The growth rate of Lb. rhamnosus yoba in the traditional maheu was determined at t = 0, 12, 24, and 36 h. One millilitre of the sample was aseptically taken from the maheu at a 12 hourly interval. Thereafter, serial decimal dilutions were carried out in peptone physiological salt solution (pH 7, 8.5 g / L NaCl, and 1 g / L neutralized bacteriological peptone from Oxoid). Diluents of 100 µL were plated onto de Man, Rogosa, and MRS agar (1.2 % agar, bacteriological peptone from Oxoid, added to de Man, Rogosa, and Sharpe broth, Merck) in triplicate. MRS agar plates were incubated at 37 °C under anaerobic conditions in Gas Pack anaerobic jars (Becton Dickinson Microbiology Systems, Baltimore, Maryland, USA). Colonies on MRS agar were counted and expressed as colony-forming units per milliliter (CFU / mL) of Lb. rhamnosus yoba.
Bacteriological peptone
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, Italy, United States, Germany
Bacteriological peptone is a type of microbiological culture medium component used in the preparation of culture media for the growth and isolation of various bacteria. It serves as a source of organic nitrogen, amino acids, and other nutrients required for the cultivation of diverse bacterial species.
Automatically generated - may contain errors
Lab products found in correlation
Yeast extract, by Thermo Fisher Scientific (10 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (4 mentions)
Sodium chloride (nacl), by Avantor (4 mentions)
Dextrose, by Merck Group (3 mentions)
Sodium chloride (nacl), by Merck Group (3 mentions)
Peptone physiological salt solution, by Thermo Fisher Scientific (2 mentions)
Gas pack anaerobic jars, by BD (2 mentions)
Mrs broth, by Thermo Fisher Scientific (2 mentions)
Malt extract, by Thermo Fisher Scientific (2 mentions)
Nutrient agar, by Thermo Fisher Scientific (2 mentions)
Potato dextrose agar (pda), by Thermo Fisher Scientific (2 mentions)
Soluble starch, by Thermo Fisher Scientific (2 mentions)
1 butanol, by Avantor (2 mentions)
Glycerol, by Merck Group (2 mentions)
Yeast extract, by Merck Group (2 mentions)
Tween 80, by Merck Group (2 mentions)
Na2hpo4, by Merck Group (2 mentions)
Ch3coona, by Merck Group (2 mentions)
Mgso4, by Merck Group (2 mentions)
Mnso4, by Merck Group (2 mentions)
47 protocols using bacteriological peptone
1
Enumeration of Lb. rhamnosus yoba in Maheu
2
Probiotic and Yeast Strain Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The probiotic and yeast strain used in this study were Lactobacillus rhamnosus HN001 (Danisco A/S, Copenhagen, Denmark) and Saccharomyces cerevisiae EC-1118 (Lallemand Pty, Ontario, Canada), respectively.
Lactobacillus rhamnosus HN001 cells were grown by inoculating 1% (v/v) frozen stock culture into de Man, Rogosa, Sharpe (MRS) broth (Oxoid Ltd., Hampshire, England), followed by static incubation at 37 °C for 24 h. Yeast cells were propagated by inoculating 1% (v/v) frozen S. cerevisiae EC-1118 stock culture into yeast-malt (YM) broth (10 g/L dextrose (Sigma-Aldrich, Oakville, Ontario, Canada), 3 g/L yeast extract, 3 g/L malt extract and 5 g/L bacteriological peptone (all from Oxoid Ltd.), that was acidified to pH 5.0 with 1 M HCl and incubated statically at 30 °C for 24 h.
After two consecutive transfers, the microbial cultures were centrifuged (8000×g, 10 min, 4 °C) and washed twice with sterile 0.85% (w/v) NaCl. Working cultures were obtained by re-suspending the washed pellets to their initial volume with phosphate-buffered saline (PBS) that was acidified to pH 3.0 using 90% lactic acid (Merck, Darmstadt, Germany). All pH measurements were made using a Metrohm 713 pH meter (Herisau, Switzerland) calibrated with pH 4 and 7 buffers from Merck.
Lactobacillus rhamnosus HN001 cells were grown by inoculating 1% (v/v) frozen stock culture into de Man, Rogosa, Sharpe (MRS) broth (Oxoid Ltd., Hampshire, England), followed by static incubation at 37 °C for 24 h. Yeast cells were propagated by inoculating 1% (v/v) frozen S. cerevisiae EC-1118 stock culture into yeast-malt (YM) broth (10 g/L dextrose (Sigma-Aldrich, Oakville, Ontario, Canada), 3 g/L yeast extract, 3 g/L malt extract and 5 g/L bacteriological peptone (all from Oxoid Ltd.), that was acidified to pH 5.0 with 1 M HCl and incubated statically at 30 °C for 24 h.
