Pancreases or isolated islets from Wistar rats, GK rats and human subjects were fixed in 4% formalin, paraffin embedded and cut into 5‐μm sections. For immunofluorescence, sections were dewaxed and rehydrated as described above. All samples were subjected to antigen retrieval by heating in 0.01 M sodium citrate solution before immunolabelling. Sections were incubated overnight using antibodies targeting PYY (ab22663), insulin (in house), glucagon (G‐2654, Sigma, Gillingham, UK), somatostatin (sc‐25262, Santa Cruz, Insight Biotechnologies, Wembley, UK), PP (ab77192), NPY1R (ab91262, Abcam, Cambridge, UK), NPY2R (ab31894, Abcam), NPY4R (HPA027863, Sigma), NPY5R (ab32886, Abcam) or DPP‐IV (ab28340, Abcam). The tyramide amplification system was used as a secondary antibody system to improve visualization of PYY in human samples, all NPYR proteins and DPP‐IV (ThermoFisher, Loughborough, UK). All other proteins were visualized using fluorescently‐ and HRP‐labelled secondary antibodies for immunolocalization as follows; anti‐rabbit 488 (A11034, Life Technologies, Loughborough, UK), anti‐mouse TRITC (F5262, Sigma), anti‐guinea pig 648 (ab150187, Abcam), anti‐rabbit HRP (P0448, Dako, Cheadle, UK). Images were visualized using a BioRad (Radiance 2100) confocal microscope.
Ab32886
Manufactured by Abcam
Sourced in United Kingdom
Ab32886 is a laboratory reagent that can be used for various biological applications. It is a lyophilized powder form and requires reconstitution prior to use. The core function of this product is to serve as a research tool, but its specific intended use should not be extrapolated without further information.
Automatically generated - may contain errors
Lab products found in correlation
Ab91262, by Abcam (1 mentions)
Ab28340, by Abcam (1 mentions)
Ab150187, by Abcam (1 mentions)
Ab77192, by Abcam (1 mentions)
P0448, by Agilent Technologies (1 mentions)
A11034, by Thermo Fisher Scientific (1 mentions)
G2654, by Merck Group (1 mentions)
F5262, by Merck Group (1 mentions)
Radiance 2100, by Bio-Rad (1 mentions)
Ab30914, by Abcam (1 mentions)
Pt link system, by Agilent Technologies (1 mentions)
M7240, by Agilent Technologies (1 mentions)
2 protocols using ab32886
1
Multimarker Immunohistochemistry of Pancreatic Islets
2
Immunohistochemical Analysis of NPY Receptor Expression
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A ready-to-use system was used to perform the immunohistochemical reactions: DAKO EnVision™ FLEX+, Mouse, Low pH (Link), catalogue number K8002, and PT-Link system (Dako). The following antibodies were used: anti-NPY (ab30914, polyclonal, rabbit, 1:2000, Abcam), anti-NPY1R (ab183108, polyclonal, rabbit, 1:25, Abcam), anti-NPY2R (PA5-41576, polyclonal, rabbit, 1:400, Thermo Fisher), anti-NPY5R (ab32886, polyclonal, goat, 1:100, Abcam), anti-ERG (ab136152, monoclonal, mouse, 1:20, Abcam), anti-Ki67 (M7240, monoclonal, mouse, DAKO, Ready-to-Use). Antibody Diluent with Background Reducing Components (DAKO, Code S3022) was used. The procedures were performed according to a standard immunostaining procedure, with primary antibody incubation for 30 min at room temperature. Positive and negative controls recommended by the manufacturers were used.
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