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Ab6102

Manufactured by Abcam
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Ab6102 is a primary antibody that recognizes the target protein. It is designed for use in various laboratory applications.

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Lab products found in correlation

Alexafluor plus 555 goat anti mouse igg secondary antibody, by Thermo Fisher Scientific (2 mentions) Ez magna rip rna binding protein immunoprecipitation kit, by Merck Group (2 mentions) Gcs200621, by Merck Group (2 mentions) Ab107866, by Merck Group (2 mentions) Ab38150, by Abcam (2 mentions) Ab154989, by Abcam (2 mentions) Ab94950, by Merck Group (2 mentions) Ab70522, by Abcam (2 mentions) Mabn45, by Abcam (2 mentions) Ab4791, by Abcam (2 mentions) Ab133497, by Abcam (1 mentions) Ab1318, by Abcam (1 mentions) Ab1791, by Abcam (1 mentions) Western lighting chemiluminescence reagent, by PerkinElmer (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Merck Group (1 mentions) Ab82843, by Abcam (1 mentions) Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti mhc mf 20, by Developmental Studies Hybridoma Bank (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions)

Close spelling variants of this product

Anti-hnRNPA2B1 antibody (ab6102)

10 protocols using ab6102

1

Immunofluorescence and Western Blot Analyses

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Primary antibodies used for immunofluorescence assays were: mouse mAb anti-glycoprotein E of DENV (ab41349, Abcam), mouse mAb SA02-BG12 anti N protein of JUNV (Sánchez et al., 1989) , mouse mAb anti-hnRNP A2 (ab6102, Abcam), rabbit Ab anti-hnRNP K (R332, Cell Signaling), rabbit Ab anti-T7 (ab9115, Abcam) and rabbit immunosera obtained in our laboratory: anti-DENV-2, anti-JUNV and anti-VSV. Goat anti-mouse IgG conjugated to fluorescein isothiocyanate (FITC) or rhodamine (TRITC), used as secondary antibodies were purchased from Sigma-Aldrich.
Western blot analysis was performed employing mouse mAb anti-hnRNP A2 (ab6102, Abcam), rabbit Ab anti hnRNP K (R332, Cell Signalling), mouse mAb anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH, ab8245, Abcam), rabbit Ab anti-T7 (ab9115, Abcam) or rabbit Ab anti-histone H4 (sc-8660 R, Santa Cruz Biotechnology) as primary antibodies and peroxidase anti-rabbit IgG (W4011, Promega) or peroxidase anti-mouse Ig G (A9044, Sigma-Aldrich) as secondary antibodies.
Brunetti J.E., Scolaro L.A, & Castilla V. (2015). The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication. Virus research, 203.
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2

Western Blot Analysis of EV Markers

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50 μL of EV samples from Sham and TAC EV was directly mixed with 5x SDS sample buffer, boiled at 95°C for 3 minutes and then separated on a SDS‐PAGE gel. The total loaded protein amount was calculated by the intensity of Coomassie blue stain. The membrane was blocked in 5% skim milk solution and incubated overnight with an antibody directed against hnRNA A2B1 (Ab6102, Abcam, 1:5000), CD63 (Ab1318, Abcam, 1:1000), Flotillin 1 (Ab133497, Abcam, 1:10 000), TSG101 (Ab83, Abcam, 1:1000), H3 (Ab1791, Abcam, 1:1000) and SERCA2a (custom antibody from 21st Century Biochemicals, 1:3000). The membrane was then incubated with a horseradish peroxidase‐conjugated secondary antibody (Sigma) and developed with Western Lighting chemiluminescence reagent (PerkinElmer).
Oh J.G., Lee P., Gordon R.E., Sahoo S., Kho C, & Jeong D. (2020). Analysis of extracellular vesicle miRNA profiles in heart failure. Journal of Cellular and Molecular Medicine, 24(13), 7214-7227.
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3