After two consecutive transfers, the microbial cultures were centrifuged (8000×g, 10 min, 4 °C) and washed twice with sterile 0.85% (w/v) NaCl. Working cultures were obtained by re-suspending the washed pellets to their initial volume with phosphate-buffered saline (PBS) that was acidified to pH 3.0 using 90% lactic acid (Merck, Darmstadt, Germany). All pH measurements were made using a Metrohm 713 pH meter (Herisau, Switzerland) calibrated with pH 4 and 7 buffers from Merck.
3
Microbial Culture Media Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glucose, sodium hydroxide (NaOH), and phosphate buffer saline (PBS) were purchased from Sigma Aldrich (St Louis, MO, United States). Nutrient agar and bacteriological peptone, citric acid, and yeast extract were supplied by the Oxoid Ltd., while dextrose by Daejung, South Korea. Curcumin and sodium dihydrogen phosphate (NaH2PO4) purchased from Merck & Company, Inc. (Darmstadt, Germany).
4
Lipase Production from Residual Frying Oil
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bacteriological peptone and yeast extract were purchase from Oxoid (Hampshire, UK). Glucose and agar-agar were supplied by Vetec (Rio de Janeiro, Brazil). Antifoan 204 was obtained from Sigma-Aldrich (St. Louis, MO, USA) to reduce foaming. The Residual Frying Oil (RFO), used as an inductor for lipase production, was supplied by the Brazil Fast Food Corporation, used in Bob’s fast food restaurant (Rio de Janeiro, Brazil) to fry potatoes. Pasteurized nonfermented unsalted butter produced from bovine milk (Bela nata, Laticínios Passa Quatro, Minas Gerais, Brazil) was obtained in local market (Rio de Janeiro, Brazil). Saturated fatty acid ethyl esters (FAEEs) of caprylic (8:0), capric (10:0), lauric (12:0), myristic (14:0), palmitic (16:0), and stearic (18:0) acids, unsaturated FAEEs of oleic (18:1n-9), linoleic (18:2n-6), and α-linolenic (18:3n-3) FAs, triolein standard and fatty acid methyl esters (FAMEs) standards for gas chromatography (GC), all of >99% purity, were purchased from Sigma-Aldrich (Saint Quentin, France).
5
Motility Assay for Bacterial Strains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The motility of the strains was tested as described previously (Van Boeijen et al., 2010 (link)). Briefly, semisolid medium containing 0.25% (wt/vol) agar (Oxoid), 1% (wt/vol) bacteriological peptone (Oxoid), 0.5% (wt/vol) NaCl (Merck), 0.005% (wt/vol) 2,3,5-triphenyltetrazolium chloride (Sigma–Aldrich, The Netherlands) was inoculated by stabbing a single colony into the medium. After 3 days of incubation at 30∘C, motile strains showed a red cloudy pattern as a result of the reduction of 2,3,5- triphenyltetrazolium chloride to formazan caused by bacterial metabolism. The LO28 WT and immotile HHP variant 17 (Van Boeijen et al., 2010 (link)) were used as positive and negative control, respectively. Isolates which showed no or reduced motility at 30∘C were retested at 25∘C to confirm reduced motility, since it is know that motility is temperature dependent (Gründling et al., 2004 (link)).
6
Yeast and Lactic Acid Bacteria Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The yeasts strains D. bruxellensis CBS 11270 and S. cerevisiae J672 9 (link) and the LAB strain L. vini 2FLAB5 isolated from a Swedish ethanol plant 6 (link) were used in this study.
L. vini was pregrown on Man–Rogosa–Sharpe (MRS) agar (Oxoid, Basingstoke, New Hampshire, England) in an anaerobic environment using a BD BBL™ GasPak™ Plus Anaerobic System (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).
Yeast extract–peptone–dextrose medium (YPD) contained 10 g/L yeast extract, 20 g/L bacteriological peptone (both from Oxoid), and 20 g/Ld (+) glucose (VWR International Ltd., Poole, England). Solid media also contained 16 g/L agar. For testing the influence of ethanol on flocculation, 10, 50, or 100 g/L ethanol were added to liquid YPD. Synthetic medium contained KH2PO4 18.75 g/L, (NH4)2HPO4 6 g/L, MgSO4·7H2O 1.13 g/L, yeast nitrogen base (Difco™; Becton Dickinson and Company) 6.5 g/L, and glucose 40 g/L. Several supplements of synthetic medium were tested: 5 g/L yeast extract or a mixture of ergosterol (18 µg/L) and Tween 80 (3 g/L) combined with 20 g/L bacteriological peptone or all 20 proteinogenic amino acids (100 mg/L each). The ergosterol/Tween 80 solution was prepared according to Ref. [13 ].
