The largest database of trusted experimental protocols

Anti mouse cd62p fitc

Manufactured by BD
Sourced in United Kingdom

Anti-mouse CD62P-FITC is a fluorescent-labeled antibody specific for the mouse CD62P (P-selectin) antigen. CD62P is a cell adhesion molecule expressed on the surface of activated platelets and endothelial cells. This antibody can be used to detect and quantify mouse CD62P-positive cells in flow cytometry applications.

Automatically generated - may contain errors

2 protocols using anti mouse cd62p fitc

1

Platelet Activation by ADP and EcoHIV

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse whole blood was treated with either 10 μM ADP or 1.0x105 pg p24 EcoHIV for 30 min, 1 hr, and 2 hrs at 37°C. Subsequently, blood was processed and analyzed for platelet activation by flow cytometry. Briefly, whole blood was fixed with 4% paraformaldehyde and washed twice with 1 ml staining buffer (1x PBS containing 2% BSA). Red blood cells (RBCs) were then lysed using ACK lysis buffer (Life Technologies, Grand Island, NY) and the remaining cells were washed twice in staining buffer. Cells were stained with 2.5 μl anti-mouse CD61-PE (AbD Serotec, Oxford, U.K.) and 1 μl anti-mouse CD62P-FITC (BD Biosciences, San Jose, CA). Following the staining, samples were acquired using a flow cytometer (Accuri C6; Accuri Cytometers, Ann Arbor, MI). Platelets were gated based on forward and side scatter, followed by assessment of the platelet activation marker CD62P. Sizing beads (Mega Mix; BioCytex, Marseille, France) were used to delineate the platelet gate (0.9–3 μm). Unstained cells and cells stained with only CD61 were used as controls. 20,000 events were acquired to measure platelet percentages and activation in terms of CD62P expression.
+ Open protocol
+ Expand
2

Platelet Activation Assay in Human and Mouse Blood

Check if the same lab product or an alternative is used in the 5 most similar protocols
Platelet activation was assessed in human whole blood via CD62P (P-selectin) surface expression, using a previously described method [11 (link), 13 (link)], within 1h of the blood draw. Similarly, platelet activation in mouse blood was assessed using 2.5μL anti-mouse CD61-PE (catalog # 561910) and 1μL anti-mouse CD62P-FITC (catalog # 561923; BD Biosciences, San Jose, CA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!