass pipette (Drummond) pulled by a micropipette puller (Shutter Instruments, USA). After injection, the glass pipette was kept at a standstill for 5 min and then retracted. An optical fiber (core = 200 µm, numerical aperture [NA] = 0.37, length = 5 mm; RWD Instruments) was implanted into the same site of virus injection with a stereotactic cannula holder (RWD Instruments) and then fixed with dental cement (Yamahachi).
Li S., Wang D., Liu D., Meng X., Wang Z., Guo X., Liu Q., Liu P., Li S., Wang S., Yang R., Xu Y., Wang L, & Kang J. (2024). Neurotransmitter accumulation and Parkinson's disease‐like phenotype caused by anion channelrhodopsin opto‐controlled astrocytic mitochondrial depolarization in substantia nigra pars compacta. MedComm, 5(6), e568.
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4

Optogenetic Manipulation of mPFC Neurons

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Adult male PV-Cre+ rats (270 -320 g) were anesthetized with isoflurane. Viral vectors [Credependent pAAV-Flex-tdTomato-AAV9; AAV-hSyn-DIO-HM3Dq-mCherry; AVV-hSyn-DIO-HM4Di-mCherry (1.5 µl -3 µl)] were injected bilaterally into the mPFC using a stereotaxic apparatus. Injections were performed using a nanojector (Drummond Scientific Company, Broomall, PA, USA ) and a glass pipette (1 µm tip diameter) using the following coordinates for prelimbic mPFC: PL-PFC [anterior-posterior (AP): +3.0-3.2 mm; medial-lateral (ML): +/-0.7 mm; dorsal-ventral (DV): -3.8 mm, Paxinos and Watson 1982] . Rats were single housed for 7 days following surgery and then moved to pair housing for METH or SAL administration.
Armenta-Reséndiz M., Assali A., Ea T., Cowan C.W., & Lavı́n A. (2022). Chronic methamphetamine administration produces cognitive deficits through augmentation of GABAergic synaptic transmission in the prefrontal cortex.
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5

Volumetric Injection of Viral Vectors

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All procedures were performed according to the guidelines set by the Janelia Farm Research Campus Institutional Animal Care and Use Committee and Institutional Biosafety Committee. Animals (adult C57/BL6J, Charles River; either sex) mice were anesthetized under isoflurane and AAV virus encoding smFPs, serotype 2/1 was injected with a custom-made volumetric injection system (based on a Narishige MO-10 manipulator). Glass pipettes (Drummond) were pulled and beveled to a sharp tip (30 µm outer diameter), back-filled with mineral oil and front-loaded with viral suspension immediately before injection.
Viswanathan S., Williams M.E., Bloss E.B., Stasevich T.J., Speer C.M., Nern A., Pfeiffer B.D., Hooks B.M., Li W.P., English B.P., Tian T., Henry G.L., Macklin J.J., Patel R., Gerfen C.R., Zhuang X., Wang Y., Rubin G.M, & Looger L.L. (2015). High-performance probes for light and electron microscopy. Nature methods, 12(6), 568-576.
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6

Virus Injections for Neuronal Labeling in Mouse vS1

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Virus injection is conducted before the circular craniotomy was made. To label the pyramid neurons of layer 5 in vS1 cortex, virus containing jRGECO1a (AAV2/1.Syn.Flex.NES-jRGECO1a.WPRE.SV40, Penn Vector Core, genomic titer, 4.48×1013, dilution factor 6:1) or mRuby2 [32 ] (AAV2/1.hSyn.DIO.mRuby2, UCSD, genomic titer, 8.8×1012) was injected (50 nL, 10 nL per minute) at 45° angle into the target coordinates of the Rbp4-cre mice cortex: 1.5 mm posterior to Bregma, 3.4 mm lateral from midline and 0.7 mm depth. To label the thalamocortical projections in vS1 cortex, virus expressing SF-Venus-iGluSnFR ( AAV2/1.hSyn.Flex.SF-Venus-iGluSnFR.A184S, Janelia Research Campus, genomic titer, 1×1013) were mixed with AAV-Cre (AAV2/1.hSyn.Cre.WPRE.hGH, Addgene, genomic titer, 1×1013, dilution factor 1:100) at the ratio 1:1 and injected (50 nL, 10 nL per minute) perpendicular into the barreloids of ventral posteromedial nucleus in wide-type mice: 1.7 mm posterior to Bregma, 1.7 mm lateral from midline and 3.0 mm depth. For all injections glass pipettes (Drummond) were pulled and beveled to a sharp tip (30 μm outer diameter) and a syringe pump (Kd Science, Legato 185) was used to control the infusion.
Liu R., Li Z., Marvin J.S, & Kleinfeld D. (2019). Direct wavefront sensing enables functional imaging of infragranular axons and spines. Nature methods, 16(7), 615-618.
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7