Immunofluorescence Assay for hnRNPA2B1

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Cancer cells were seeded onto sterile slides into 24-well culture plates. Then, fixed cells with 4% paraformaldehyde for 20 min when reached a confluence of 80%. Following permeabilized membranes with 0.1% Triton X-100 for 10 min, blocked antigens with 10% goat serum for 30 min in room temperature. Cells were then incubated with anti-hnRNPA2B1 (ab6102, Abcam) at a 1:100 dilution overnight at 4 °C, followed by further incubation at room temperature for 1 h with AlexaFluor Plus 555 goat anti-mouse IgG secondary antibody (A32727, Thermo) and then labeled DNA with DAPI for 10 min.
Zhang Y., Huang W., Yuan Y., Li J., Wu J., Yu J., He Y., Wei Z, & Zhang C. (2020). Long non-coding RNA H19 promotes colorectal cancer metastasis via binding to hnRNPA2B1. Journal of Experimental & Clinical Cancer Research : CR, 39, 141.
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4

Immunocytochemistry of Myogenic Markers

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C2C12 myoblast cells were washed with PBS in a laminar flow hood and fixed with 4% paraformaldehyde for 10 min at RT in a chemical hood. Cells were permeabilized with 0.25% Triton-X100 in PBS containing 2% bovine serum albumin (Sigma) for 1 hr at RT. Cells were incubated with primary antibody at 4°C overnight, then incubated with secondary antibody at RT for 1 hr. Primary antibodies included mouse anti-Hnrnpa2b1 (ab6102, Abcam) at 1:200, mouse-anti-myogenin (ab82843, Abcam), and a mouse anti-MHC MF-20 (Developmental Studies Hybridoma Bank, University of Iowa) at undiluted, ‘neat’ concentration. Alexa Fluor secondary antibodies (Molecular Probes) were used at a 1:1,000 dilution.
Wheeler J.R., Whitney O.N., Vogler T.O., Nguyen E.D., Pawlikowski B., Lester E., Cutler A., Elston T., Dalla Betta N., Parker K.R., Yost K.E., Vogel H., Rando T.A., Chang H.Y., Johnson A.M., Parker R, & Olwin B.B. (2022). RNA-binding proteins direct myogenic cell fate decisions. eLife, 11, e75844.
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5

RIP Assay for hnRNPA2B1 RNA Binding

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EZ-Magna RIP RNA Binding Protein Immunoprecipitation Kit (17–700, Millipore) was used to perform RIP assays following the protocol. Anti-hnRNPA2B1 (ab6102, Abcam) antibody and normal mouse Ig (GCS200621, Millipore) were used to immunoprecipitated with target RNA or as a negative control. Finally, RNA was subjected to qRT-PCR analysis as described above to detect the enrichment between indicated RNA binding to hnRNPA2B1.
Zhang Y., Huang W., Yuan Y., Li J., Wu J., Yu J., He Y., Wei Z, & Zhang C. (2020). Long non-coding RNA H19 promotes colorectal cancer metastasis via binding to hnRNPA2B1. Journal of Experimental & Clinical Cancer Research : CR, 39, 141.
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6

Antibodies for Neurodegeneration Assays

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Anti-hnRNP A1 antibodies were obtained from Abcam (ab4791- rabbit polyclonal, ab5832 - mouse monoclonal) and Millipore (05-1521- mouse monoclonal; 04-1469 – mouse monoclonal). The anti-hnRNP A1 antibodies are specific for hnRNP A1-M9 and have been shown to overlap the M9 immunodominant epitope recognized by IgG isolated from MS patients [24 (link)]. Isotype-matched anti-rabbit IgGs were obtained from Abcam (ab107866) and Millipore (12-370). Antibodies were added to the culture media of SK-N-SH cells at a concentration of 8 ug/ml for each assay as determined in previous studies [12 ]. Primary antibodies utilized for protein detection by Western blot were as follows: spastin (SPG4-Abcam ab38150), paraplegin (SPG7-Abcam ab154989), spartin (SPG20-Abcam ab94950), and hnRNP A1-(Millipore 05-1521). Antibodies were used at manufacturers recommended concentration. Antibodies utilized for immunochemistry in granule studies were as follows: TAR-DNA Binding Protein (TDP-43 (Millipore MABN45)), GW-182 (Abcam ab70522), or hnRNP A2/B1 (Abcam ab6102).
Douglas J.N., Gardner L.A., Salapa H.E, & Levin M.C. (2016). Antibodies to the RNA Binding Protein Heterogeneous Nuclear Ribonucleoprotein A1 Colocalize to Stress Granules Resulting in Altered RNA and Protein Levels in a Model of Neurodegeneration in Multiple Sclerosis. Journal of clinical & cellular immunology, 7(2), 402.
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7

