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Bio plex magnetic bead assay

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The Bio-Plex magnetic bead assay is a multiplex detection system that allows for the simultaneous measurement of multiple analytes in a single sample. The core function of this lab equipment is to facilitate the quantitative analysis of a variety of biomolecules, including proteins, cytokines, and other targets, using magnetic beads coated with specific capture agents.

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Lab products found in correlation

Luminex, by R&D Systems (2 mentions) Elisa, by R&D Systems (2 mentions) Bio plex 200, by Bio-Rad (2 mentions)

2 protocols using bio plex magnetic bead assay

1

Cytokine Profiling in Lung Tissues

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Cytokine concentrations in BALF were assessed using a Bio-plex magnetic bead assay (Luminex, R&D) and analyzed using a Bio-Plex 200 workstation (Bio-Rad). The cytokine panel included granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), CXCL1, CXCL2, CXCL5, CCL20, IL-23p19, IL-17A, and TNF-α. Whole lung IL-17A and IFN-γ protein concentrations were measured by enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis, MN) following protein extraction from lung homogenates58 (link). Cytokine concentrations in ex vivo CD4+ T cell stimulation supernatants were measured by Luminex (for IL-17A, IFN-γ, IL-2, TNF-α, IL-4, and IL-10; R&D Systems) or ELISA (for IL-22; R&D Systems).
Smith N.M., Wasserman G.A., Coleman F.T., Hilliard K.L., Yamamoto K., Lipsitz E., Malley R., Dooms H., Jones M.R., Quinton L.J, & Mizgerd J.P. (2017). Regionally Compartmentalized Resident Memory T Cells Mediate Naturally Acquired Protection Against Pneumococcal Pneumonia. Mucosal immunology, 11(1), 220-235.
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2

Cytokine Profiling in Lung Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokine concentrations in BALF were assessed using a Bio-plex magnetic bead assay (Luminex, R&D) and analyzed using a Bio-Plex 200 workstation (Bio-Rad). The cytokine panel included granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), CXCL1, CXCL2, CXCL5, CCL20, IL-23p19, IL-17A, and TNF-α. Whole lung IL-17A and IFN-γ protein concentrations were measured by enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis, MN) following protein extraction from lung homogenates58 (link). Cytokine concentrations in ex vivo CD4+ T cell stimulation supernatants were measured by Luminex (for IL-17A, IFN-γ, IL-2, TNF-α, IL-4, and IL-10; R&D Systems) or ELISA (for IL-22; R&D Systems).
Smith N.M., Wasserman G.A., Coleman F.T., Hilliard K.L., Yamamoto K., Lipsitz E., Malley R., Dooms H., Jones M.R., Quinton L.J, & Mizgerd J.P. (2017). Regionally Compartmentalized Resident Memory T Cells Mediate Naturally Acquired Protection Against Pneumococcal Pneumonia. Mucosal immunology, 11(1), 220-235.
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