Volumetric Injection of Viral Vectors

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All procedures were performed according to the guidelines set by the Janelia Farm Research Campus Institutional Animal Care and Use Committee and Institutional Biosafety Committee. Animals (adult C57/BL6J, Charles River; either sex) mice were anesthetized under isoflurane and AAV virus encoding smFPs, serotype 2/1 was injected with a custom-made volumetric injection system (based on a Narishige MO-10 manipulator). Glass pipettes (Drummond) were pulled and beveled to a sharp tip (30 µm outer diameter), back-filled with mineral oil and front-loaded with viral suspension immediately before injection.
Viswanathan S., Williams M.E., Bloss E.B., Stasevich T.J., Speer C.M., Nern A., Pfeiffer B.D., Hooks B.M., Li W.P., English B.P., Tian T., Henry G.L., Macklin J.J., Patel R., Gerfen C.R., Zhuang X., Wang Y., Rubin G.M, & Looger L.L. (2015). High-performance probes for light and electron microscopy. Nature methods, 12(6), 568-576.
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8

Targeted neural circuit manipulation

Cited 1 time
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ALM (AP 2.5 mm; ML 1.5mm; diameter 1.5 mm) is the cortical area that produced behavioral effects with photoinhibition during the delay epoch 3 (link),24 (link). For the thalamic reticular nucleus the coordinate was AP -0.7, ML 1.6, DV 3.7 - 3.3 mm, as retrograde labeling from thalALM showed labeling in this sector of the thalamic reticular nucleus (Extended Data Fig. 9). Virus and tracer were injected through the thinned skull using a volumetric injection system (modified from Mo-10 Narishige) 48 (link). Glass pipettes (Drummond) were pulled and beveled to a sharp tip (outer diameter ∼ 20 - 30 μm), back-filled with mineral oil and front-loaded with viral suspension immediately before injection. The injection rate was 15 nl/min. See Supplementary Table 2 for description of viruses and injection coordinates used for each experiment. We used the following viruses and tracers: AAV2/1-CAG-EGFP (Penn vector Core, University of Pennsylvania), AAV2/10 CAG-flex-ChR2(H134R)-tdTomato (Penn vector Core, University of Pennsylvania), AAV1-CAG-mRuby2-FLAG49 , WGA-Alexa Fluor® 555 (Thermo Fisher Scientific)50 (link) (WGA555), and Red RetroBeads (Lumafluor).
Guo Z.V., Inagaki H.K., Daie K., Druckmann S., Gerfen C.R, & Svoboda K. (2017). Maintenance of persistent activity in a frontal thalamocortical loop. Nature, 545(7653), 181-186.
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9

Targeted neural circuit manipulation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
ALM (AP 2.5 mm; ML 1.5mm; diameter 1.5 mm) is the cortical area that produced behavioral effects with photoinhibition during the delay epoch 3 (link),24 (link). For the thalamic reticular nucleus the coordinate was AP -0.7, ML 1.6, DV 3.7 - 3.3 mm, as retrograde labeling from thalALM showed labeling in this sector of the thalamic reticular nucleus (Extended Data Fig. 9). Virus and tracer were injected through the thinned skull using a volumetric injection system (modified from Mo-10 Narishige) 48 (link). Glass pipettes (Drummond) were pulled and beveled to a sharp tip (outer diameter ∼ 20 - 30 μm), back-filled with mineral oil and front-loaded with viral suspension immediately before injection. The injection rate was 15 nl/min. See Supplementary Table 2 for description of viruses and injection coordinates used for each experiment. We used the following viruses and tracers: AAV2/1-CAG-EGFP (Penn vector Core, University of Pennsylvania), AAV2/10 CAG-flex-ChR2(H134R)-tdTomato (Penn vector Core, University of Pennsylvania), AAV1-CAG-mRuby2-FLAG49 , WGA-Alexa Fluor® 555 (Thermo Fisher Scientific)50 (link) (WGA555), and Red RetroBeads (Lumafluor).
Guo Z.V., Inagaki H.K., Daie K., Druckmann S., Gerfen C.R, & Svoboda K. (2017). Maintenance of persistent activity in a frontal thalamocortical loop. Nature, 545(7653), 181-186.
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10

Stereotaxic Injection of Pertussis Toxin in Mice

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For stereotaxic surgeries, mice were anesthetized with isofluorane (2%–5%) and secured in a stereotaxic apparatus (Kopf). Glass pipettes (Drummond Scientific) were formed using a P-2000 puller (Sutter Instrument) and were characterized by a long taper and 10–20 μm diameter tips. Pipettes were back-filled with mineral oil (Fisher Scientific) before being loaded with pertussis toxin (Sigma P7208) and positioned over the lateral ventricle (coordinates relative to bregma, in mm: 0.25 lateral, 0.3 anterior, −3 ventral). A small drill hole was made in the skull to allow for pipette insertion. 1–2 μL of 0.1 g/L pertussis toxin were injected unilaterally into the ventricle. Experiments were performed 24–72 hours following injection. Throughout the surgery, body temperature, breathing and heart rate were monitored. Saline was administered subcutaneously (s.c) to maintain hydration and the animal was monitored post-operationally for signs of distress and discomfort. Buprenorphine (0.1 mg/kg s.c.) was given for analgesia. No major adverse effects of the surgery or pertussis toxin injection were observed.
Chamberland S., Nebet E.R., Valero M., Hanani M., Egger R., Larsen S.B., Eyring K.W., Buzsáki G, & Tsien R.W. (2023). Brief synaptic inhibition persistently interrupts firing of fast-spiking interneurons. Neuron, 111(8), 1264-1281.e5.
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