Imr 5 75

The human NB-1, IMR-5/75 and SH-SY5Y neuroblastoma cell lines were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Braunschweig, Germany) and cultivated in Roswell Park Memorial Institute (RPMI) 1640 media (Gibco Thermo Fischer Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (MilliporeSigma, Burlington, MA, USA) and 500 µg/mL geneticin (G418, MilliporeSigma, Burlington, MA, USA). The human SK-N-BE(2) neuroblastoma cell line was purchased from the American Type Culture Collection (Manassas, VA, USA), and cultivated in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fischer Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (MilliporeSigma, Burlington, MA, USA) and 500 µg/mL G418 (MilliporeSigma, Burlington, MA, USA). Cell line identity was confirmed using short tandem repeat DNA genotyping (Eurofins Scientific SE, Luxemburg, Luxemburg), and cultures were routinely tested for mycoplasma contamination using the PlasmoTest Kit (Thermo Fischer Scientific, Waltham, MA USA). All cells were cultured in a humidified atmosphere containing 5% CO₂ at 37 °C.