L. vini was pregrown on Man–Rogosa–Sharpe (MRS) agar (Oxoid, Basingstoke, New Hampshire, England) in an anaerobic environment using a BD BBL™ GasPak™ Plus Anaerobic System (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).
Yeast extract–peptone–dextrose medium (YPD) contained 10 g/L yeast extract, 20 g/L bacteriological peptone (both from Oxoid), and 20 g/L
7
Simulated Gastric Fluid Assay for Bacterial Survival
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stationary-phase cultures grown overnight at 37°C in 4 ml LB were hundredfold diluted in test tubes containing 4 ml of simulated gastric fluid (Beumer et al., 1992 (link)). The composition of this medium was 8.3 g/l bacteriological peptone, 3.5 g/l glucose, 2.05 g/l NaCl, 0.6 g/l KH2PO4, 0.11 g/l CaCl2, 0.37 g/l KCl, 0.05 g/l bile salts (Oxoid, Basingstoke, UK), 0.1 g/l lysozyme (66200 U/mg, Fluka, Buchs, Switzerland), and 13.3 mg/l pepsin (47 U/g, Fluka, Buchs, Switzerland), and the pH was adjusted to 2.50 with HCl. The suspensions were incubated at 37°C for 6 h. Every hour, 100 μl samples were taken, serially diluted in potassium phosphate buffer (10 mM; pH 7.00) and subsequently plated on LB agar. D-values (time required for a 10-fold reduction in viable cells) were determined by identification of the log-linear Bigelow model (for the ΔwaaC mutant) or the log-linear model with shoulder (for the wild-type and other deletion mutants) using GInaFiT (Geeraerd et al., 2005 (link)).
8
Halobacterium salinarum NRC-1 Growth Curve Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Halobacterium salinarum NRC-1 were grown in complex media (CM) (250 g/L NaCl, 20 g/L MgSO4, 2 g/L KCl, 3 g/L Sodium citrate, 10 g/L bacteriological peptone (Oxoid)) at 37°C, under light and constant agitation of 125 rpm. Samples were taken at 3 different time points in a growth curve: middle of exponential phase (17h, OD600 ~ 0.3, aka early-log phase [4 ]), end of stationary phase (37h, OD600 ~ 0.5, aka mid-log phase) and gas vesicles release phase (86h, OD600 ~ 1.1, aka late-log phase) (Fig. S1). Reference samples were cultured under standard growth conditions (37ºC, 225 rpm, constant light) and sampled at mid-log phase (OD600 ~ 0.5) [80 ].
9
Longan Drying and Amino Acid Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The major material utilized in this study was dried whole longan, purchased from a local market at Lamphun, Thailand, which was dried by a hot air dryer at 80°C for 17 hr, then at 75°C for 20 hr, then at 65°C for 10 hr, and finally stored at 4°C until analysis.
Potato dextrose agar and bacteriological peptone were purchased from Oxoid.dl ‐Malic acid was purchased from Sigma‐Aldrich. Alanine, arginine, aspartic acid, gamma‐aminobutyric acid, glutamic acid, glycine, isoleucine, leucine, lysine, phenylAlanine, proline, serine, threonine, tyrosine, and valine were supplied by Sigma Chemicals. AccQ‐Fluor reagent kits containing borate buffer, reagent powder, and acetonitrile were supplied by Waters Co. Ltd.
Potato dextrose agar and bacteriological peptone were purchased from Oxoid.
10
Isolation and Cultivation of Marine Bacteria
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Streptomyces mobaraensis DSM 40847 was purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany). The marine bacteria V. alginolyticus B1, Erythrobacter sp. HKB8, Ruegeria B32, S. aureus B04, S. hominis N32, S. arlettae OM, M. esteraromaticum N22, I. sediminum N28, Pseudoalteromonas L001, and E. coli N57 were isolated from marine biofilms and obtained from the culture collection of our laboratory [24 (link)]. Soybean powder was purchased from Wugumf, Shenzhen, China. Soluble starch was purchased from Affymetrix, Santa Clara, CA, USA. Magnesium sulfate hydrate was purchased from Riedel-de-Haën, Seelze, Germany. Bacteriological peptone was obtained from Oxoid, Milan, Italy. Mueller-Hinton broth (MHB) was purchased from Fluka Chemie AG, Buchs, Switzerland. Phosphate-buffered saline (PBS) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Thermo Fisher Scientific Inc., San Jose, CA, USA. Lysogeny broth (LB), glucose, and 1-butanol were purchased from VWR International Ltd., Leicestershire, UK. All other chemicals were supplied by Sigma-Aldrich Corporation, Saint Louis, MO, USA.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!