Mouse embryonic stem cell lines for imaging the Pou5f1 and Nanog loci were derived from Bruce 4 mESCs (Millipore Sigma CMTI-2; murine strain C57/BL6J, male – species/sex verified by karyotyping, no additional cell line authentification performed). OMG mESCs39 (link) contain a 24 × MS2 cassette integrated in the 3′-UTR of one of the two Pou5f1 alleles57 (link) and also stably express MCP-mNeonGreen39 (link). NMG mESCs contain 24 × MS2 cassettes integrated in the 3′-UTR of both Nanog alleles and also stably express MCP-mNeonGreen58 (link). NMG SNAP-Brd4 clone 7 cells also contain bi-allelic integrations of SNAP-tag at the Brd4 locus39 (link). mESC lines for imaging the Sox2 locus were derived from B6 albino mESCs (B6(Cg)-Tyrc-2J/J, Jackson Lab 000058; male - species/sex verified by karyotyping, no additional cell line authentification performed). All cell lines tested negative for mycoplasma.
Sigma cmti 2
Manufactured by Merck Group
The Sigma CMTI-2 is a compact and versatile laboratory instrument designed for chemical and materials analysis. It provides high-resolution imaging and precise analytical capabilities for a wide range of applications. The core function of the Sigma CMTI-2 is to enable detailed characterization of samples at the micro- and nanoscale levels.
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Lab products found in correlation
3 protocols using sigma cmti 2
1
Mouse Embryonic Stem Cell Lines for Imaging
2
Molecular Tagging of Stem Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mouse embryonic stem cell lines were derived from Bruce 4 mESCs (Millipore Sigma CMTI-2; murine strain C57/BL6J, male – species/sex verified by karyotyping, no additional cell line authentification performed). KMG mESCs contain 24 × MS2 cassettes integrated in the 3′-UTR of both Klf4 alleles and stably express MCP-mNeonGreen. SMG mESCs(11 ) contain 24 × MS2 cassettes integrated in the 3′-UTR of one Sox2 alleles and stably express MCP-mNeonGreen.
3
Mouse Embryonic Stem Cell Lines for Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse embryonic stem cell lines for imaging the Pou5f1 and Nanog loci were derived from Bruce 4 mESCs (Millipore Sigma CMTI-2; murine strain C57/BL6J, male – species/sex verified by karyotyping, no additional cell line authentification performed). OMG mESCs39 (link) contain a 24 × MS2 cassette integrated in the 3′-UTR of one of the two Pou5f1 alleles57 (link) and also stably express MCP-mNeonGreen39 (link). NMG mESCs contain 24 × MS2 cassettes integrated in the 3′-UTR of both Nanog alleles and also stably express MCP-mNeonGreen58 (link). NMG SNAP-Brd4 clone 7 cells also contain bi-allelic integrations of SNAP-tag at the Brd4 locus39 (link). mESC lines for imaging the Sox2 locus were derived from B6 albino mESCs (B6(Cg)-Tyrc-2J/J, Jackson Lab 000058; male - species/sex verified by karyotyping, no additional cell line authentification performed). All cell lines tested negative for mycoplasma.
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