Western Blot Analysis of Hnrnpa2b1

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Western blot was performed according to standard protocols. Equal volumes (20 µl) of fractions were then resolved on a 4–12% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane (Bio-Rad). Primary antibodies included mouse anti-Hnrnpa2b1 (1:200; ab6102, Abcam) and monoclonal rabbit anti-GAPDH (14C10) conjugated to HRP (1:2000; Cell Signaling, 3683S).
Wheeler J.R., Whitney O.N., Vogler T.O., Nguyen E.D., Pawlikowski B., Lester E., Cutler A., Elston T., Dalla Betta N., Parker K.R., Yost K.E., Vogel H., Rando T.A., Chang H.Y., Johnson A.M., Parker R, & Olwin B.B. (2022). RNA-binding proteins direct myogenic cell fate decisions. eLife, 11, e75844.
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8

Antibodies for Neurodegeneration Assays

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Anti-hnRNP A1 antibodies were obtained from Abcam (ab4791- rabbit polyclonal, ab5832 - mouse monoclonal) and Millipore (05-1521- mouse monoclonal; 04-1469 – mouse monoclonal). The anti-hnRNP A1 antibodies are specific for hnRNP A1-M9 and have been shown to overlap the M9 immunodominant epitope recognized by IgG isolated from MS patients [24 (link)]. Isotype-matched anti-rabbit IgGs were obtained from Abcam (ab107866) and Millipore (12-370). Antibodies were added to the culture media of SK-N-SH cells at a concentration of 8 ug/ml for each assay as determined in previous studies [12 ]. Primary antibodies utilized for protein detection by Western blot were as follows: spastin (SPG4-Abcam ab38150), paraplegin (SPG7-Abcam ab154989), spartin (SPG20-Abcam ab94950), and hnRNP A1-(Millipore 05-1521). Antibodies were used at manufacturers recommended concentration. Antibodies utilized for immunochemistry in granule studies were as follows: TAR-DNA Binding Protein (TDP-43 (Millipore MABN45)), GW-182 (Abcam ab70522), or hnRNP A2/B1 (Abcam ab6102).
Douglas J.N., Gardner L.A., Salapa H.E, & Levin M.C. (2016). Antibodies to the RNA Binding Protein Heterogeneous Nuclear Ribonucleoprotein A1 Colocalize to Stress Granules Resulting in Altered RNA and Protein Levels in a Model of Neurodegeneration in Multiple Sclerosis. Journal of clinical & cellular immunology, 7(2), 402.
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9

hnRNPA2B1 RNA Binding Immunoprecipitation

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EZ-Magna RIP RNA Binding Protein Immunoprecipitation Kit (17-700 , Millipore) was used to perform RIP assays following the protocol. Anti-hnRNPA2B1 (ab6102, Abcam) antibody and normal mouse Ig (GCS200621, Millipore) were used to immunoprecipitated with target RNA or as a negative control.
Finally, RNA was subjected to qRT-PCR analysis as described above to detect the enrichment between indicated RNA binding to hnRNPA2B1.
Zhang Y., Huang W., Yuan Y., Li J., Wu J., Yu J., He Y., Wei Z., & Zhang C. (2020). Long non-coding RNA H19 promotes colorectal cancer metastasis via binding to hnRNPAA2B1.
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10

Immunofluorescent Localization of hnRNPA2B1

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Cancer cells were seeded onto sterile slides into 24-well culture plates. Then, xed cells with 4% paraformaldehyde for 20 min when reached a con uence of 80%. Following permeabilized membranes with 0.1% Triton X-100 for 10 min, blocked antigens with 10% goat serum for 30 min in room temperature.
Cells were then incubated with anti-hnRNPA2B1 (ab6102, Abcam) at a 1:100 dilution overnight at 4 °C, followed by further incubation at room temperature for 1 h with AlexaFluor Plus 555 goat anti-mouse IgG secondary antibody (A32727, Thermo) and then labeled DNA with DAPI for 10 min.
Zhang Y., Huang W., Yuan Y., Li J., Wu J., Yu J., He Y., Wei Z., & Zhang C. (2020). Long non-coding RNA H19 promotes colorectal cancer metastasis via binding to hnRNPAA2B1.
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